Scribd
Upload
98 views20 pages

Genes Reporteros

This document discusses reporter genes that are commonly used in biotechnology and genetic engineering. It provides examples of typical reporter genes such as lacZ, CAT, Gus, GFP, and Luciferase. For each reporter gene, it lists the gene product and describes common assays used to detect expression. It also includes diagrams of luciferase reactions and descriptions of experiments using reporter genes to detect gene expression.

Uploaded by

María Marco Clavero
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
98 views20 pages

Genes Reporteros

This document discusses reporter genes that are commonly used in biotechnology and genetic engineering. It provides examples of typical reporter genes such as lacZ, CAT, Gus, GFP, and Luciferase. For each reporter gene, it lists the gene product and describes common assays used to detect expression. It also includes diagrams of luciferase reactions and descriptions of experiments using reporter genes to detect gene expression.

Uploaded by

María Marco Clavero
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 20

Grado en Biotecnologa

Ingeniera gentica
Marcadores gnicos
(Genes reporteros)
PROFESORA: MARIA F. FILLAT

CAT

Typical reporter genes are:


Gene

Product

Assay

lacZ

beta-galactosidase

indicator plates, colorimetric


enzyme assay of cell extracts,
selection for Lac+

CAT

chloramphenicol enzyme assay or ELISA for gene


acetyltransferase product, selection for Cm resistance

Gus

beta-glucuronidase

GFP

green fluorescent protein

Luciferase

light

indicator plates, colorimetric


enzyme assay of cell extracts - used a lot in plants

fluorescence of colonies,
fluorescence of cells or
subcellular compartments,
imaging live cells
light measurement

luciferin + ATP luciferyl adenylate + PPi


luciferyl adenylate + O2 oxyluciferin + AMP + light

Filo Cnidaria
Gnero Renilla
Classe Anthozoa
Subclasse Anthomedusae

Expresin de b-glucuronidasa

pACE-green

Sites of Noggin Gene Expression


Mouse embryo containing a beta-galactosidase reporter gene replacing the
normal noggin gene. Beta galactosidase is revealed by the blue stain and
indicates sites of expression of the noggin gene in the brain and in the
skeleton. See Brunet, L. J., McMahon, J. A., McMahon, A. P., Harland, R. M.
(1998) Noggin, cartilage morphogenesis, and joint formation in the
mammalian skeleton. Science, 280, 1455-1457.

http://ls.berkeley.edu/node/329

Mutant and transgenic lines of P. berghei


Generation of a number of mutant, transgenic and knock-out parasite lines
which are used in our studies. For example parasite lines that express
transgenes such as Green Fluorescence Protein (GFP) and Luciferase.
These paraites are used to investigate specific interactions of the parasite
with cells of their hosts.

http://www.systembio.com/lentiviral-technology/transcriptionreporter-vectors/overview

You might also like