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Chromatography

This document discusses different types of chromatography techniques. It describes chromatography as separating the components of a mixture due to differences in adsorption to a stationary phase and solubility in a mobile phase. It then lists and describes some common types of chromatography, including liquid-liquid, liquid-solid, gas-liquid, thin layer chromatography, and paper chromatography. Key steps in thin layer chromatography and paper chromatography are outlined.

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73 views

Chromatography

This document discusses different types of chromatography techniques. It describes chromatography as separating the components of a mixture due to differences in adsorption to a stationary phase and solubility in a mobile phase. It then lists and describes some common types of chromatography, including liquid-liquid, liquid-solid, gas-liquid, thin layer chromatography, and paper chromatography. Key steps in thin layer chromatography and paper chromatography are outlined.

Uploaded by

liapriela
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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CHROMATOGRAPHY

separation of the components of the mixture due to differences adsorption by the stationary phase and solubility in the mobile phase.

TYPES OF CHROMATOGRAPHY
Type Liquidliquid Liquid-solid examples Paper HPLC Column TLC Gas Mobile Phase Liquid solvent system Liquid solvent system High-boiling pt. liquid Stationary phase Adsorbed water on the surface of the paper Adsorbent material (silica gel) Inert gas

Gas-Liquid

Stationary Phase It is fixed in place; either in a column or a planar surface Mobile Phase is a solvent or solvent system used to carry the components of a mixture through a stationary phase polarity of the eluent/solvent affects the relative rates at which compounds move through the column

THIN LAYER CHROMATOGRAPHY


Steps: 1. 2. 3. 4. Sample Loading/ Application Development of Chromatogram Visualization Documentation and Evaluation

PAPER CHROMATOGRAPHY

Step 2: Development of Chromatogram - solvent rises by capillary action up through the adsorbent - differential partitioning occurs between the components of the mixture dissolved in the solvent and the stationary adsorbent phase CHROMATOGRAM pattern formed by substances that have been separated by chromatography output or result of the chromatographic technique Step 4: Documentation and Interpretation of Data

Rf value
- is characteristic for any given compound on the same stationary phase using the same mobile phase for development of the plates - it depends on the following parameters: solvent system adsorbent amount of material spotted temperature

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