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Beetroot Membranes Practical

This document provides the method for an experiment investigating the effect of temperature on beetroot cell membranes. Beetroot discs are exposed to different water bath temperatures ranging from 30 to 80 degrees Celsius for one minute each. The discs are then placed in labeled test tubes with water and left for over 20 minutes. The absorbency of the water and released pigment in each test tube is then measured with a colorimeter to determine the effect of temperature on beetroot cell membrane permeability and pigment release.

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Dana Porter
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573 views

Beetroot Membranes Practical

This document provides the method for an experiment investigating the effect of temperature on beetroot cell membranes. Beetroot discs are exposed to different water bath temperatures ranging from 30 to 80 degrees Celsius for one minute each. The discs are then placed in labeled test tubes with water and left for over 20 minutes. The absorbency of the water and released pigment in each test tube is then measured with a colorimeter to determine the effect of temperature on beetroot cell membrane permeability and pigment release.

Uploaded by

Dana Porter
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
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Anglo European School, Ingatestone

The Effect of Temperature on Membranes The colour of Beetroot is due to the presence of a red pigment in the cell sap. Materials: beetroot cork borer white tile scalpel 2 x 250 ml beakers glass-marking pen graduated pipette (10 ml) thermometer 6 x test tubes and rack mounted needle stopwatch Bunsen burner, tripod and gauze colorimeter with blue filter

Method: 1. Using the cork borer, cut 3 or 4 cylinders from your beetroot. Place these cylinders on the tile and use a scalpel to cut them into discs about 3 mm thick. You will need 36 discs. 2. Place the discs in a beaker and wash them in cold water for at least 5 minutes. Either leave them under a running tap or fill and empty the beaker about 5 times during the washing process.

3. Label 6 test tubes, each with one of the following: 30, 40, 50, 60, 70, and 80. Using the graduated pipette place 6 ml of cold tap water in each one. 4. 4. When the beetroot discs have been washed, impale 6 of them on a mounted needle with a space between each disc. 5. Prepare a water bath by half-filling a beaker with water at 30C.

6. Using a stopwatch, place the mounted needle with the discs in the water bath for exactly 1 minute. At the end of this time, take the discs off the needle and drop them into the tube labelled 30. 7. Heat the water bath to 40C. Impale 6 more discs, immerse them in the water bath for 1 minute and then transfer them to the tube labelled 40. 8. Repeat the procedure for temperatures 50, 60, 70 and 80C, and leave all the test tubes for 20 minutes or more. Fill a clean cuvette with tap water this will be your blank. Select the blue filter on the colorimeter, and use the blank to zero the colorimeter.

9.

GHB 2004

Anglo European School, Ingatestone


10. Shake the tubes to ensure that any pigment is evenly distributed through the water. For each tube, fill a cuvette with the water/pigment mixture, and read the absorbancy of the mixture with the colorimeter.

cuvette

GHB 2004

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