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6x DNA Loading Dye

The 6X Loading Dye & SDS Solution is recommended for preparing DNA samples containing high amounts of DNA binding proteins for gel electrophoresis. It contains SDS to denature proteins and release DNA from complexes. SDS also prevents DNA annealing and additional bands. The dye solution contains bromophenol blue, xylene cyanol FF, glycerol, SDS, and EDTA to protect DNA from nucleases during electrophoresis. It is tested for sample preparation prior to agarose gel electrophoresis.

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281 views

6x DNA Loading Dye

The 6X Loading Dye & SDS Solution is recommended for preparing DNA samples containing high amounts of DNA binding proteins for gel electrophoresis. It contains SDS to denature proteins and release DNA from complexes. SDS also prevents DNA annealing and additional bands. The dye solution contains bromophenol blue, xylene cyanol FF, glycerol, SDS, and EDTA to protect DNA from nucleases during electrophoresis. It is tested for sample preparation prior to agarose gel electrophoresis.

Uploaded by

me_dayakar
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Description

The 6X Loading Dye & SDS Solution is recommended for


preparation of DNA samples with high amounts of DNA
binding proteins prior to loading on agarose and
polyacrylamide gels. It is supplemented with 1% SDS to
CERTIFICATE OF ANALYSIS
eliminate DNA-protein interactions. Proteins have to be
denatured to release DNA from DNA-protein complexes.
6X Loading Dye & SDS Otherwise, the DNA binding proteins may alter
electrophoretic DNA migration, cause DNA samples to
Solution stick to gel wells or band shifts. SDS also prevents the
annealing of DNA molecules via long cohesive ends and
#R1151 5x1ml helps to avoid additional bands in electrophoresis.
The optimized 6X Loading Dye & SDS Solution contains
two electrophoresis tracing dyes: bromophenol blue and
Lot: xylene cyanol FF. It also contains 100mM EDTA, that
binds divalent metal ions, inhibits metal dependent
enzymatic reactions and protects DNA from metal
Store at -20°C at room temperature or at +4°C for dependent nucleases, effectively inactivating enzymes
periods up to 12 months. and protecting DNA from nucleases.
For longer periods, store.

In total 5 vials.
Composition QUALITY CONTROL
0.03% bromophenol blue Tested for DNA sample preparation prior to agarose gel
0.03% xylene cyanol FF electrophoresis.
60% glycerol Quality authorized by: Jurgita Zilinskiene
1% SDS
100mM EDTA (pH 7.6, adjusted with Tris)

Usage Recommendations
! Add 1 volume of 6X Loading Dye & SDS Solution to 5
volumes of DNA sample.
# Mix well.
$ Heat at 65°C for 10 minutes.
% Chill on ice, spin down and load.

Note
• In 1% agarose gels bromophenol blue co-migrates PRODUCT USE LIMITATION.
This product is developed, designed and sold exclusively for research purposes and in vitro
with ~300 bp DNA, while xylene cyanol FF co-migrates use only. The product was not tested for use in diagnostics or for drug development, nor is it
with ~4000 bp DNA. suitable for administration to humans or animals.
Please refer to www.fermentas.com for Material Safety Data Sheet of the product.
• The prepared sample can be stored at -20°C. It can be
subjected and is viable for several freeze-thaw cycles.

(1) Revised 10.01.2006

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