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Agentes CDC

The document summarizes the four biosafety levels (BSLs) used for handling biohazardous materials. Each BSL consists of specific combinations of laboratory practices, safety equipment, and facilities appropriate for the infectious agents and laboratory activities. BSL-1 is for agents not known to cause disease in healthy adults. BSL-2 requires more containment and includes agents that pose moderate hazards. BSL-3 is for indigenous or exotic agents that may be transmitted through aerosols and cause serious disease. BSL-4 is the highest level, for dangerous exotic agents that pose high risks of life-threatening disease transmission via aerosols and have no available treatments. Examples of agents for each BSL are provided.

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27 views

Agentes CDC

The document summarizes the four biosafety levels (BSLs) used for handling biohazardous materials. Each BSL consists of specific combinations of laboratory practices, safety equipment, and facilities appropriate for the infectious agents and laboratory activities. BSL-1 is for agents not known to cause disease in healthy adults. BSL-2 requires more containment and includes agents that pose moderate hazards. BSL-3 is for indigenous or exotic agents that may be transmitted through aerosols and cause serious disease. BSL-4 is the highest level, for dangerous exotic agents that pose high risks of life-threatening disease transmission via aerosols and have no available treatments. Examples of agents for each BSL are provided.

Uploaded by

DanielRamosRamos
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Section1BiologicalSafety

Chapter8
BiosafetyLevels

BIOSAFETYLEVELSFourbiosafetylevels(BSLs)aresummarizedinthetablebelowforproper
handlingofbiohazardousmaterials.BSLsconsistofcombinationsoflaboratorypracticesand
techniques,safetyequipment,andlaboratoryfacilities.Eachcombinationisspecifically
appropriatefortheoperationsperformedthedocumentedorsuspectedroutesoftransmission
oftheinfectiousagents,andforthelaboratoryfunctiontoractivity.

BSL Agents

Practices

SafetyEquipment Facilities
(PrimaryBarriers) (SecondaryBarriers)

Notknowntoconsistently Standard
causediseasesin
microbiological
immunocompetentadult practices
humans

Nonerequired

Openbenchtop,sinkrequired

Associatedwithhuman
disease.Hazard:
percutaneousinjury,
mucousmembrane
exposure,ingestion

BSL1practicesplus:
limitedaccess
biohazardwarning
signs
sharpsprecautions
biosafetymanual
definingwaste
decontaminationor
medicalsurveillance
policies

Primarybarriers:ClassIor
IIbiosafetycabinetsor
otherphysical
containmentdevicesused
forallmanipulationsof
agentsthatcausesplashes
oraerosolsofinfectious
materials;PPE:laboratory
coats,gloves,face
protectionasneeded

BSL1plus:
nonfabricchairsandother
furnitureeasilycleanable
autoclaveavailable
eyewashreadilyavailable

Indigenousorexoticagents
withpotentialforaerosol
transmission;diseasemay
haveseriousorlethal
consequences

BSL2practicesplus:
controlledaccess
decontaminationof
allwastes
decontaminationof
labclothingbefore
laundering
baselineserum

Primarybarriers:ClassIor
IIbiosafetycabinetsor
otherphysical
containmentdevicesused
forallmanipulationsof
agents;PPE:laboratory
coats,gloves,respiratory
protectionasneeded

BSL2plus:
physicalseparationfromaccess
corridors
handsfreehandwashingsink
selfclosingdoubledooraccess
exhaustairnotrecirculated
negativeairflowintolaboratory
eyewashreadilyavailableinlab

Dangerous/exoticagents
whichposehighriskoflife
threateningdisease,
aerosoltransmittedlab
infections;orrelated
agentswithunknownrisk
oftransmission

BSL3practicesplus:
clothingchange
beforeentering
showeronexit
allmaterial
decontaminatedon
exitfromfacility

Primarybarriers:All
BSL3plus:
proceduresconductedin separatebuildingorisolatedzone
ClassIIIbiosafetycabinets dedicatedsupply/exhaust,vacuum
anddeconsystem
orClassIorIIbiosafety
cabinetsincombination
withfullbody,airsupplied
positivepressuresuit

SummarizedfromBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thEdition,2007.

http://www.cdc.gov/od/ohs/biosfty/bmbl5/bmbl5toc.htm

ClassificationofAgentsAccordingtoRisk
Biologicalagentsareassignedtobiosafetylevels(BSL)basedontherisktheyposetohuman
healthandtheenvironment.Suchfactorsasseverityofdiseasecausedbytheagentroutesof
exposure,andvirulenceareusedwhendeterminingthemostappropriateBSL.Thepartiallist
belowisprovidedtoassistlaboratoriesinmakingpreliminarydecisionsontheappropriate

Section1BiologicalSafety
Chapter8
BiosafetyLevels

biosafetylevelforparticularagents.Ultimately,theOccupationalandEnvironmentalSafety
Office(OESO)willmakethefinalBSLassignment.Ifaparticularagentisnotlistedbelow,orif
furtherassistanceisneededininterpretingBSLrequirements,contacttheOESOBiological
SafetyDivisionat6848822.

BiosafetyLevel1(BSL1)
BSL1issuitableforworkinvolvingwellcharacterizedagentsnotknowntoconsistentlycause
diseaseinimmunocompetentadulthumans,andpresentminimalpotentialhazardto
laboratorypersonnelandtheenvironment.Allbacterial,parasitic,fungal,viral,rickettsial,and
chlamydialagentswhichhavebeenassessedforriskbutdonotbelongtoahigherriskgroup
canbesafelyhandledatBSL1.Beawarethatmanyagentsnotordinarilyassociatedwith
diseaseareopportunisticpathogensandmaycauseinfectionintheyoung,theagedand
immunocompromisedindividuals.ExamplesofBSL1agentsinclude:Bacillussubtilis,Eschericia
coliK12,Naegleriagruberi,etc.

BiosafetyLevel2
ViralAgents:
Adenovirus
HTLVtypesIandII
CreutzfeldJacobagent
HumanBlood&BloodProducts
Cytomegalovirus
Kuru
Easternequineencephalitis
Monkeypoxvirus
EpsteinBarrvirus
SIV
HepatitisA,B,C,D,E
Spongiformencephalopathies
Herpessimplexviruses
Vacciniavirus
HIV
VSV(labadaptedstrains)

Bacterial/RickettsialAgents:
Campylocacterfetus,coli,jejuni
Shigellaboydii,dysenteriae,
Chlamydiapsittaci,trachomatis
flexneri,sonnei
Clostridiumbotulinum,tetani
Treponemapallidum
Corynebacteriumdiphtheriae
Vibriocholera
Legionellaspp
(includingElTor)
Neisseriagonorrhoeae
Vibrioparahemolyticus
Neisseriameningitidis
Vibriovulnificus
Pseudomonaspseudomallei
Yersiniapestis
Salmonellaspp

FungalAgents:
Blastomycesdermatitidis
Fonsecaeapedrosoi
Cryptococcusneoformans
Sporothrixschenkii
Microsporumspp
Trichophytonspp
Exophialadermatitidis(wangiella)

Section1BiologicalSafety
Chapter8
BiosafetyLevels

ParasiticAgents:
Entomeoebahistolytia
Strongyloidesspp
Crytosporidiumspp
Taniasolium
Giardiaspp
Toxoplasmaspp
Naegleriafowleri
Trypanosomaspp
Plasmodiumspp

BiosafetyLevel3
ViralAgents:
Valley
VSVexoticstrains(Piry)
RiftValleyFever(Zinga)
Yellowfever(wildtype)

Bacterial/RickettsialAgents:
Mycobacteriumbovis
Bacillusanthracis
Rickettsiarickettsii
Francisellatularensis
Yerseniapestis(resistantstrains)
Mycobacteriumtuberculosis

FungalAgents:
Coccidioidesimmitis
Histoplasmacapsulatum

BiosafetyLevel4
ViralAgents:
HemorrhagicFevers:
Herpessimiae(Bvirus)
(CongoCrimean,Junin,Machupo)
Lassa
Ebola
Marburg

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