Determination of Bleeding Time
Determination of Bleeding Time
The Bleeding Time (BT) test was first described in 1901 by the French physician Milian.
Bleeding time is a crude test that has been used for the evaluation of platelet function during
formation of the primary hemostatic plug. It addresses how well platelets interact with blood
vessel walls to form blood clots following a wound or trauma. Hence, prolonged BT may
indicate a vascular defect, a platelet function defect or thrombocytopenia.
The classical 4 methods used for BT tests are Ivy, Template, Modified Template and Duke. The
normal range can vary depending on the method used but is typically between 2 and 9 minutes.
The major variables with regard to the methodology are venous pressure, position, direction and
depth of incision, skin temperature, and operator subjectivity.
The bleeding time test is usually used on patients who have a history of prolonged bleeding after
cuts or who have a family history of bleeding. This test is also performed as a preparatory test to
determine a patient’s bleeding response during and after injury.
Requirements:
1. Disposable needle
2. Rectified spirit
3. Cotton
4. Filter paper
5. Stopwatch
Method:
1. The tip of the finger was wiped with cotton soaked in alcohol (ring finger of the left
hand)
2. It was pricked with a disposable needle and let the drop ooze out without applying any
pressure.
3. Every 30 sec the blood drop was tapped on a strip of filter paper without touching the
skin.
4. The time from the appearance of the drop of blood to the time when bleeding stops and
the filter paper is no longer stained is taken as bleeding time.
Normal result:
Normal bleeding time is less than 5 min. Normal value for template method range upto 8
minutes while for the modified template methods upto 10 minutes.
Precautions:
1. After the experiment, all needle, cotton and papers should be discarded properly
2. Some medications adversely affect the results of bleeding time eg. Sulphonamise,
diuretics, anticoagulants, anticancer drugs, aspirin and non-steroidal anti-inflammatory
drugs.
3. Prolonged use of aspirin causes prolonged bleeding so it should not be taken 2 weeks
prior to the test.