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Descriptive Chart - Proteus Vulgaris

Proteus vulgaris is a rod-shaped, facultative anaerobic bacterium that grows in clumped colonies on nutrient agar and causes hemolysis on blood agar. It is gram-negative, motile, and tests positive for glucose, lactose, and sucrose fermentation as well as gelatin liquefaction, indole production, and nitrate reduction.

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12K views

Descriptive Chart - Proteus Vulgaris

Proteus vulgaris is a rod-shaped, facultative anaerobic bacterium that grows in clumped colonies on nutrient agar and causes hemolysis on blood agar. It is gram-negative, motile, and tests positive for glucose, lactose, and sucrose fermentation as well as gelatin liquefaction, indole production, and nitrate reduction.

Uploaded by

crazybobblaskey
Copyright
© Attribution Non-Commercial (BY-NC)
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
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Descriptive Chart

Culture No.: 61 Organism: Proteus vulgaris

Morphological Characteristics Cell Shape: Bacillus (rod) Arrangement: swarm Size: 0.4 to 0.6 micrometers by 1.2 to 2.5 micrometers Spores: no Grams Stain: negative Motility: yes Capsules: no Cultural Characteristics Nutrient Agar: Colonies clumped together Blood Agar: Positive for hemolysis Agar Slant: positive for growth Nutrient Broth: positive for growth Gelatin Slab: positive for motility and liquefaction Oxygen Requirements: facultative anaerobic Optimum Temp.: 23 C Physiological Characteristics

Tests

Results

IMViC Hydrolysis Fermentation

Glucose Lactose Sucrose Mannitol Gelatin Liquefaction Starch Casein Fat Indole Methyl Red V-P (acetylmethylcarbinol) Citrate Utilization Nitrate Reduction H2S Production Urease Catlase Oxidase DNase Phenylalanase

Yes; yellow No; orange/pink Yes; yellow/orange No; orange/pink Positive Negative Positive Positive Positive (red floating layer) Yes Negative Negative Positive after Zn added Positive (black) Positive (light pink) Yes Negative ?postive (slight green)

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