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Dna Structure Function and Replication Activity

1. DNA is a double-stranded molecule made up of nucleotides that contains genes which provide instructions for making proteins. Each cell needs different proteins to function properly. 2. Genes determine characteristics by influencing which proteins an organism makes. A person's DNA can determine if they have hemophilia based on variations in genes for blood clotting proteins. 3. DNA replication produces two identical copies of the original DNA before a cell divides, ensuring each daughter cell has the full set of genetic instructions.

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Dna Structure Function and Replication Activity

1. DNA is a double-stranded molecule made up of nucleotides that contains genes which provide instructions for making proteins. Each cell needs different proteins to function properly. 2. Genes determine characteristics by influencing which proteins an organism makes. A person's DNA can determine if they have hemophilia based on variations in genes for blood clotting proteins. 3. DNA replication produces two identical copies of the original DNA before a cell divides, ensuring each daughter cell has the full set of genetic instructions.

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Name __________________________________ Date __________________________ TOC #

_____
Regents Living Environment
Campbell

Ms.

Aim: DNA Structure, Function and Replication

DNA is a long molecule that consists of two strands of


nucleotides twisted together in a long spiral called a double
helix. DNA is made up of four different types of nucleotide:
A, C, G and T.

Each DNA molecule contains multiple genes. Each gene is a


segment of DNA with a sequence of nucleotides that
provides the instructions for making a protein.

A cell needs many different types of proteins to function.


For example, a cell needs:

protein enzymes to carry out the chemical reactions


needed for life

transport proteins to move ions and molecules into


and out of the cell and to move substances around
inside the cell

structural proteins.

1. All living organisms, including bacteria, plants, humans and other animals, are
made up of one or more cells. Explain why all living organisms need to have DNA.
Include the words genes and proteins in your explanation.

Genes influence an organism's characteristics by determining which types of

proteins the organism makes. The flowchart shows an example in humans. Two
different versions of a gene give the instructions for producing either a normal or
defective version of a protein enzyme which results in either normal skin and hair
color or albinism.
Gene in DNA

One version of the gene


provides instructions to
make
normal protein enzyme.
The other version of this
gene provides instructions
to make
defective enzyme.

Protein

Characteristic

Normal enzyme makes


the pigment molecule
in skin and hair.

Normal skin and


hair color

Defective enzyme does


not make this pigment
molecule.

Albinism (very
pale skin and hair)

2. For most people, when a blood vessel is injured the clotting proteins in their
blood produce a blood clot which prevents excessive bleeding. However, some
people have a defective version of one type of clotting protein, so clots do not form
normally. As a result, they tend to bleed excessively after even minor injuries; they
have hemophilia. Explain how a person's DNA can determine whether or not he has
hemophilia.

This drawing shows a short section of a DNA double helix with a diagram of four of
the nucleotides in each strand of the double helix. Each nucleotide has:

a phosphate group (P) and a sugar molecule in the backbone of the DNA
strand

one of the four bases (A = adenine, C = cytosine, G = guanine, or T =


thymine)

Each base in one strand of the DNA double helix pairs with a base in the other
strand of the double helix. The base-pairing rules describe which bases pair
together in a DNA double helix. Complete the following sentences to give the basepairing rules.
3. A in one strand always pairs with _____ in the other strand.
C in one strand always pairs with _____ in the other strand.
Since all the nucleotides in DNA are the same except for the base they contain,
each nucleotide is given the same symbol as the base it contains (A, C, G, or T).
A polymer consists of many repeats of a smaller
molecule (a monomer). For example, a protein is a
polymer of amino acids.

4. DNA is a polymer of _________________________ .


The sequence of nucleotides in a gene determines which amino acids are joined to
form a protein. Slight differences in the sequence of nucleotides in a gene can result
in different versions of the protein which in turn can result in different
characteristics.
The sequence of nucleotides in the DNA of a gene
determines the sequence of amino acids in a protein which
determines the structure and function of the protein which
influences the characteristics or traits of the organism.
5. Explain how a difference in the sequence of nucleotides in a
gene could result in one of these boys being albino and the other
boy having normal skin and hair color.

DNA Replication
Our bodies need to make new cells to grow or to replace
damaged cells. New cells are formed by cell division which
occurs when a cell divides into two daughter cells. Before a
cell can divide, the cell must make a copy of all its DNA; this is
called DNA replication.
6. Explain why DNA replication is needed before a cell divides
into two daughter cells.

During DNA replication, the two strands of the DNA


helix are separated and each old strand provides the
information needed to make a new matching strand.
Each nucleotide in the new strand is matched to a
nucleotide in the old strand using the base-pairing
rules.
The enzyme DNA polymerase helps to make the new
matching DNA strand by adding the matching
nucleotides one at a time and joining each new
nucleotide to the previous nucleotide in the growing
DNA strand.
DNA replication results in two new DNA molecules
that are identical to the original DNA molecule. Thus,
each of the new DNA molecules carries the same
genetic information as the original DNA molecule.

This drawing shows a


short segment of DNA
which separates into two
strands in preparation for
replication.
Your job is to play the
role of DNA
polymerase and create
the new matching
strands of DNA to
produce two pieces of
double-stranded DNA.
Add matching
nucleotides one at a
time, using the basepairing rules and the
nucleotides and tape
provided by your
teacher.
7a. Are there any
differences between the
two double-stranded
pieces of DNA you have
made?
7b. Are these new
double-stranded pieces of
DNA the same as or
different from the original
piece of DNA?
8. Why is it important that both copies of the DNA molecule have the exact same
sequence of nucleotides as the original DNA molecule?

9. Based on the function of DNA polymerase, explain why each part of the name
DNA polymerase (DNA, polymer, -ase) makes sense.

10. Explain how DNA polymerase, the double helix structure of DNA, and the basepairing rules work together to produce two identical copies of the original DNA
molecule.

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