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Practice Questions For 2011 MICR2000 Midsemester Quiz 1 16-8-11

1. The practice quiz covers multiple choice and short answer questions about microbes. 2. The multiple choice section tests knowledge of early life on Earth, differences between microbial populations and communities, contributions to microbiology, microbial structures, and characteristics of various microbes. 3. The short answer section requires listing Koch's postulates, describing Gram staining results, explaining how penicillins work, and naming properties of the 16S rRNA gene that make it useful for measuring diversity.

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Alex Huy Nguyen
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394 views3 pages

Practice Questions For 2011 MICR2000 Midsemester Quiz 1 16-8-11

1. The practice quiz covers multiple choice and short answer questions about microbes. 2. The multiple choice section tests knowledge of early life on Earth, differences between microbial populations and communities, contributions to microbiology, microbial structures, and characteristics of various microbes. 3. The short answer section requires listing Koch's postulates, describing Gram staining results, explaining how penicillins work, and naming properties of the 16S rRNA gene that make it useful for measuring diversity.

Uploaded by

Alex Huy Nguyen
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
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Practice Quiz 1 for MICR2000 (should take 30 minutes)

Section A. Multiple Choice Questions (1 mark each) 1. Microbial cells first appeared on earth A about 2 million years ago Bnearly 2 billion years ago (C) nearly 4 billion years ago D after viruses Eafter the dinosaurs A microbial community differs from a microbial population in that A a community uses quorum sensing Ba community is derived from a single cell C a community can develop antibiotic resistance more rapidly D a community consists of interacting populations Ea community consists of multiple habitats Who first proposed the use of agar in solid media? A Robert Koch BWalter Hesse C Fannie Hesse D Fannie Koch ELouis Pasteur The structure that confers rigidity on the cell and protects it from osmotic lysis is known as the: A Cell Wall B Cytoplasmic membrane C Ribosome D Periplasmic membrane E Capsule

2.

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5. The outermost layer of an endospore is called the: A. Spore coat B. Exosporium C. Core wall D. Cortex E. Outer membrane 6. The thermal death time is: A. The time required for a tenfold reduction in the population density at a given temperature B. The time it takes to kill all cells at a given temperature C. The measure of the radiation output energy from a source D. The time it takes for a given dose of radiation to cause a tenfold reduction in the number of microorganisms

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The time it takes to kill 90% of all cells at a given temperature 7. Which of the following statements about phototrophic microorganisms is not true?
E.

A. Phototrophs are the foundation of the carbon cycle B. Anoxygenic phototrophs generate ATP via cyclic phosphorylation C. All phototrophs produce chlorophyll or bacteriochlorophyll to be photosynthetic D. Carotenoids primary role is to absorb light energy E. Oxygenic phototrophs use water (H2O) as an electron donor 8. If you have a microorganism that uses carbon dioxide as a carbon source and hydrogen as an energy source (electron donor) and grows optimally at 75C and pH 10, what terms can you apply to this organism? A hyperthermophile, chemolithotroph, heterotroph, acidophile B. thermophile, chemoorganotroph, autotroph, alkaliphile C. thermophile, chemolithotroph, autotroph, acidophile D. hyperthermophile, chemoorganotroph, heterotroph, alkaliphile E. thermophile, chemolithotroph, autotroph, alkaliphile 9. Anammox is the oxidation of ammonia with _______________ as the electron _______________. A. nitrite (NO2-); donor B. nitrite (NO2-); acceptor C. nitrate (NO3-); donor D. nitrate (NO3-); acceptor E. hydrogen (H2); donor
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Identification of the three domains of life, Bacteria, Archaea, and Eukarya, has come about primarily as a result of:

A. analyses of metabolic pathways. B. biochemical assays. C. DNA sequence-based phylogenetic analyses. D. advanced methods of morphological examination. E. cultivation of microorganisms. Section B. Short answer questions: (4 marks each)
1.

List Kochs postulates. 1. the suspected pathogen must be present in all cases of the diease and absent from the healthy animals 2. the suspected pathogen must be grown in pure culture 3. the cells from the pure culture of the suspected pathogen must cause disease in healthy animal. 4. the suspected pathogen must be reisolated and shown to be the same as the original

2.

List the steps required to conduct a Gram stain and describe the appearance of the two types of bacteria identified by this procedure. Fixation, crystal violet staining, iodine trapping, decolourisation, counterstaining with safranin Discuss how penicillins work with respect to their target and mode of action. Penicillin targets the transpeptidase enzyme binding to them which inhibits the formation of peptidoglycan, the penicillin bound transpeptidase will also stimulate the release of autolysin enzymes Name four properties of the 16S rRNA gene that make it suitable for measuring microbial diversity. Explain why nitrate (NO32-) is the preferred electron acceptor in anaerobic respiration.

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