这篇博客介绍了通过Next Generation Sequencing (NGS) 数据重新分析全基因组测序数据时，发现并研究Chimera的现象。作者建立了一个基于子区间对齐策略的生物信息学管道，定义了两个统计指标，揭示了Chimera的结构特征，并分析了减少未映射Chimera的方法，提高了数据利用效率。
Follow Jimmy:Gene Analysis
探索全基因组测序数据
1.参考文章
Systematic Characteristic Exploration of the Chimeras Generated in Multiple Displacement Amplification through Next Generation Sequencing Data Reanalysis
注释：
Chimera (genetics)
A genetic chimerism or chimera (also spelled chimaera) is a single organism composed of cells from different zygotes. This can result in male and female organs, two blood types, or subtle variations in form.[1] Animal chimeras are produced by the merger of multiple fertilized eggs. In plant chimeras, however, the distinct types of tissue may originate from the same zygote, and the difference is often due to mutation during ordinary cell division. Normally, genetic chimerism is not visible on casual inspection; however, it has been detected in the course of proving parentage.[2]
Another way that chimerism can occur in animals is by organ transplantation, giving one individual tissues that developed from two genomes. For example, a bone marrow transplant can change someone’s blood type.
[1]Norton, Aaron; Ozzie Zehner (2008). “Which Half Is Mommy?: Tetragametic Chimerism and Trans-Subjectivity”. Women’s Studies Quarterly. Fall/Winter: 106–127.
[2] Friedman, Lauren. “The Stranger-Than-Fiction Story Of A Woman Who Was Her Own Twin”. Retrieved 4 August 2014.
Method
We finished data mining towards a series of Next Generation Sequencing (NGS) reads
We established a bioinformatics pipeline based on subsection alignment strategy to discover all the chimeras
最低0.47元/天 解锁文章
扫一扫
被折叠的 条评论
为什么被折叠?
到【灌水乐园】发言
