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CH4303-Bioseparation Tutorial 3

Alpha (α) is a dimensionless number used in chromatography to describe how selectively a solute distributes between the stationary and mobile phases. It is defined as the ratio of the time a solute spends in the mobile phase to the time it spends in the stationary phase during the chromatographic process. Alpha values typically range from 0 to 1. An α value close to 0 indicates that the solute spends most of its time in the stationary phase and elutes later. An α value close to 1 means the solute spends very little time interacting with the stationary phase and elutes earlier. Alpha is useful for comparing how strongly different solutes interact with the stationary phase during chromatography.

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33 views

CH4303-Bioseparation Tutorial 3

Alpha (α) is a dimensionless number used in chromatography to describe how selectively a solute distributes between the stationary and mobile phases. It is defined as the ratio of the time a solute spends in the mobile phase to the time it spends in the stationary phase during the chromatographic process. Alpha values typically range from 0 to 1. An α value close to 0 indicates that the solute spends most of its time in the stationary phase and elutes later. An α value close to 1 means the solute spends very little time interacting with the stationary phase and elutes earlier. Alpha is useful for comparing how strongly different solutes interact with the stationary phase during chromatography.

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WeimingTan
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© © All Rights Reserved
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Download as PDF, TXT or read online on Scribd
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CH4303-Bioseparation Tutorial 3

Question: What is the value of alpha?

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