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Definition of Terms

The document defines key terms used in microbiology and bacterial identification tests. It provides conceptual and operational definitions for analysis of variance, biochemical test, blood agar, citrate utilization test, culture, Escherichia coli, Gram stain, Gram-positive and Gram-negative bacteria, incubator, indole test, IMViC test, isolates, MacConkey agar, methyl red test, Staphylococcus aureus, and Voges Proskauer test. These definitions explain the purpose and procedures for various tests used to identify and differentiate bacterial species.

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1K views3 pages

Definition of Terms

The document defines key terms used in microbiology and bacterial identification tests. It provides conceptual and operational definitions for analysis of variance, biochemical test, blood agar, citrate utilization test, culture, Escherichia coli, Gram stain, Gram-positive and Gram-negative bacteria, incubator, indole test, IMViC test, isolates, MacConkey agar, methyl red test, Staphylococcus aureus, and Voges Proskauer test. These definitions explain the purpose and procedures for various tests used to identify and differentiate bacterial species.

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Irish Orleans
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We take content rights seriously. If you suspect this is your content, claim it here.
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Definition of Terms The following terms are defined conceptually and operationally in this study for the purpose

of better understanding and communication of ideas: Analysis of variance (ANOVA) a term refers to a series of statistical procedures for comparing differences among three or more groups, rather than testing each pair of means separately, to determine if differences are due to chance. It is accomplished by examining the differences within the groups as well. (www.medicaldictionary.thefreedictionary.com) Biochemical Test it is a test that help in Identification of several Bacterial isolates Blood Agar - a general purpose enriched medium often used to grow fastidious organisms and to differentiate bacteria based on their hemolytic properties. (American Society of Microbiology 2012) Citrate Utilization Test - This test detects the ability of an organism to utilize citrate as the sole source of carbon and energy. Bacteria are inoculated on a medium containing sodium citrate and a pH indicator bromothymol blue. The medium also contains inorganic ammonium salts, which is utilized as sole source of nitrogen. (www.microrao.com)

Culture - The growing of microorganisms, tissue cells, or other living matter in a specially prepared nutrient medium; Such a growth or colony, as of bacteria. (www.medical-dictionary.thefreedictionary.com) Escherichia coli - a term which pertain to microorganism that is a Gramnegative, rod-shaped bacterium that is commonly found in the lower intestine of warmblooded organisms (www.wikipedia.com) Gram stain - is the most extensively performed procedure in diagnostic microbiology. It is used to classify bacteria as either gram-positive or gram-negative based upon their ability to retain the crystal violet stain following decolorization. In addition, the Gram stain provides vita0l diagnostic information, aids in the selection of culture media. (www.healthline.com) Gram-positive bacteria a term that refers to those bacteria whose cell wall retains a stain know as Grams stain or resists decolorization with alcohol during culture and sensitivity testing. (Karch, 2011) Gram-negative bacteria a term that refers to those bacteria whose cell walls lose a stain or are decolorized by alcohol. (Karch, 2011) Incubator a term which pertain to a device for maintaining a bacteria culture at a particular temperature for a set length of time, in order to measure growth. (Websters Dictionary) Indole Test a procedure to detect some bacteria that can produce indole from amino acid tryptophan using the enzyme typtophanase.(www.microrao.com)

IMViC Test - are frequently employed for identification of this group of microbes which includes such organisms as Klebsiella, Enterobacter, and Escherichia coli. (MicroVision 1998) Isolates a term that refers to the population of microorganisms that has been obtained in pure culture from a field case or location. (www.medical-

dictionary.thefreedictionary.com)

MacConkey Agar - is a selective and differential medium used to isolate and differentiate members of the Enterobacteria often referred to as enterics. It is also used to isolate some Staphylococcus and Enterococcus (www.biology.missouristate.edu)

Methyl Red (MR) Test - This is to detect the ability of an organism to produce and maintain stable acid end products from glucose fermentation. Some bacteria produce large amounts of acids from glucose fermentation that they overcome the buffering action of the system. Methyl Red is a pH indicator, which remains red in color at a pH of 4.4 or less. (www.microrao.com)

Staphylococcus aureus - a term which pertain to microorganism stains grampositive and is non-moving small round shaped or non-motile cocci. It is found in grapelike staphylo- clusters. (http://www.news-medical.net) Voges Proskauer (VP) Test - detects butylene glycol producers (www.microrao.com)

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