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Microscope Calculations

1. This document provides examples and questions about microscope calculations and measurements. It includes converting between millimeters and microns, calculating total microscope magnifications using different objective and ocular lenses, determining field of view sizes under low and high power, and calculating cell sizes visible under microscopic views. 2. Questions involve calculating microscope magnifications and field of views using the formulas that relate high power magnification, low power magnification, and their respective field of views. Examples demonstrate measuring cell lengths visible under different microscope lenses and magnifications. 3. The document serves as a guide for understanding basic microscope specifications and calculations to determine sizes and magnifications visible when viewing samples under various microscope configurations and lenses.

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4K views

Microscope Calculations

1. This document provides examples and questions about microscope calculations and measurements. It includes converting between millimeters and microns, calculating total microscope magnifications using different objective and ocular lenses, determining field of view sizes under low and high power, and calculating cell sizes visible under microscopic views. 2. Questions involve calculating microscope magnifications and field of views using the formulas that relate high power magnification, low power magnification, and their respective field of views. Examples demonstrate measuring cell lengths visible under different microscope lenses and magnifications. 3. The document serves as a guide for understanding basic microscope specifications and calculations to determine sizes and magnifications visible when viewing samples under various microscope configurations and lenses.

Uploaded by

api-292439032
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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MICROSCOPE CALCULATIONS

1.

Change the following measurements to microns (m):


a.
b.
c.

2.

d.
e.
f.

0.03 mm
0.007 mm
1.04 mm

Change the following measurements to millimetres (mm)


a.
b.
c.

3.

5.0 mm
2.45 mm
0.20 mm

1765 m
540.1 m
1.1 m

d.
e.
f.

301.04 m
7002 m
49.7 m

Give the total magnification for a microscope if the following lenses are used:
OCULAR

OBJECTIVE

x5
x10
x15
x20

x10
x4
x4
x20

MAGNIFICATION

4.

Calculate the H.P. field of view of a microscope (ocular x5, objective x40) when
the L. P. magnification (ocular x5, objective x10) gives a field of view of 1.2 mm.

5.

A microscope has an ocular ( x15 ) and two objectives ( x10 and x40 ).

6.

a.

What is the H.P. magnification?

b.

What is the L.P. magnification?

c.

If the L.P. field of view is 3000 m, calculate the H.P field of view.

d.

Calculate the size of a microbe seen under the microscope if 12 of them fit
across the L.P. field of view of this microscope.

What is the L.P. field of view of each of these three different microscopes in
a. mm?
b. m?
A
B
C

millimetre rule

mm
m
7.

mm
m

mm
m

If the L.P. magnification in (A) above is x100, calculate the H.P. field of view for
microscope (A) when using a x5 ocular and x40 objective lens

8.

Remember 1 mm = 1000 m (microns or micrometers)


HP magnification x HP fov = LP magnification x LP fov
fov = field of view

Example 1:

What is the length of one cell in


a. mm
50 m

b. m

Example 2:
Using a 10x ocular and a 10x objective lens, an algal filament appears as shown:
Total magnification =
20 m

If a new objective (x40) was used, draw


what you would expect to see.
New magnification
Calculate the new field diameter:

Example 3:
If a high power magnification of 500x produces a 90 m field of view, what would be
the low power field of view when using the low power magnification of 100x?

Example 4:
Under low power magnification ( 90x ) we see the following:

1000 m
Under high power magnification of 450x we
see the image on the left. What is the
approximate diameter of each cell?

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