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Precaution Step Gram Stain Method

Young bacterial cultures should be used for the Gram stain method to accurately identify Gram-positive and Gram-negative bacteria. The amount of alcohol applied during differential staining must be carefully controlled so as not to over-decolorize the sample and incorrectly identify bacteria. An even, thin smear is also important to avoid uneven decolorization from the alcohol treatment.

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Solah Ibnu Musa
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18K views

Precaution Step Gram Stain Method

Young bacterial cultures should be used for the Gram stain method to accurately identify Gram-positive and Gram-negative bacteria. The amount of alcohol applied during differential staining must be carefully controlled so as not to over-decolorize the sample and incorrectly identify bacteria. An even, thin smear is also important to avoid uneven decolorization from the alcohol treatment.

Uploaded by

Solah Ibnu Musa
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Precaution step gram stain method

Always use a young culture because older cultures of Gram-positive


bacteria tend to lose the ability to retain the crystal violet-iodine
complex and appear to be Gram-negative; but some bacteria are
naturally only weakly Gram-positive.
The amount of alcohol treatment (the differential stage) must be
judged carefully because over-treatment washes the crystal violetiodine complex from Gram-positive bacteria and they will appear to
be Gram-negative.
Take care to make an even smear otherwise alcohol will continue to
wash the violet/purple colour from thick parts of the smear while
thin parts are being over-decolorised.
At the end of the procedure, check that the labeling has not been
washed off by the alcohol.
Dont despair if the stained smear is not visible to the naked eye;
this may happen with a Gram-negative reaction.
Specimen stained slides are available from suppliers.

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