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Module 3 Protein Trafficking

This document provides instructions for a tutorial on protein trafficking and targeting. It begins with a brief overview stating that proteins synthesized in the cytosol must be sorted and transported to their final destinations, such as organelles, using signal sequences encoded in the proteins. Students are directed to watch a video on early experiments in this area and complete a chart comparing protein targeting mechanisms to different organelles based on lecture information. This will aid in studying for an upcoming quiz on the topic.

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Jonathan Tsou
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279 views

Module 3 Protein Trafficking

This document provides instructions for a tutorial on protein trafficking and targeting. It begins with a brief overview stating that proteins synthesized in the cytosol must be sorted and transported to their final destinations, such as organelles, using signal sequences encoded in the proteins. Students are directed to watch a video on early experiments in this area and complete a chart comparing protein targeting mechanisms to different organelles based on lecture information. This will aid in studying for an upcoming quiz on the topic.

Uploaded by

Jonathan Tsou
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Bio/LS 2B03  Tutorial 3  Fall 2010 

Protein trafficking and targeting 
All proteins are synthesized from mRNAS in the cytosol. Those destined for secretion or for the 
various organelles of the cell must be sorted and transported to their final destination. This 
involves signals encoded into the proteins that will be recognized by the cellular machinery to 
transport the proteins to the correct locations. As was discussed in lecture there are specific 
signal sequences that target proteins to particular organelles and specificity can depend on 
both amino acid sequence and position within the peptide chain.  

For this tutorial you will need to: 

1. Watch the video presentation: just the first 6 slides describing the experiments of 
Jamieson and Palade, Blobel and Sabatini. 

http://hstalks.com/main/browse_talk_view.php?t=97&s=97&s_id=17&c=252  

2. Fill in the chart below, use it as a study tool for the quiz and subsequent exams. 
 
3. Complete Quiz 3: Protein targeting, posted on Avenue, due by 8:00am Saturday 
October 23rd (worth 4%) 

 
Bio/LS 2B03  Tutorial 3  Fall 2010 

Name:_______________________________ Student #:___________________ Tutorial section:_____ 

Use this chart to compare protein trafficking/transport to various organelles as you have learned in 
lecture.  Note that you should have enough details from lecture to complete the chart for ER, 
Mitochondria, and peroxisomes, but you will not be able to fully complete the chart for the organelles 
that were glossed over in class.  

  Peroxisome   Mitochondria  ER  Chloroplast  Nucleus 


Signal           
sequence(s)? 
What is it? 
 

Signal           
Sequence   
Receptor   
 
Translocation           
channels   
 
 
Energy           
source   
 
 
Targeting           
within   
organelle   
 
Co‐ or post‐           
translational?  
Is protein 
folded before 
transport? 
Defects lead           
to…   
 
 
 
 
Other notes…           
 
 
 
 
 

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