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DNA Extraction Procedure

This document outlines a procedure to extract DNA from living organisms. It involves using a salt-detergent solution to lyse cells and release DNA, adding pineapple juice to help precipitate the DNA, then using ethanol to precipitate the DNA out of solution. The extracted DNA can be seen as white strings or clumps after gently twirling a stirring rod in the solution.

Uploaded by

Shairah Mhaey Ramos
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© Attribution Non-Commercial (BY-NC)
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Download as PDF, TXT or read online on Scribd
49 views

DNA Extraction Procedure

This document outlines a procedure to extract DNA from living organisms. It involves using a salt-detergent solution to lyse cells and release DNA, adding pineapple juice to help precipitate the DNA, then using ethanol to precipitate the DNA out of solution. The extracted DNA can be seen as white strings or clumps after gently twirling a stirring rod in the solution.

Uploaded by

Shairah Mhaey Ramos
Copyright
© Attribution Non-Commercial (BY-NC)
Available Formats
Download as PDF, TXT or read online on Scribd
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DNA Extraction Procedure

DNA or deoxyribonucleic acid is a nucleic acid molecule having a double-stranded, helical structure. This particular molecule is what parents pass on to their offspring; hence it is actually the hereditary material. DNA can be found in any living organism from a unicellular organism to a multicellular one like man. This activity will verify that all living organism have DNA, and that DNA can be extracted from them.

Materials:
Source of DNA (e.g. onion, peas, banana, mango, chicken liver, broccoli) Pineapple juice (fresh) Liquid detergent 95% Ethanol (cold) Salt Water Hot plate Basin Thermometer Ice (2) 250 mL beaker (2) 10 mL graduated cylinder Strainer Blender/Mortar and pestle Stirring rod Knife

Procedure:
1. Prepare a salt-detergent solution by combining the 10 mL detergent, 1.5g salt and water bringing to a final volume of 100 mL. 2. Place the solution in water bath of 50-60oC. 3. Cut the sample into small pieces then add to the salt-detergent solution for. The amount of the sample should be half of the volume solution. Stir gently then let it sit for 5 minutes. 4. Add about 3 mL of pineapple juice in the mixture and keep in the water bath for 10 minutes, immediately placing the beaker of solution in ice bath after the prescribed time. Swirl the solution gently. 5. Macerate the mixture using mortar and pestle. 6. Filter the mixture using the strainer above a 250-mL beaker. 7. Tilt the beaker at a 4o angle then slowly and gently add the ice cold ethanol down the inner wall of the beaker. Allow the mixture to sit for 2-3 minutes. The DNA will move to the top. 8. Place the stick into the first layer of the liquid then gently twirl the stick in one direction only. Lift the stick and the string that will be observed is the DNA.

Name: ________________________________________________ Date: __________

Questions:
1. What do you think is the purpose of using blender or mortar and pestle?

2. In what part of the cell did the DNA come from?

3. Describe the appearance of the DNA you collected after the procedure.

4. Would you say that the DNA that you collected is genomic DNA? Explain.

5. Give the function of the following materials used: a. Salt

b. Detergent

c. Pineapple juice

d. Alcohol

Prepared by: Eisha Vienna M. Fernandez

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