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Expo Bio Gompertz

This document contains data from experiments on the growth of sanguaza (a type of algae) under different pH and concentration conditions. It includes tables with population values (N) over time, as well as calculated values like generation time (TG), maximum growth rate (Umax), and parameters (λ, a, b, c) from fitted models of the form Y=a*exp(-exp(b-c*T)). Regression analyses were performed to develop models relating the phase lag (λ) and maximum growth rate (Umax) to pH and concentration. The models found phase lag to increase with lower pH and concentration, while maximum growth rate decreased with lower pH and increased concentration.

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Eduardo Paredes Chero
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40 views

Expo Bio Gompertz

This document contains data from experiments on the growth of sanguaza (a type of algae) under different pH and concentration conditions. It includes tables with population values (N) over time, as well as calculated values like generation time (TG), maximum growth rate (Umax), and parameters (λ, a, b, c) from fitted models of the form Y=a*exp(-exp(b-c*T)). Regression analyses were performed to develop models relating the phase lag (λ) and maximum growth rate (Umax) to pH and concentration. The models found phase lag to increase with lower pH and concentration, while maximum growth rate decreased with lower pH and increased concentration.

Uploaded by

Eduardo Paredes Chero
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as XLSX, PDF, TXT or read online on Scribd
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DETERMINACION POR DCCR

Numero de factores (n)


Valor alfa ()

sanguaza
pH

2
1.41421356

0 .7
0 .6
0 .5
0 .4
0 .3
0 .2
0 .1
C :1

0 .0
-0 .1
-0 .2

-2 0

M o d e l: Y A = a * e x p ( - e x p ( b - c * T A ) )
y = (,6 1 0 8 5 7 )* e x p (-e x p ((2 ,7 2 3 3 3 )-(,0 3 0 3 9 5 )* x ))
0 .7
0 .6
C :9

0 .5
C :8
0 .4
C :7
0 .3
C :6
0 .2

C :5
C :4

0 .1
C :1

0 .0

C :3
-0 .1
-0 .2

C :2
-2 0

20

40

60

80

100
T A

120

140

e xp (b -c *T A ))
2 3 3 3 )-(,0 3 0 3 9 5 )* x ))
C :1 0

C :1 2
C :1 1

C :9
C :8

C :7

120

140

160

180

200

220

Tiempo (h)
0
36
48
60
72
96
108
120
144
168
192
204
216
228
240

N1
350000
250000
300000
450000
500000
600000
750000
950000
1050000
1400000
1250000
1400000

Log (N/No)
0.0000
-0.1461
-0.0669
0.1091
0.1549
0.2341
0.3310
0.4337
0.4771
0.6021
0.5528
0.6021

N2
350000
300000
300000
400000
500000
700000
800000
1050000
1200000
1250000
1250000
1300000
1300000

Log (N/No)
0.0000
-0.0669
-0.0669
0.0580
0.1549
0.3010
0.3590
0.4771
0.5351
0.5528
0.5528
0.5699
0.5699

Ensayos

pH

Concentracion
sanguaza (%)

Umax

TG

1
2
3
4
5
6
7
8
9
10
11
12

7.29
7.29
8.71
8.71
7
9
8
8
8
8
8
8

2.44
4.56
2.44
4.56
3.5
3.5
2
5
3.5
3.5
3.5
3.5

0.019
0.023
0.025
0.025
0.022
0.020
0.028
0.025
0.037
0.052
0.043
0.048

37.33234
30.67783
28.23738
28.28978
31.57349
34.82143
24.85929
27.91106
18.64118
13.21401
16.11271
14.41845

Fase lag ()
Coeficiente
P
0.004
Intercepcin 1254.801
Parametro
(1) pH (L)
pH (Q)
(2) CS (L)
CS (Q)
1L by 2L

-286.390
16.630
-18.837
-0.550
3.061

0.004
0.005
0.339
0.619
0.202

Velocidad de crecimiento (Umax)


Coeficiente
P
-1.68944
0.018
0.40177
0.018
-0.02476
0.018
0.07091
0.164
-0.00859
0.034
-0.00135
0.777

FASE LAG
Mean/Interc.

Regressn
Coeff.
1254.801

Std.Err. Pure Err

t(3)

154.4741

8.1231

0.003900

(1)pH
(L)
pH
(Q)
(2)CS
(L)
CS
(Q)
1L by 2L
LAG=

-286.390
16.630
-18.837
-0.550
3.061

36.3297
2.2314
16.5891
0.9936
1.8789

-7.8831
7.4530
-1.1355
-0.5531
1.6291

0.004254
0.005001
0.338683
0.618768
0.201782

1254.801-286.390*pH+16.630*pH*pH-18.837*C-0.550*C*C+3.061*pH
VELOCIDAD DE CRECIMIENTO (Umax)

Mean/Interc.
(1)pH
(L)
pH
(Q)
(2)CS
(L)
CS
(Q)
1L by 2L

Regressn
Coeff.

Std.Err. Pure Err

t(3)

-1.68944
0.40177
-0.02476
0.07091
-0.00859
-0.00135

0.359447
0.084536
0.005192
0.038601
0.002312
0.004372

-4.70012
4.75262
-4.76858
1.83695
-3.71501
-0.30946

0.018220
0.017679
0.017518
0.163534
0.033924
0.777222

N3
350000
300000
650000
700000
700000
1050000
1050000
1150000
1400000
1600000
1300000
1050000

Log (N/No)
0.0000
-0.0669
0.2688
0.3010
0.3010
0.4771
0.4771
0.5166
0.6021
0.6601
0.5699
0.4771

N4
350000
250000
400000
600000
800000
850000
1100000
1250000
1600000
1500000
1550000
1550000
1550000
1800000

Log (N/No)
0.0000
-0.1461
0.0580
0.2341
0.3590
0.3854
0.4973
0.5528
0.6601
0.6320
0.6463
0.6463
0.6463
0.7112

56.70
57.89
30.73
41.13
64.04
55.85
40.74
43.37
37.14
43.82
42.07
41.21

0.61
0.57
0.57
0.66
0.556
0.644
0.53
0.58
0.644
0.62
0.63
0.62

2.72
3.29
2.33
2.53
3.529
2.727
3.16
2.87
3.145
4.69
3.89
4.20

0.03
0.04
0.04
0.04
0.039
0.031
0.05
0.04
0.058
0.08
0.07
0.08

Tiempo de generacion (TG)


Crecimiento maximo (a)
Coeficiente
P
Coeficiente
P
1318.570
0.002
0.250892
0.695
-300.937
0.002
0.117959
0.451
18.245
0.002
-0.015138
0.169
-53.642
0.030
-0.136124
0.117
5.078
0.009
-0.028594
0.005
2.228
0.249
0.043912
0.008

TIEMPO DE GENERACION (TG)


-95,% Cnf.Limt

+95,% Cnf.Limt

763.195

1746.406

Mean/Interc.

-402.007
9.529
-71.631
-3.712
-2.919

-170.772
23.732
33.957
2.613
9.040

(1)pH
(L)
pH
(Q)
(2)CS
(L)
CS
(Q)
1L by 2L

C-0.550*C*C+3.061*pH*C
POBLACION MAXIMA (a)
-95,% Cnf.Limt

+95,% Cnf.Limt

-2.83336
0.13274
-0.04128
-0.05194
-0.01595
-0.01527

-0.545524
0.670798
-0.008235
0.193755
-0.001231
0.012561

Mean/Interc.
(1)pH
(L)
pH
(Q)
(2)CS
(L)
CS
(Q)
1L by 2L

N5
350000
250000

Log (N/No)
0.0000
-0.1461

350000
450000
600000
800000
850000
1250000
1100000

0.0000
0.1091
0.2341
0.3590
0.3854
0.5528
0.4973

N6
Log (N/No)
350000
0.0000
300000
-0.0669
400000
450000
700000
950000
900000
1150000
1200000
1550000
1550000
1550000
1500000

0.0580
0.1091
0.3010
0.4337
0.4102
0.5166
0.5351
0.6463
0.6463
0.6463
0.6320

Umax

0.973
0.991
0.950
0.974
0.969
0.991
0.985
0.974
0.986
0.983
0.980

56.70
57.89
30.88
41.13
66.55
62.16
40.74
43.37
39.11
43.82
42.07
40.41

0.019
0.023
0.025
0.025
0.024
0.023
0.028
0.025
0.043
0.052
0.043
0.044

Std.Err. Pure Err

t(3)

128.4876

10.26224

0.001973

0.982

Regressn
Coeff.
1318.570

N7
Log (N/No)
350000
0.0000
300000
-0.0669
450000
0.1091
550000
0.1963
750000
0.3310
1000000
0.4559
950000
0.4337
1050000
0.4771
1300000
0.5699
1200000
0.5351

TG
37.33
30.68
27.98
28.29
29.4
29.93
24.86
27.91
16.24
13.21
16.11
15.59

a
0.61
0.57
0.56
0.66
0.55
0.63
0.53
0.58
0.62
0.62
0.63
0.64

-95,% Cnf.Limt +95,% Cnf.Limt


909.665

1727.475

-300.937
18.245
-53.642
5.078
2.228

30.2181
1.8560
13.7984
0.8265
1.5628

-9.95881
9.83028
-3.88753
6.14492
1.42558

0.002154
0.002238
0.030171
0.008669
0.249227

Regressn
Coeff.

Std.Err. Pure Err

t(3)

0.250892
0.117959
-0.015138
-0.136124
-0.028594
0.043912

0.580772
0.136588
0.008389
0.062370
0.003736
0.007064

0.43200
0.86362
-1.80450
-2.18253
-7.65456
6.21627

0.694910
0.451320
0.168917
0.117071
0.004631
0.008391

-397.104
12.338
-97.554
2.448
-2.746

-204.769
24.151
-9.729
7.709
7.201

-95,% Cnf.Limt +95,% Cnf.Limt


-1.59738
-0.31672
-0.04184
-0.33461
-0.04048
0.02143

2.099169
0.552643
0.011560
0.062364
-0.016706
0.066393

N8
Log (N/No)
350000
0.0000
300000
-0.0669
400000
0.0580
500000
0.1549
700000
0.3010
900000
0.4102
1050000
0.4771
1250000
0.5528
1100000
0.4973
1150000
0.5166
1050000
0.4771
1250000
0.5528
1650000
0.6734
1500000
0.6320
1500000
0.6320

N9
Log (N/No)
350000
0.0000
350000
0.0000
450000
0.1091
850000
0.3854
1000000
0.4559
1150000
0.5166
1300000
0.5699
1450000
0.6173
1450000
0.6173
1600000
0.6601
1800000
0.7112
1650000
0.6734

N10
Log (N/No)
350000
0.0000
300000
-0.0669
400000
0.0580
800000
0.3590
1050000
0.4771
1100000
0.4973
1350000
0.5863
1400000
0.6021
1400000
0.6021
1650000
0.6734
1700000
0.6864
1600000
0.6601
1500000
0.6320

Regressn Coeff.
Mean/Interc.
(1)PH
(L)
PH
(Q)
(2)CC
(L)
CC
(Q)
1L by 2L

1254.801
-286.390
16.630
-18.837
-0.550
3.061

SS
(1)PH
PH

(L)
(Q)

369.478
444.279

(2)CC
(L)
CC
(Q)
1L by 2L
Lack of Fit
Pure Error

29.266
2.446
21.227
167.927
23.995

N11
Log (N/No)
350000
0.0000
300000
-0.0669
400000
0.0580
800000
0.3590
950000
0.4337
1100000
0.4973
1300000
0.5699
1450000
0.6173
1400000
0.6021
1600000
0.6601
1750000
0.6990
1700000
0.6864
1350000
0.5863

Std.Err. Pure Err


154.4741
36.3297
2.2314
16.5891
0.9936
1.8789

t(3)

-95.% Cnf.Limt +95.% Cnf.Limt

0.003900
0.004254
0.005001
0.338683
0.618768
0.201782

763.195
-402.007
9.529
-71.631
-3.712
-2.919

MS

369.4782

46.19523

0.006512

444.2787

55.54741

0.005001

df

8.12305
-7.88307
7.45301
-1.13548
-0.55306
1.62909

N12
Log (N/No)
350000
0.0000
300000
-0.0669
450000
0.1091
850000
0.3854
1000000
0.4559
1150000
0.5166
1350000
0.5863
1450000
0.6173
1450000
0.6173
1350000
0.5863
1800000
0.7112
1600000
0.6601

1746.406
-170.772
23.732
33.957
2.613
9.040

1
1
1
3
3

29.2663
2.4464
21.2267
55.9757
7.9982

3.65911
0.30587
2.65393
6.99855

0.151696
0.618768
0.201782
0.072166

ANOVA Fase Lag


(1)pH
(L)
pH
(Q)
(2)CS
(L)
CS
(Q)
1L by 2L
Lack of Fit
Pure Error
Total SS

Modelo
Residuos
Total
Rcuad
Error

SS

df

MS

369.478
439.288
29.266
2.831
21.227
167.921
21.158
1083.835

1
1
1
1
1
3
3
11

369.4782
439.2876
29.2663
2.8312
21.2267
55.9738
7.0528

52.38742
62.28552
4.14959
0.40142
3.00968
7.93638

SS

df

MS

1062.7

8
3
11

132.83
7.05

21.2
1083.8

0.9804782
0.0195218

18.83
MAYOR

98.0%
2.0%

ANOVA TG

(1)pH
(L)
pH
(Q)
(2)CS
(L)
CS
(Q)
1L by 2L
Lack of Fit
Pure Error
Total SS

Modelo
Residuos
Total
Rcuad
Error

SS

df

MS

369.478
444.279
29.266
2.831
21.227
167.921
21.158
1083.835

1
1
1
1
1
3
3
11

369.4782
439.2876
29.2663
2.8312
21.2267
55.9738
7.0528

52.38742
62.28552
4.14959
0.40142
3.00968
7.93638

SS

df

MS

1062.7

8
3
11

132.83
7.05

21.2
1083.8

0.9804782
0.0195218

98.0%
2.0%

18.83
MAYOR

ANOVA Umax
p
0.005440
0.004240
0.134427
0.571360
0.181178
0.061371

(1)pH
(L)
pH
(Q)
(2)CS
(L)
CS
(Q)
1L by 2L
Lack of Fit
Pure Error
Total SS

F tabla

8.85
MAYOR

Modelo
Residuos
Total
Rcuad
Error

p
0.005440
0.004240
0.134427
0.571360
0.181178
0.061371

F tabla

8.85
MAYOR

SS

df

MS

0.0000
0.0010
0.0000
0.0006
0.0000
0.0000
0.0001
0.0015

1.0000
1.0000
1.0000
1.0000
1.0000
3.0000
3.0000
11.0000

0.0000
0.0010
0.0000
0.0006
0.0000
0.0000
0.0000

SS

df

MS

0.0014
0.0001
0.0015

8.0000
3.0000
11.0000

0.0002
0.0000

0.92346882
0.07653118

92.3%
7.7%

0.0818
26.1204
0.0004
15.9387
0.1079
0.2178

0.7935
0.0145
0.9861
0.0281
0.7641
0.8787

F tabla

4.5250
8.8452
MAYOR

F itt e d S u r fa c e ; V a r ia b le : a
2 fa c to r s , 1 B lo c k s , 1 2 R u n s ; M S R e s id u a l= ,0 0 0 4
D V: a

O b s e rv e d v s . P re d ic t e d V a lu e s
2 fa c t o r s , 1 B lo c k s , 1 2 R u n s ; M S R e s id u a l= 3 1 , 9 8 6 9 4
D V : F ase LA G
75
70

P redic ted V alues

65
60
55
50
45
40
35
30

25

30

35

40

45

50

O b s e rve d V a lu e s

55

60

65

; V a r ia b le : a
s ; M S R e s id u a l= , 0 0 0 4 4 3 7
a

0
0
0
0
0
0

,6 5
,6
,5 5
,5
,4 5
,4

es
u a l= 3 1 , 9 8 6 9 4

O b s e rve
2 fa c t o rs , 1 B lo c k s
0 ,6 8
0 ,6 6

P redic ted V alues

0 ,6 4
0 ,6 2
0 ,6 0
0 ,5 8
0 ,5 6
0 ,5 4

55

60

65

70

0 ,5 2
0 ,5 0

0 ,5 2

0 ,5 4

0 ,5 6

F itte d S u r fa c e ; V a r ia b le : a
2 fa c to r s , 1 B lo c k s , 1 2 R u n s ; M S R e s id u a l= ,0 0 0 4 4 3 7
D V: a
5 ,5
5 ,0
4 ,5

CS

4 ,0
3 ,5
3 ,0
2 ,5
2 ,0
1 ,5
6 ,8

7 ,0

7 ,2

7 ,4

7 ,6

7 ,8

8 ,0

8 ,2

8 ,4

8 ,6

p H

O b s e rv e d v s . P r e d ic t e d V a lu e s
2 fa c t o r s , 1 B lo c k s , 1 2 R u n s ; M S R e s id u a l= , 0 0 0 4 4 3 7
D V : a

0 ,5 2

0 ,5 4

0 ,5 6

0 ,5 8

0 ,6 0

O b s e rve d V a lu e s

0 ,6 2

0 ,6 4

0 ,6 6

0 ,6 8

8 ,8

9 ,0

9 ,2

0
0
0
0
0
0

,6
,6
,5
,5
,4
,4

M od
y = (,6 5 9 7 2 9
0 ,8
4 3 7

0 ,7
0 ,6
0 ,5
0 ,4

C :

0 ,3

C :4

0 ,2
0 ,1

8 ,6

8 ,8

9 ,0

9 ,2

0
0
0
0
0
0

,6 5
,6
,5 5
,5
,4 5
,4

C :3
C :1

0 ,0
-0 ,1
-0 ,2

C :2
-2 0

20

40

60

M o d e l: Y A = a * e x p ( - e x p ( b - c * T A ) )
y = ( ,6 5 9 7 2 9 )* e x p (- e x p ((2 ,5 2 7 5 9 ) -(,0 3 7 1 3 9 )* x ))
C :1 4
C :9

C :1 1 C :1 2 C :1 3

C :1 0

C :8
C :7
C :6

C :5
C :4

C :3

C :2
20

40

60

80

100

120
T A

140

160

180

200

220

240

C :1 4
C :1 3

220

240

ph_c

C_c
-1.00000
-1.00000
1.00000
1.00000
-1.41421
1.41421
0.00000
0.00000
0.00000
0.00000
0.00000
0.00000

-1.00000
1.00000
-1.00000
1.00000
0.00000
0.00000
-1.41421
1.41421
0.00000
0.00000
0.00000
0.00000

ph
c
7.29289322
7.29289322
8.70710678
8.70710678
7
9
8
8
8
8
8
8

2.44
4.56
2.44
4.56
3.5
3.5
2
5
3.5
3.5
3.5
3.5

SS
(1)PH
(L)
PH
(Q)
(2)CC
(L)
CC
(Q)
1L by 2L
Lack of Fit
Pure Error
Total SS

369.478
444.279
29.266
2.446
21.227
167.927
23.995
1090.523

SS

MODELO
RESIDUO
TOTAL

R2
error totak

df

1064.082
26.441
1090.523

0.97575386
0.02424614 v

1
1
1
1
1
3
3
11

df

MS

369.4782
444.2787
29.2663
2.4464
21.2267
55.9757
7.9982

46.19523
55.54741
3.65911
0.30587
2.65393
6.99855

MS

7 152.011716 22.9963857
4 6.6102438
11

p
0.006512
0.005001
0.151696
0.618768
0.201782
0.072166

Ftabla

0.00444498 6.09421093

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