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Gen PRNP (Pada Huntington's) Primer3 Output: Start Len TM GC% Any 3' Seq

This document contains the output from Primer3 for primers designed for the PRNP gene associated with Huntington's disease. It provides information on 4 potential primer pairs, including the left and right primer sequences, melting temperatures, product sizes, and complementarity statistics. The top ranked primer pair has a left primer sequence of CCAGTCACATTCCTCCCAGT and right primer sequence of ACAAAAACGGAGAGCTGTGG with a product size of 297 base pairs.

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Indah Aprilia
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11 views

Gen PRNP (Pada Huntington's) Primer3 Output: Start Len TM GC% Any 3' Seq

This document contains the output from Primer3 for primers designed for the PRNP gene associated with Huntington's disease. It provides information on 4 potential primer pairs, including the left and right primer sequences, melting temperatures, product sizes, and complementarity statistics. The top ranked primer pair has a left primer sequence of CCAGTCACATTCCTCCCAGT and right primer sequence of ACAAAAACGGAGAGCTGTGG with a product size of 297 base pairs.

Uploaded by

Indah Aprilia
Copyright
© Attribution Non-Commercial (BY-NC)
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
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Gen PRNP (pada Huntingtons) Primer3 Output

No mispriming library specified Using 1-based sequence positions OLIGO start len tm LEFT PRIMER 1220 20 59.96 CCAGTCACATTCCTCCCAGT RIGHT PRIMER 1466 20 59.94 GGTGGAATTGCGAGGTTAAA SEQUENCE SIZE: 1680 INCLUDED REGION SIZE: 1680

gc% 55.00 45.00

any 3.00 4.00

3' seq 1.00 3.00

PRODUCT SIZE: 247, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 1 ATTAAAGATGATTTTTACAGTCAATGAGCCACGTCAGGGAGCGATGGCACCCGCAGGCGG 61 TATCAACTGATGCAAGTGTTCAAGCGAATCTCAACTCGTTTTTTCCGGTGACTCATTCCC 121 GGCCCTGCTTGGCAGCGCTGCACCCTTTAACTTAAACCTCGGCCGGCCGCCCGCCGGGGG 181 CACAGAGTGTGCGCCGGGCCGCGCGGCAATTGGTCCCCGCGCCGACCTCCGCCCGCGAGC 241 GCCGCCGCTTCCCTTCCCCGCCCCGCGTCCCTCCCCCTCGGCCCCGCGCGTCGCCTGTCC 301 TCCGAGCCAGTCGCTGACAGCCGCGGCGCCGCGAGCTTCTCCTCTCCTCACGACCGAGGC 361 AGGTAAACGCCCGGGGTGGGAGGAACGCGGGCGGGGGCAGGGGAGCCGCGGGGGCCGAGT 421 GAGGACCCCGGGCCTCGGGTCCCAGGCGCAAGGGTGCCCGGCCGGGCGGGGTCGGGACCC 481 CAGTGAGGAGGGGCCGGGGGCTGCCCCGCGGGCGCGTGACGCGTCTCGGGCCTGCCCGGC 541 TGCGCTGGTCTCCGCTCGGGTGAGGCGGCTTGGCTTCGCTTTTCAGGTTAGGAAAGCTCC 601 CTTTACTGCGCGTTGGGGGGCTGGGGGAGCTGGCGGAGCCCCGTTAGGGAGGTCGGTGGC 661 GCCGGGGTGTCTCAGCGCCCCCTGCACCCCGCGCGGGTCCGGCCCAGCGGGCGATCGCTG 721 GCGCCCAGGGAACTCCGGGAGGGCCGCCAGCGGGCTCCGCAGGGCGCGGGGCGGGGAGGG

781 GCGCCTGGGGGCCGCGGGGCTCGCGCTCCCCGCCCGTTGGCCGCCCCTCGGAGGCCGAGA 841 TCGGGGCCCAGAACGCCCCTTGGCAAGGCCTGGCGCTTCCGCGATGCCCAGAGGGTGCTT 901 GGGGGGATGGAGAGAGGGGCGCCCGCCGGGGGAGTTCCGGGAGCCTCGGTGCCTCCCGCC 961 GCAGCTGCAGCGTTCCTCCCGGGAGGCGGCCCAGCCCTTCATCCTCGCCGCCTGAGCTTC 1021 TCCGAGGGGGGCTGCAGCCTTGCGGCCGTTGCCACCGCCTGGAGAAGCGGCCCACGCGGA 1081 CTGACGGGCGGGGGCGGGGCCTCGGGCCTCGGCGGGGGCGGGGTCCGGGGAGGCCCCACC 1141 CTCTGTTCTCCAGGGGCGGGGAGAGAGGAGCTGCAGGTCTGCGGCCTGGCCCCAGGTGCG 1201 ATGGCGGACCCCAGCTTGGCCAGTCACATTCCTCCCAGTCCCCCTGGAGGGAGAACGCTG >>>>>>>>>>>>>>>>>>>> 1261 GCCATGGGGGGCTCCAAGGAACAACCAGCCTCGGATGACGACCCTTGGGTCACCGGTCTC 1321 CCCACCTGTGCGGCAGGCGCCTTCACGTTTCATTATTAAACAATGGGGAGAAATCCATGT 1381 TTACTGTCCTTTTTAAGGAATTTTTTGCTCTTCTCTTTGAGGTGGCTGTAGGAAATAGAT 1441 TTTTTTTTTAACCTCGCAATTCCACCACGGTCACATCCATCCTCGCCATCGCAGAGCCAC <<<<<<<<<<<<<<<<<<<< 1501 AGCTCTCCGTTTTTGTTTCCTAGCCTCCAGATTCTCACACAACACAGTGCAGTTTCACTG 1561 CTGTAATGATGAGGATCTTCATGGCCGCGTTATTTTCTTGTTCTGAGAGCATCACGGTTT 1621 AATTAGCAGTTCCCCATATGATTTGAAGTGTTTCCCGTTTCCTTAGGGAAAACTCCTGGT KEYS (in order of precedence): >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start 1 LEFT PRIMER 1337 GCGCCTTCACGTTTCATTAT len 20 tm 60.10 gc% 45.00 any 4.00 3' seq 3.00

RIGHT PRIMER 1466 20 59.94 45.00 4.00 3.00 GGTGGAATTGCGAGGTTAAA PRODUCT SIZE: 130, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00 2 LEFT PRIMER 1220 20 59.96 55.00 3.00 1.00 CCAGTCACATTCCTCCCAGT RIGHT PRIMER 1516 20 60.29 50.00 4.00 0.00 ACAAAAACGGAGAGCTGTGG PRODUCT SIZE: 297, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 3 LEFT PRIMER 1497 20 60.29 50.00 4.00 0.00 CCACAGCTCTCCGTTTTTGT RIGHT PRIMER 1662 20 59.95 45.00 3.00 3.00 GGAAACGGGAAACACTTCAA PRODUCT SIZE: 166, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00 4 LEFT PRIMER 1337 20 60.10 45.00 4.00 3.00 GCGCCTTCACGTTTCATTAT RIGHT PRIMER 1516 20 60.29 50.00 4.00 0.00 ACAAAAACGGAGAGCTGTGG PRODUCT SIZE: 180, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00 Statistics con too in in no tm tm high high high sid many tar excl bad GC too too any 3' poly end ered Ns get reg GC% clamp low high compl compl X stab ok Left 13950 0 0 0 4225 0 868 7765 0 1 146 104 841 Right 13836 0 0 0 4201 0 968 7447 0 1 146 121 952 Pair Stats: considered 613, unacceptable product size 595, high end compl 2, ok 16 primer3 release 1.1.4 (primer3_results.cgi release 0.4.0)

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