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Epa Probit Analysis Program

This document contains the results of an EPA probit analysis used to calculate lethal concentration (LC) and effective concentration (EC) values. It presents observed and predicted response proportions from toxicity tests on Daphnia at three concentrations. It also includes chi-square and parameter estimates, confidence limits for LC/EC values from 1-99%, and a plot of adjusted probits and predicted regression line.

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Luthfy Wulandari
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1K views

Epa Probit Analysis Program

This document contains the results of an EPA probit analysis used to calculate lethal concentration (LC) and effective concentration (EC) values. It presents observed and predicted response proportions from toxicity tests on Daphnia at three concentrations. It also includes chi-square and parameter estimates, confidence limits for LC/EC values from 1-99%, and a plot of adjusted probits and predicted regression line.

Uploaded by

Luthfy Wulandari
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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EPA PROBIT ANALYSIS PROGRAM

USED FOR CALCULATING LC/EC VALUES


Version 1.5



LC50_24_Dhapnia_Fe

Proportion
Observed Responding Predicted
Number Number Proportion Adjusted for Proportion
Conc. Exposed Resp. Responding Controls Responding

50.0000 30 9 0.3000 0.3000 0.2857
100.0000 30 17 0.5667 0.5667 0.6067
150.0000 30 24 0.8000 0.8000 0.7764


Chi - Square for Heterogeneity (calculated) = 0.328
Chi - Square for Heterogeneity
(tabular value at 0.05 level) = 3.841

Mu = 1.902617
Sigma = 0.359834

Parameter Estimate Std. Err. 95% Confidence Limits
---------------------------------------------------------------------
Intercept -0.287479 1.421857 ( -3.074318, 2.499361)
Slope 2.779056 0.724478 ( 1.359080, 4.199032)

Theoretical Spontaneous Response Rate = 0.0000


LC50_24_Dhapnia_Fe

Estimated LC/EC Values and Confidence Limits

Exposure 95% Confidence Limits
Point Conc. Lower Upper

LC/EC 1.00 11.629 1.341 23.786
LC/EC 5.00 20.451 4.217 34.878
LC/EC 10.00 27.635 7.740 42.918
LC/EC 15.00 33.860 11.631 49.498
LC/EC 50.00 79.913 58.176 101.122
LC/EC 85.00 188.603 137.609 436.855
LC/EC 90.00 231.090 159.418 653.517
LC/EC 95.00 312.254 196.871 1195.211
LC/EC 99.00 549.161 289.525 3746.143

LC50_24_Dhapnia_Fe

PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE

Probit
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- .o..
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5+ ...
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