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Biology Laboratory Experiment Report

1) The document describes a biology lab experiment to test for the presence of amylase, glucose, protein, and fat in various food materials. 2) Students tested sugar, rice, flour, egg white, and palm oil using Lugol's, Benedict's, and Biuret solutions to identify carbohydrates, glucose, and proteins. They also used paper to test if palm oil contained fat. 3) The results showed that sugar contained glucose, rice and flour contained carbohydrates, egg white contained protein, and palm oil contained fat.

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18K views

Biology Laboratory Experiment Report

1) The document describes a biology lab experiment to test for the presence of amylase, glucose, protein, and fat in various food materials. 2) Students tested sugar, rice, flour, egg white, and palm oil using Lugol's, Benedict's, and Biuret solutions to identify carbohydrates, glucose, and proteins. They also used paper to test if palm oil contained fat. 3) The results showed that sugar contained glucose, rice and flour contained carbohydrates, egg white contained protein, and palm oil contained fat.

Uploaded by

Pujangga Tanpa Inspirasi
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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XI Science 4

SMA N 1 Bojonegoro

BIOLOGY LABORATORY EXPERIMENT REPORT

“FOOD MATERIAL TEST”

Biology Teacher:
Mrs. Dra. Anik Silfi W.
Name Of Group
• Agi Akbar Subraja (01)
• Alif Robath Safiu Surur ( )
• Badrut Anggara Putra ( )
• Lingga Hageng K. S. ( )
• Muhammad Ramadhan ( )
• Rahmandhika Firdauzha Hary Hernandha (27)
Objective
• To test an amilum, glucose, protein, and fat
which contained in food material
• To distinguish about the solution for this
experiment, there is Lugol solution, Benedict
(Fehling A and B) solution, Biuret solution and
how to test the fat with paper.
Utensils
 Reaction tube / test-tube  Beaker Glass

 Test-tube pincers  Pipette


 Ethylated (spiritus) burner

 Grinder
Materials
 Sugar  Test Solution (Biuret,
Benedict, and Lugol)

 Rice (packet of rice / lontong)


 Flour
 Palm Oil

 Egg (white part / albumin) Lugol reagent

Biuret reagent
 Paper
 Result Table
Procedure
1. Glucose Test
> Make a sugar solution with the water
> Pour it into test-tube until ±2cm from base tube
> Then put ±10 drops Benedict reagent into the test-tube
> Heat it on the burner with non direct contact and wait
until it changes into different colour.
> Record the result into the table.
> And do the same thing with the other solution (don’t
burn it on the burner, except Benedict reagent)
Glucose Test
2. Carbohydrate Test
> Make a rice solution (grind it before make the
solution) and flour solution.
> Pour it into the different test-tube until ±2cm from
base tube
> Then put ±5 drops lugol reagent into the test-tube
> Shake it periodically and wait until it changes into
different colour.
> Record the result into the table
> And do the same thing with the other solution
(don’t burn it on the burner, except Benedict reagent)
3. Protein Test
> Pour an albumin into beaker glass with the water
until become a solution
> Put it into test-tube until ±2cm from base tube
> Then put ±5 drops Biuret reagent into the test-
tube
> Shake it periodically and wait until it change into
different colour
> Record the result into the table
> And do the same thing with the other solution
(don’t burn it on the burner, except Benedict
reagent)
4. Fat Test
> Drop a palm oil on the paper (all kinds of paper is
not a matter)
> Then wait until an oil penetrated in the paper
> Look at the paper surface, if the paper become
transparent so the reagent (palm oil) is included a
fat.

Palm Oil spot


Discussion Report
1. Result Table of Food Material Test
2. Discussion
Based on the table above, the sugar solution changed into brick-
red colour when it heat on the burner with non direct contact
with Benedict (Fehling A and B) reagent, so it consist of glucose.
Because, the glucose’s food material will changed into brick-red
colour when it heat with Benedict reagent.
Then, the carbohydrate (in this experiment: rice and flour)
changed into dark blue colour when it shake with lugol reagent.
Because the carbohydrate’s food material will changed into dark
blue colour when it mix with lugol reagent. Next, albumin
changed into violet colour after we shake it with Biuret reagent.
Because, an albumin’s food material will changed into violet
when it mix with Biuret reagent.
And then, the last, about the fat. The drop of palm oil changes
the paper into transparent phase, it proof that palm oil is
contained with fat. Because, fat’s food material will changes a
paper become to transparent phase.
Conclusion
• We know about the contain of some food materials when
we test it with some reagent, like Lugol for Carbohydrate,
Benedict for Glucose, and Biuret for Protein.
• The fat will changes the paper become to transparent
phase, so we can know it from look at the result of paper
phase.
• And based on the materials in this experiment, so we can
conclude that rice and flour is contained with
carbohydrate, sugar is contained with glucose, albumin
(white part of egg) is contained with protein, and palm oil
is contained with fat.

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