Scribd
Upload
1K views

Dna Replication Worksheet

DNA replication involves unwinding the double helix by helicase and separating the strands. DNA polymerase then forms new complementary strands in the 5' to 3' direction by matching nucleotides to the template strands through complementary base pairing. This process is semi-conservative, meaning the double helix splits into two double helices, each with one original and one new strand.

Uploaded by

John Osborne
Copyright
© © All Rights Reserved
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
1K views

Dna Replication Worksheet

DNA replication involves unwinding the double helix by helicase and separating the strands. DNA polymerase then forms new complementary strands in the 5' to 3' direction by matching nucleotides to the template strands through complementary base pairing. This process is semi-conservative, meaning the double helix splits into two double helices, each with one original and one new strand.

Uploaded by

John Osborne
Copyright
© © All Rights Reserved
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
You are on page 1/ 1

DNA REPLICATION

Core syllabus
Explain DNA replication in terms of unwinding the double helix and separation of the strands by helicase
(enzyme), followed by the formation of the new complementary strands by DNA polymerase (enzyme).
Explain the significance of complementary base pairing in the conservation of the base sequence of DNA.
State that DNA replication is semi-conservative and use a diagram to illustrate what semi-conservative
replication of DNA means.
State that large portions as much as 90% - of DNA are not coding genes. This used to be called Junk DNA.
HL only
State that DNA replication occurs in the 5 -> 3 direction.
The 5 end of the free DNA nucleotide is added to the 3 end of the chain of nucleotides that is already
synthesised. Nucleotides can only be added to the 3 C of ribose sugar.
Explain the process of DNA replication in prokaryotes, including the role of enzymes (helicase, DNA
polymerase, RNA primase and DNA ligase), Okazaki fragments and deoxynucleoside triphosphates.
The explanation of Okazaki fragments in relation to the direction of DNA polymerase III action is
required. DNA polymerase III adds nucleotides in the 5 -> 3 direction. DNA polymerase I excises the
RNA primers and replaces them with DNA.
State that DNA replication is initiated at many points in eukaryotic chromosomes.

OUTCOMES Core & HL


1. Overall notes, taken according to the Cornell template
2. Diagram of the cell cycle, showing the phase of the cycle when DNA replication occurs. (Revision)
3. State the functions of the enzymes:
1. Helicase
2. DNA polymerase
4. Draw diagrams to explain what is meant by semi-conservative and justify saying that the replication of
DNA is semi-conservative.
5. Draw a simple diagram, which includes all 4 of the nitrogen bases present in DNA, which shows the idea
of complementary base pairing.
6. Watch the YouTube video: http://www.youtube.com/watch?v=teV62zrm2P0 (There are a whole mass of
useful YouTube videos you can watch. Dont get too carried away! Watch out for HL detail which SL
students dont need.)
HL should also watch: http://www.youtube.com/watch?v=yqESR7E4b_8 which helps understand the way
DNA is coiled around it protein skeleton.

HL only
7.
8.
9.

By means of a labelled diagram of a small piece of prokaryotic DNA which is undergoing replication, show
how the replication occurs in 5 to 3 direction, as well as showing the replication process. This diagram
needs to be labelled specifically to the HL needs (see above).
By means of a suitable image taken from the internet, describe how Hershey and Chase provided
experimental evidence that DNA must be the genetic material.
List the following enzymes and proteins involved in the replication of DNA, and state a specific function
for each: helicase, DNA gyrase, single strand binding protein, DNA primase, DNA polymerase I & III.

You might also like