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Tube Marking Dilution Volume of Dilution Inoculated Into LST Broth Tubes Amount of Product Represented Per Tube

This document outlines a tube marking and dilution scheme for testing different amounts of product represented in liquid or broth samples. It shows that undiluted samples and serial dilutions from 10-1 to 10-4 are inoculated into broth tubes in volumes of 1mL, with the amount of product represented in each tube decreasing with more dilute samples from 1g to 0.0001g.

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Abubakker Siddiq
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23 views

Tube Marking Dilution Volume of Dilution Inoculated Into LST Broth Tubes Amount of Product Represented Per Tube

This document outlines a tube marking and dilution scheme for testing different amounts of product represented in liquid or broth samples. It shows that undiluted samples and serial dilutions from 10-1 to 10-4 are inoculated into broth tubes in volumes of 1mL, with the amount of product represented in each tube decreasing with more dilute samples from 1g to 0.0001g.

Uploaded by

Abubakker Siddiq
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Tube

Marking*

Dilution

Volume of dilution inoculated into LST


broth tubes

Amount of product
represented per tube

undil. 100

1 mL of undiluted liquids into 10 mL single


strength medium

1 mL

undil. 100

10 mL of 10-1 dilution of solids into 10 mL of


double strength medium

1g

1:10 10-1

1 mL of 10-1 dilution into 10 mL single


strength medium

0.1 g or mL

1:100 10-2

1 mL of 10-2 dilution into 10 mL single


strength medium

0.01 g or mL

1:1000 10-

1 mL of 10-3 dilution into 10 mL single


strength medium

0.001 g or mL

1:10000
10-4

1 mL of 10-4 dilution into 10 mL single


strength medium

0.0001 g or mL

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