Diffusion Through A Semipermeable Membrane Lab
Diffusion Through A Semipermeable Membrane Lab
Carley, Vidhu, Kyra Block 2 November 21 2012 Purpose: To demonstrate which molecules diffuse through a semipermeable membrane. Observations: 1. AgNO3 Solution - The Cl ions from the NaCl indicator joined with Ag to form AgCl. The solution turned a pearly white precipitate. 2. Iodine Solution The iodine turned the starch solution a dark blue. 3. Benedicts Solution No change observed, therefore either glucose is not present and/or our solution was contaminated. 4. NaOH/CuSO4 Solution CuSO4 turned NaOH/CuSO4 solution purple. Data:
Biological Molecule Glucose Starch Gelatin (Protein) Chlorine Ion Present in Solution Yes/No No No No Yes Indicator Used Benedicts Solution KI I2 NaOH/CuSo4 AgNo3 Explanation for presence or absence in solution
Questions: 1. The heat of the water acts as a catalyst, which speeds the reaction rate by opening up the membrane therefore allowing the contents to mix. 2. Yes we would expect a positive test for tyrosine in the water bath. The proteins need the help of integral proteins to move through the tubing and digestion will make the protein smaller so it can pass through the pores.
3. The turgidity of the dialysis sack increases over the process. Osmosis was occurring as water was entering the sack causing more substance to be kept within the membrane. 4. I) The heat of the water might break down the starch over time making it the right size to fit through the permeable membrane. II) The membrane itself in the water might expand thus allowing for larger particles to pass through. 5. a) The chlorine ion moved through the semipermeable membrane b) c) The quick movement is important to the human body as it spreads the needed nutrients throughout the body as fast as possible. Conclusion: