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Comparison of Cuticular and Stomatal Transpiration by Four Leaves Method

This document summarizes an experiment comparing cuticular and stomatal transpiration using four leaves. Leaf A served as the control, Leaf B had its upper side covered with petroleum jelly, Leaf C had its lower side covered, and Leaf D had both sides covered. The results showed that Leaves C and D, which had their lower sides covered, were the driest, indicating that the majority of transpiration occurs through the stomata on the lower leaf surface. Blocking the stomata hampered gas exchange and caused water loss.

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Lorenzo Daniel Antonio
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71 views

Comparison of Cuticular and Stomatal Transpiration by Four Leaves Method

This document summarizes an experiment comparing cuticular and stomatal transpiration using four leaves. Leaf A served as the control, Leaf B had its upper side covered with petroleum jelly, Leaf C had its lower side covered, and Leaf D had both sides covered. The results showed that Leaves C and D, which had their lower sides covered, were the driest, indicating that the majority of transpiration occurs through the stomata on the lower leaf surface. Blocking the stomata hampered gas exchange and caused water loss.

Uploaded by

Lorenzo Daniel Antonio
Copyright
© Attribution Non-Commercial (BY-NC)
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Comparison of Cuticular and Stomatal Transpiration by Four Leaves Method

Group 1 - Lorenzo Daniel L. Antonio, Paolo Carlo Calalang, Timothy Carandang, Oneil Joshua Calderon, MHAB1

Submitted to: Maam Elena M. Ragragio Submitted: September 20, 2012 Introduction Transpiration is the evaporation of water through the aerial portions of the plant. Water may evaporate through the cuticle and/or the stomatal apparatus. Four identical leaves are set up: Leaves A, B, C and D Leaf A is the control. Leaf B is covered with petroleum jelly on the upper side and leaf C on the lower side. Leaf D is covered on both sides. Hypothesis Leaf D will be the driest. Leaf C will be drier than Leaf B and Leaf A will be healthy. Objectives: Find out the stomatas function and importance in leaves. Results The leaf with petroleum jelly covered on both sides (Leaf D) and the leaf covered in the lower side (Leaf C) are the driest of the four. The leaf covered in the upper side (Leaf B) was dry but not as dry as C and D. Control leaf (Leaf A) is healthy. Discussion The stomatal pores provide transpiration in leaves so blocking it will hamper the leafs gas exchange and water loss. However, the upper part contains the cuticle which lessens water loss and is reinforced when covered with petroleum jelly but this also covers the stomata which releases water vapor. Stomata are more abundant in the lower side making it important since a majority of gas exchange occurs here. Covering it with the jelly would suffocate the leaf. References 1 Biology 21 General Botany Laboratory Manual, Committee on Biology 21 Laboratory Manual

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