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Assay Interferences

This document lists potential interferences for various clinical analytes that could impact test results. It notes that hemolysis, lipids, and bilirubin can interfere with several analytes on a semi-quantitative basis. Other issues mentioned include specific preanalytical variables like sample handling and processing delays. The document concludes by stating that heterophile antibodies and rheumatoid factors can affect most immunoassays and outlines best practices to avoid preanalytical errors.

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17 views

Assay Interferences

This document lists potential interferences for various clinical analytes that could impact test results. It notes that hemolysis, lipids, and bilirubin can interfere with several analytes on a semi-quantitative basis. Other issues mentioned include specific preanalytical variables like sample handling and processing delays. The document concludes by stating that heterophile antibodies and rheumatoid factors can affect most immunoassays and outlines best practices to avoid preanalytical errors.

Uploaded by

dr_4u
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
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SPECIFIC TEST INTERFERENCES

Analyte

Interference (Semiquantitative)
Haemolysis

Albumin
ALP
Amylase - total

Bilirubin

Lipids

Other (Not quantitative)


High serum proteins; venous stasis at
collection.
Ascorbate
Macroamylasaemia; salivary amylase
component. (Usually done on fluids only).

AST
.
Bilirubin - conj.

Avoid direct sunlight

Bilirubin - total

Avoid direct sunlight.

Bilirubin - neonatal

Avoid direct sunlight

Calcium - ionised

Calcium - total

Not reported if pH < 7.2 or > 7.7;


use only designated blood gas syringe;
do not use heparin to fill dead space of
syringe; aerobic collections.
Citrated blood products, high protein

Chloride
Cholesterol - HDL

Not reported if triglycerides > 10

Cholesterol - LDL

Calculated value, not reported if


triglycerides > 5.0

Cholesterol - total

Ascorbate
(Reporting of lipid values subject to change)

CK

Macro-CK

Creatinine

Acetoacetate; cephalosporins;
frusemide; glucose; protein; drugs.

CRP - urgent
Glucose
Iron

Delayed processing if not in fluoride


tube; high WBC
Iron therapy

Analyte

Interference (Semiquantitative)
Haemolysis

LD

Bilirubin

Lipid

Other (Not quantitative)


Macro-LD

Mg++
O2 sat % Estimated
PCO2

Carboxy-, met-, sulfhaemoglobins; genetic


haemoglobin variants
Air bubble

pH

Air bubble

PO2

Air bubble

Potassium

Severe leucocytosis, fist clenching


with tourniquet; delayed processing.

PO4
ProBNP

Renal failure minor increase only.

Protein total

Venous stasis at collection.

Sodium

cTNI

Electrolyte exclusion effect of high


serum triglycerides or protein; delay in
processing.
Heterophile antibodies

cTNT

Renal failure, heterophile antibodies.

.Triglyceride

Ascorbate, glycerol.

Urate

Glucose; ascorbate; paracetamol

Not usually affected: lactate, GGT, ALT, lipase, pancreatic amylase, urea.
Heterophile antibodies and rheumatoid factors can affect most immunoassays.
Avoid contamination with incorrect anticoagulant, taking blood from drip arm, delayed separation.

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