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A Study On Antifungal Activity of Actinobacteria Isolated From Soil Samples

The study isolated 30 actinobacteria strains from soil samples in Tamil Nadu, India. 11 of these strains showed antifungal activity against Candida albicans and Geotrichum candidum. The isolates were screened on starch casein agar to identify 20 actinobacteria species. Secondary screening found strains A7, A8, A23, C2, F3, and F4 produced the largest inhibition zones. Crude extracts from these strains using hexane and ethyl acetate showed inhibitory activity. Thin layer chromatography of strains F3 and F4 identified compounds with Rf values of 0.82 and 0.88 respectively.

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50 views

A Study On Antifungal Activity of Actinobacteria Isolated From Soil Samples

The study isolated 30 actinobacteria strains from soil samples in Tamil Nadu, India. 11 of these strains showed antifungal activity against Candida albicans and Geotrichum candidum. The isolates were screened on starch casein agar to identify 20 actinobacteria species. Secondary screening found strains A7, A8, A23, C2, F3, and F4 produced the largest inhibition zones. Crude extracts from these strains using hexane and ethyl acetate showed inhibitory activity. Thin layer chromatography of strains F3 and F4 identified compounds with Rf values of 0.82 and 0.88 respectively.

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RAMAKRISHNAN
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© © All Rights Reserved
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PUBLICATIONS

A PAPER COMMUNICATED OTHER THAN RELATED TO THESIS



A STUDY ON ANTIFUNGAL ACTIVITY OF
ACTINOBACTERIA ISOLATED FROM SOIL SAMPLES

U.Priya, P.Palanivel
Department of Microbiology, Vivekanandha College of Arts and Sciences for Women,
Elayampalayam- 637205, Tiruchengode, Namakkal District, Tamilnadu (India).
e.mail: [email protected]

ABSTRACT:
The endophytic actinomycetes can producing many antifungal compounds that can
suppress growth of pathogenic fungi. A total 30 actinomycetes strains were isolated from
Erode, Namakkal, Salem, Rameshwaram, Tiruchengode. They were screened for their
antifungal activity. Out of the 30 strains, 11 showed antifungal activity. Actinobacteria were
isolated by serial dilution and plating method on starch casein agar media. Total 20 species
were isolated on the bases of colony characteristics on starch casein agar and all the isolates
were primarily screened for antifungal activity of the test organisms Candida albicans and
Geotrichum candidum. In secondary screening (A7,A8,A23,C2,F3,F4) showed the maximum
zone of inhibition. Bioactive compound of the crude extractHexane 5:1(v/v) Ethyl acetate
1:1(v/v) are more inhibitory activity. Thin layer chromatography the solvent front with the
Rf value of 0.82 for F3 strains and 0.88 for F4 strains.
Keywords: Actinobacteria, Antibacterial activity, starch casein agar

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