Quiz 3 Protein Targeting
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JeevikaGoyalQuiz 3 Protein Targeting
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JeevikaGoyalQuestion 1 Figure 1: Plasma membrane labeled with RFP, protein tagged with GFP
1 / 1 point
Where in the cell are the GFP tagged proteins localized? Mitochondria Cytosol Golgi RER Question 2 1 / 1 point
Figure 2. Nuclei are stained with DAPI, secreted protein tagged with GFP
Where in the cell are the GFP-tagged proteins localized? Golgi Cytosol Nucleus RER Question 3 1 / 1 point A mutated PTS1 (Ser-Lys-Leu) targeting sequence on a catalase protein would result in.... Inability for the protein to be transported into the peroxisome Inability for the protein to be embedded in the peroxisomal membrane The protein to be transported into the peroxisome, but become non-functional No change in the proteins normal activity Targeting of the protein to the mitochondria Question 4 The mitochondiral matrix targetting motif is... Necessary, but not sufficient Sufficient, but not necessary Necessary and sufficient Neither necessary nor sufficient question set motifs Question 5 1 / 1 point A protein that has a serine-lysine-leucine motif at its C-terminus will likely end up in which organelle? Peroxisome Mitochondria 1 / 1 point
Nucleus ER Chloroplast Question 6 1 / 1 point A protein that has a motif rich in valines, threonines, and serines at its N-terminus will likely end up in which organelle? Peroxisome Mitochondria Nucleus ER Chloroplast Question 7 1 / 1 point
Researchers have the DNA for a new protein. They do some investigating and find that the DNA sequence is similar to a known mitochondrial protein in mouse. They want to determine if this new protein is also a localized to the mitochondria in humans. They decide to create a DNA construct which, when translated, will create a fusion protein with their new protein and GFP. They also purchase from a supplier a RFP (red fluorescence protein) with the mitochondrial matrix targetting motif at its N-terminus. They introduce these two proteins into cultured HeLa (human cancer cells). Based on their hypothesis, they expect to see red and green in the same areas of the cell. Based on their hypothesis, they expect to see red and green in different areas of the cell. question set mito exp Question 8 0 / 1 point
The images the researchers obtained from the experiment described in Q7 are shown here. What can they conclude?
Their protein is not targetted to the mitochondria. Their protein is targetted to the mitochondia, but there is not enough evidence to indicate where in the mitochondria it is localized. Their protein is targetted to the mitochondial matrix. Their protein is targetted to the inner membrane of the mitochondia. Their protein is targetted to the inter-membrane space of the mitochondia. Their protein is targetted to the outer membrane space of the mitochondia. Question 9 1 / 1 point
In the video presentation on mitochondrial targeting Dr. Lithgow described protein complexes in the mitochondrial membranes that "direct traffic" into the various parts of the mitochondria. Which of the following is not one of the complexes named. TIMbit SAM TIM22 TOM Question 10 Lithgow set 1 / 1 point
In the video presentation on protein import to the mitochondria Dr. Lithgow described protein complexes in the mitochondrial membranes that "direct traffic" to the various parts of the mitochondria. To reach the inner membrane a protein must go through which sequence. through the TOM complex, to tiny TIMs, to the TIM22 complex. through the TOM complex, to tiny TIMs to the SAM complex. through the TOM complex to the TIM23 complex, then MPP cleaves the target sequence. through the TOM complex, to the TIM23 complex, then get processed by IMP. question set location Question 11 0 / 1 point A researcher has added an amphipathic alpha-helix motif to the C-terminus of GFP, which is normally a cytoplasmic protein. Where would you hypothesize this protein might end up in the cell? Cytoplasm Mitochondria Nucleus ER Peroxisome
View Feedback Check which end of the polypeptide the signal sequence needs to be! question set Question 12 0 / 1 point A researcher adds the mitochondrial hydrophobic stop-transfer sequence to GFP. Where would you expect to see GFP when visualizing with fluorescence microscopy? In the cytosol In the mitochondrial matrix In the inner membrane of the mitochondria In the inter-membrane space of the mitochondria In the outer membrane of the mitochondria Question 13 palade question 0 / 1 point
From the video lecture by Dr. Nicchitta on the endoplasmic reticulum the experiments of Jamieson and Palade showed movement of radiolabelled proteins through the cell. In this experiment, called a pulsechase, they determined the path these proteins take to be: From the nucleus to the RER, to the Golgi to the secretory vesicles. From the RER to the peripheral Golgi, to the condensing vacuoles of the Golgi, to secretory vesicles. From the RER to the condensing vacuoles of the Golgi, to the peripheral Golgi, to the mitochondria. From the RER to the condensing vacuoles of the Golgi, to the peripheral Golgi, to secretory vesicles. Question 14 From the video lecture by Dr. Nicchitta, the experiments of Blobel and Sabatini showed that: secreted proteins were synthesized in the cytosol and transported to the ER. secreted proteins were susceptible to protease during synthesis the RER is so named because it is free of ribosomes. secreted proteins were synthesized at the RER and passed into the lumen of the ER. 1 / 1 point
10 (71.43 Attempt Score: / %) 14 Overall Grade (highest attempt): 10 (71.43 / %) 14
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