FaQCs 使用perl编写,R画图。不仅可以统计质量,Q30,还可以trim序列。安装简单准备好两个需要的perl 模块,下载github上的文件,然后直接cd lib;./INSTALL.sh。
https://github.com/LANL-Bioinformatics/FaQCs
fastqc java写的,速度快,结果清晰,但结果没有Q30比值
http://www.bioinformatics.babraham.ac.uk/projects/fastqc/
BGI的Reseqtools 他们说很好用,还没试
https://github.com/BGI-shenzhen/Reseqtools
统计fastq序列数与碱基数
C编写的,速度特别快,下载后直接编译就能用gcc -lz -o kseq_fastq_base kseq_fastq_base.c
https://github.com/billzt/readfq
python统计fastq序列的Q30,可以直接使用gzip的fastq文件
https://github.com/dayedepps/q30
readfq http://not.farbox.com/post/readfq
介绍faqcs的论文https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-014-0366-2
欢迎交流 quan575@qq.com
作者:quan575
链接:https://www.jianshu.com/p/dff6cabb5f94
來源:简书
简书著作权归作者所有,任何形式的转载都请联系作者获得授权并注明出处。
C 语言实现
https://www.cnblogs.com/xudongliang/p/6397627.html