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Continuous ExtracaSDtion of Fatty Acids or Their Conversion To Something Non

The document discusses various methods for the continuous extraction of fatty acids from cells or their conversion to non-toxic compounds, including transporting long-chain fatty acids out of E. coli cells. It references studies on fatty acid transport proteins, the role of the fadL gene product in transporting long-chain fatty acids in E. coli, and MsbA transporter-dependent lipid A dephosphorylation. The expression of an acyl-ACP thioesterase cDNA in an E. coli fatty acid degradation mutant resulted in the secretion of free fatty acids into the medium. Various options are discussed for modifying fatty acids to be recognized and leave the cell or tricking transport mechanisms, and exporting to a lipid bil

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Jonathan Oswald
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23 views

Continuous ExtracaSDtion of Fatty Acids or Their Conversion To Something Non

The document discusses various methods for the continuous extraction of fatty acids from cells or their conversion to non-toxic compounds, including transporting long-chain fatty acids out of E. coli cells. It references studies on fatty acid transport proteins, the role of the fadL gene product in transporting long-chain fatty acids in E. coli, and MsbA transporter-dependent lipid A dephosphorylation. The expression of an acyl-ACP thioesterase cDNA in an E. coli fatty acid degradation mutant resulted in the secretion of free fatty acids into the medium. Various options are discussed for modifying fatty acids to be recognized and leave the cell or tricking transport mechanisms, and exporting to a lipid bil

Uploaded by

Jonathan Oswald
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Continuous extraction of fatty acids or their

conversion to something non-toxic.

http://link.springer.com/article/10.1385%2FJMN
%3A16%3A2-3%3A99
http://www.plefa.com/article/S0952-3278(10)000736/abstract

http://www.microbialcellfactories.com/content/11/1/41

1. Stahl A, Gimeno RE, Tartaglia LA, Lodish HF: Fatty acid transport proteins: a current view of a
growing family.

Trends Endocrinol Metab 2001, 12(6):266-273. PubMed Abstract | Publisher Full Text

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2. Nunn WD, Colburn RW, Black PN: Transport of long-chain fatty acids in Escherichia coli.
Evidence for role of fadL gene product as long-chain fatty acid receptor.

J Biol Chem 1986, 261(1):167-171. PubMed Abstract | Publisher Full Text

3. Wang X, Karbarz MJ, McGrath SC, Cotter RJ, Raetz CRH: MsbA Transporter-dependent Lipid A 1Dephosphorylation on the Periplasmic Surface of the Inner Membrane.

J Biol Chem 2004, 279(47):49470-49478. PubMed Abstract | Publisher Full Text |


PubMed Central Full Text

The expression of full-length acyl-ACP thioesterase cDNA from Umbellularia californica in the E. coli fatty
-

acid-degradation mutant strain (fadD ) resulted in the secretion of free fatty acid into medium [9].

http://www.ncbi.nlm.nih.gov/pubmed/17901542
transport in
Modify fatty acids to be recognized to leave cell? Trick
the cell?
Export to lipid bilayer

http://www.ncbi.nlm.nih.gov/pubmed/11278265
http://www.jbc.org/content/279/43/45102.long

Or do we want to engineer a method for phospholipid


production for biofuel?

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