Biochemistry

Basic Medical Science

Time: 40 mins

Author Email: [email protected]

Total Marks: 50 | Pass Marks: 30

BIOCHEMISTRY
Please use a dark pencil/pen to complete the test. Read the instructions at the
begining carefully. This is an Sample Test Paper for practice.
Please Note: There is a Negative Marking of (-1) for each incorrect response.
1. In fatty acid synthesis, the enzyme required for priming acetyl-ACP with
malonyl-ACP for chain extension
A 3-ketoacyl-ACP reductase

C Acetyl CoA:ACP transacylase

B 3-ketoacyl-ACP synthase

D Malonyl CoA:ACP transacylase

2. An enzyme of the non-oxidative phase of the pentose phosphate pathway:

A Ribulose 5-Phosphate
3-Epimerase

C Methylcrotonyl-CoA carboxylase

B 6-phosphogluconate
dehydrogenase

D Acetyl-CoA carboxylase alpha

3. Biotin deficiency is characterized by all of the following except:

A hypercholesterolemia

C scaly dermatitis

B glossitis

D dementia

4. Match the following Vitamins with their daily recommended dietary intake as
supplement:
A.
B.
C.
D.
E.

Thiamine
Cobalamin (Vit.B12)
Niacin
Pantothenic acid (B5)
Biotin

A.
B.
C.
D.
E.

5 mg
1.1-1.2 mg
14-16 mg
2.4 mg
30 g

5. Carotenoids without Vitamin A activity:

A -carotene

C zeaxanthin

B -cryptoxanthin

D lycopene

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Biochemistry
6. The enzyme that catalyzes reductive amination of -ketoglutarate in prokaryotes
and plants
A glutamate synthase

C glutamate dehydrogenase

B glutaminase

D glutamine synthetase

7. Arrange the following substrates of the glycolytic pathway in their correct order of
events/reactions: (1 5)
A.
B.
C.
D.
E.

1,3-Bisphosphoglycerate
Fructose 1,6-bisphosphate
Phosphoenolpyruvate
Glyceraldehyde 3-phosphate
Glucose 6-phosphate

8. Hyperkeratosis can occur due to

A Vemurafenib

C chronic exposure to Mercury

B chronic exposure to Arsenic

D all of the above

9. What is not true about holoenzymes?

A DNA polymerases are examples
of holoenzyme

C cofactors in holoenzymes are

mostly enzymes or proteins

B contains cofactors weakly bound

to enzymes called prosthetic
groups

D apoenzyme + coenzyme

10. Match the following enzyme with their inorganic cofactors:

A.

catalase

A. manganese

B.
C.
D.
E.

cytochrome
hexokinase
arginase
nitrate reductase

B.
C.
D.
E.

magnesium
molybdenum
ferrous or ferric
cupric

11. An example of oxidoreductase enzyme

A chymotrypsin

C lactate dehydrogenase

B fumarase

D aminoacyl-tRNA synthetase

12. The pyruvate dehydrogenase multienzyme complex require all of the following
cofactors except:
A thiamine pyrophosphate (TPP)

C favin adenine dinucleotide (FAD)

B cobalt

D magnesium

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Biochemistry
13. The enzyme that converts glutamine to glutamate and NH4+ in liver mitochondria
A glutamine synthetase

C glutamate dehydrogenase

B glutamate synthase

D glutaminase

14. Serine is synthesized from:

A glutamate

C -ketoglutarate

B 3-phosphoglycerate

D 3-phosphohydroxypyruvate

15. Aminoacid threonine is synthesized from

A 3-phosphoglycerate

C aspartate

B pyruvate

D glutamine

16. Which of the following substances is/are responsible for brain edema due to
astrocyte swelling in hepatic encephalopathy?
A glutamine

C glutamate

B ammonia

D all of the above

17. Match the following enzymes with their correct classification subgroup:
A.
B.
C.
D.
E.

phospholipase C
lactate dehydrogenase
phosphoglucomutase
fumarase
UDP-glucose pyrophosphorylase

A.
B.
C.
D.
E.

Hydrolase
Transferases
Oxidoreductase
Isomerase
Lyases

18. Formation of glutamine is catalyzed by

A glutamine synthetase

C glutamate synthase

B glutaminase

D glutamate dehydrogenase

19. Which of the following is an endogenous NMDA receptor antagonist?

A dextromethorphan

C kainic acid

B kynurenic acid

D glutamic acid

20. Which of the following enzyme is a lyase?

A glutamine synthetase

C aspartate transcarbamylase

B histidine decarboxylase

D Uracil-DNA glycosylase

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Biochemistry
21. Which of the following is not true about Vitamin E?
A regulation of prostaglandin
synthesis

C protects integrity of normal cell

membranes

B large doses may induce Vitamin

A deficiency

D large doses may induce

hemolysis of RBC

22. Which of the following is a xanthophyll carotenoids?

A -carotene

C lutein

B lycopene

D -cryptoxanthin

23. Which vitamin is necessary for metabolic oxidation of tyrosine?

A Vitamin C

C Pyridoxine

B Vitamin E

D Biotin

24. An essential amino acid

A proline

C tryptophan

B alanine

D tyrosine

25. Which of the following is a cofactor of Methylmalonyl Coenzyme A mutase (MUT)?

A methylcobalamin

C cyanocobalamin

B adenosylcobalamin

D hydroxocobalamin

26. Vitamin that is essential for formation of collagen

A Pyridoxine

C Vitamin E

B Vitamin D

D Vitamin C

27. Hormones that act through Ca2+ and phosphatidylinositol bisphosphate (PIP)
system
A oxytocin

C glucagon

B atrial natriuretic peptide (ANP)

D insulin

28. Enzyme that catalyzes the synthesis of fumarate from succinate and FAD
A succinyl dehydrogenase

C succinyl CoA synthetase

B -ketoglutarate dehydrogenase

D succinate dehydrogenase

29. Succinyl CoA is a[A]_____________ compound.

G for hydrolysis of ATP is [B]__________________.

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Biochemistry
30. Which of the following substrate inhibits carnitine acytransferase I?
A acetyl CoA

C palmitoyl CoA

B malonyl CoA

D succinyl CoA

31. Pick the incorrect statements about the tricarboxylic acid cycle (TCA cycle)
A involves oxidation of acetyl CoA

C require enzyme succinate

dehydrogenase which is a
mitochondrial matrix enzyme

B amphibolic cycle which provides

carbon skeleton for
gluconeogenesis, fatty acid
synthesis

D pathway for reduction and

reoxidation of coenzymes

32. Formation of methylmalonyl CoA from propionyl CoA require which vitamin as a
cofactor?
A Thiamine

C Vitamin B12

B Pyridoxine

D Vitamin B7

33. Schardinger enzyme is found in

A cheese

C boiled milk

B yoghurt

D raw milk

34. Which vitamin is required as a precursor in the biosynthesis of coenzyme A (CoA)?

A Panthothetic acid

C Pyridoxine

B Folic acid

D Biotin

35. Constituents of enzyme xanthine oxidase:

A riboflavin

C molybdenum

B iron atoms

D all of the above

36. Match the following cofactors with their sources:

A.
B.
C.
D.

Nicotinamide ring of NAD+

Flavin adenine dinucleotide FAD
Coenzyme A (CoA)
-ketoacid dehydrogenases

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A.
B.
C.
D.

Riboflavin
Niacin
Thiamine
Pantothenic acid

Biochemistry
37. Regarding -subunits of voltage gated sodium channels (Nav) true is:
A four repeat domains with six
membrane-spanning segments

C pore consisting selectivity filter

made of positively charges amino
acids

B six repeat domains with four

membrane-spanning segments

D -subunit Nav1.3 expressed in

dorsal root ganglia is associated
with pain

38. What is not true about pyruvate kinase?

C cofactor of pyruvate kinase is K+

A catalyzes the last reaction in

glycolysis

D is the key enzyme of glycolysis in

muscles

B is more active in fasting state

39. Which of the following enzymes require lipoic acid for its activity?
A pyruvate dehydrogenase

C -ketoacid dehydrogenase

B indole 2,3, dioxygenase

D superoxide dismutase

40. Which of the following enzyme is an oxidase / oxidoreductase?

A glutamate decarboxylase

C 2-furoyl-CoA dehydrogenase
2-furoyl-CoA dehydrogenase

B tyrosinase

D uridine monophosphate
synthetase

41. The gene that codes for enzyme Uridine monophosphate synthetase is located on
the
A short arm of chromosome 3 (3p13)
B short arm of chromosome 11 (3
p11)

C long arm of chromosome 11

(3q11)
D long arm of chromosome 3 (3q13)

42. Conversion of -carotene into Vitamin A occurs chiefly in the

A brain

C intestine

B kidney

D liver

43. Pellagra is due to deficiency of

A Niacin

C Thiamine

B Riboflavin

D Biotin

44. Wild type strain of bacteria that do not require any growth factors:
A Lactobacillus

C Haemophilus influenzae

B E. coli

D Neisseria meningitidis

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Biochemistry
45. Synthesis of Porphyrin ring requires all of the following except:
A acetyl CoA

C succinyl CoA

B glycine

D -Aminolevulinic acid

46. Amnesic shellfish poisoning is due to neurotoxin

A -N-methylamino-l-alanine

C domoic acid

B okadaic acid

D conotoxin

47. Which of the following is involved in growth and differentiation?

A Retinoic acid

C Retinol

B Retinal

D Retinene

48. What is not true regarding congenital erythropoietic porphyria?

A urine of patients appear red

C deficiency of cosynthase

B increased synthesis of
-aminolevulinate

D formation of large amount of

uroporphyrinogen I

49. The main storage form of vitamin K in animals:

A Vitamin K2

C phylloquinone

B Vitamin K1

D phytomenadione

50. Avidin binds to which vitamin and induces its deficiency?

A Vitamin B6

C Vitamin B12

B Vitamin B1

D Vitamin B7

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