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Step and Flow Chart For Analysis

The document provides a step-by-step flow chart for sampling and analyzing ammonia gas concentrations. It involves: 1) assembling a gas sampling apparatus and collecting samples over 1 hour at 1 L/min, 2) preparing reagents and standardizing solutions, and 3) transferring sample solutions to flasks, adding reagents, and measuring absorbance to determine ammonia concentration compared to a calibration curve.

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Gopal Mallick
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112 views

Step and Flow Chart For Analysis

The document provides a step-by-step flow chart for sampling and analyzing ammonia gas concentrations. It involves: 1) assembling a gas sampling apparatus and collecting samples over 1 hour at 1 L/min, 2) preparing reagents and standardizing solutions, and 3) transferring sample solutions to flasks, adding reagents, and measuring absorbance to determine ammonia concentration compared to a calibration curve.

Uploaded by

Gopal Mallick
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Step wise flow chart

Adjust the Flow at 1L/min of the rotameter and the manifolds of the gas sampling
attachment of HVD/RDS
Place 10ml of absorbing media in each midget impinger for samples and field blanks
Assemble (in order) prefilter & holder, flowmeter, impinger and pump Sample at the
rate of 1L/min for 1hour duration
Record the sampling time, average folw rate and final volume of the solution After the
sample collection, transfer the solution in the impinger to polyethylene bottle and
recap it tightly for transport to laboratory for analysis
Prepare the absorbing media, various reagents and working solutions as per the method
described in protocol
Standardize the sodium thiosulphate solution by titration it against potassiu iodate and
Sodium hypochlorite by titration it standardized sodium thiosulphate solution
Take 25ml measurering flasks and rinse with distilled water. Transfer the contents from
polyethylene bottles 25ml measuring flasks (Maintian all the solutions at 25C)
Add 2ml of buffer (to maintain pH )
Add 5ml of working phenol solution, mix fill to about 22ml with distilled water and store
in the dark for 30mins to develop colour. Measure the absorbance of the solution at
630nm using Spectrophotometer.
Pipette 0.5, 1.0, and 1.5ml of working standard solution (working ammonia solution ) in
25ml measuring flasks
Fill to 10ml mark with absorning solution (0.1M H2SO4). Add the reagents as to each flask
as in the procedure for analysis
Read the absorbance of each standard against the reagent blank.
Plot the calibration curve
Calculate the concentration of NH3 in g/m3

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