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Nitrates Standard Curve

The document contains data from an experiment measuring absorbance of nitrate solutions at different concentrations. Absorbance was measured at 220 nm and 275 nm, and a corrected value was calculated. A standard curve was generated from the data, with absorbance on the y-axis and concentration on the x-axis, showing a linear relationship with an r-value of 0.9953.

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Christian Joseph Bacani
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16 views

Nitrates Standard Curve

The document contains data from an experiment measuring absorbance of nitrate solutions at different concentrations. Absorbance was measured at 220 nm and 275 nm, and a corrected value was calculated. A standard curve was generated from the data, with absorbance on the y-axis and concentration on the x-axis, showing a linear relationship with an r-value of 0.9953.

Uploaded by

Christian Joseph Bacani
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as XLSX, PDF, TXT or read online on Scribd
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Concentration (ppm)

0.5
1
1.5
2
2.5
6
7
8
9
10

Absorbance
Corrected Value
220 nm
275 nm abs(220nm)-2*abs(275nm)
0.186
0.004
0.178
0.267
0.007
0.253
0.374
0.013
0.348
0.59
0.024
0.542
0.601
0.033
0.535
1.266
0.069
1.128
1.349
0.071
1.207
1.378
0.073
1.232
1.409
0.076
1.257
1.474
0.081
1.312

Data Used
Concentration (ppm) Absorbance
0.5
0.178
1
0.253
1.5
0.348
8
1.378
9
1.409
10
1.474

Percent Accuracy
Corrected value/abs(220nm)
0.956989247
0.947565543
0.930481283
0.918644068
0.890183028
0.890995261
0.894736842
0.894049347
0.892122072
0.89009498

Nitrates Standard Curve


1.8
A
b
s
o
r
b
a
n
c
e

1.6

y = 0.1432385787x + 0.1238071
r=0.9953114258

1.4
1.2
1
0.8
0.6
0.4
0.2
0
0

Concentration

Note:If percent accuracy is less than 90 percent, use the absorbance at 220 nm.
Concentration (ppm)
Absorbance
0.5
0.178
1
0.253
1.5
0.348
2
0.542
2.5
0.601
6
1.266
7
1.349
8
1.378
9
1.409
10
1.474

ard Curve

1432385787x + 0.1238071066
r=0.9953114258

6
Concentration
(ppm)

10

12

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