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LS-CBB-5530 Cellular and Molecular Biology Gogol Exam 2 Nov. 4, 2014

This document is an exam for a cellular and molecular biology course testing knowledge of transcription and translation in E. coli. It consists of 5 pages with 1-3 multiple choice questions per page and instructs students to choose and answer one question per page for a total of 5 questions. It provides instructions on circling the chosen question number, writing answers below the questions, using the back of pages if needed, making drawings to illustrate answers, and allocating time to answer all questions before spending extra time on bonuses. It allocates 75 minutes for the exam and offers up to 3 bonus points for suggested improvements to the course content or presentation.

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52 views

LS-CBB-5530 Cellular and Molecular Biology Gogol Exam 2 Nov. 4, 2014

This document is an exam for a cellular and molecular biology course testing knowledge of transcription and translation in E. coli. It consists of 5 pages with 1-3 multiple choice questions per page and instructs students to choose and answer one question per page for a total of 5 questions. It provides instructions on circling the chosen question number, writing answers below the questions, using the back of pages if needed, making drawings to illustrate answers, and allocating time to answer all questions before spending extra time on bonuses. It allocates 75 minutes for the exam and offers up to 3 bonus points for suggested improvements to the course content or presentation.

Uploaded by

goutham278
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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LS-CBB-5530

Gogol
Exam 2
2014

Cellular and Molecular Biology


Nov. 4,

NAME:
___________________________________________________________________
____

There are five pages (AA-EE) to this exam, each with two or three
questions per page. For each page of the exam, choose ONE and
only one question to answer. Indicate which question you are
answering by circling its number.
Each question will be scored out of 20 points, so be sure to answer
five questions.
Answer all parts of the questions you choose. Read the question
carefully to make sure you are addressing it, not some other
question you may prefer to answer. Irrelevant information will not
score any bonus points.
You should be able to write your answer on the front of the page,
below the questions; if you absolutely need more space, you may
use the back of the page.
In some cases, you may find it useful to make a small clear drawing to
illustrate and clarify your answer. If you do so, please label the
components of the drawing.
Do not mix up transcription and translation they are both subjects of
this test.
You will have 75 minutes (assuming you arrive on time) for this exam,
so do not spend too long on any single question, at least until you
have answered all of your chosen ones.
If you have time, you may earn up to three bonus points on page FF by
suggesting a change in the content or presentation of this part of
the course (transcription and translation) that would improve it in
the future. (Its only a maximum of 3 points, so dont sacrifice

answering a 20-point question if you cant spare the time you will
get a chance to comment in the course evaluations at the end of
the semester.)

27

A1. Describe all the elements of an E. coli promoter that may


contribute to regulation of its transcription, and the interactions
the elements have with RNAP.
OR:
A2. What is the role of ppGpp in E. coli? When and how is it made, and
for what purpose? How does it affect transcription?

28

B1. Describe the experimental methodology (other than crystal


structure) that measured the length of the ssDNA in an open
promoter complex.
OR:
B2. Describe the process by which E. coli enters stationary phase,
including any factors used, and their purpose.

29

C1. The trp operon has two methods of regulation in E. coli. Describe
the mechanisms involved in both methods.
OR:
C2. Describe the role of DNA looping in regulation of the ara operon in
E. coli, including all the DNA sites and any proteins that may be
involved.
OR:
C3. What is CAP protein in E. coli, how is it activated, which genes are
affected, and how does it interact with RNAP?

30

D1. Describe two mechanisms of regulating eukaryotic translation.


Why is regulation of gene expression at the translational level so
much more rare in prokaryotes?
OR:
D2. Explain the differences in the mechanisms of ribosome binding to
mRNA in prokaryotes vs. eukaryotes. Make sure to state any
factors and ribosomal sites that are involved.
OR:
D3. Explain how poliovirus takes over translation in human cells, i.e.,
how it slows or stops host translation and promotes transcription
of viral mRNA.

31

E1. Eukaryotes hydrolyze two GTPs for each amino acid added to a
growing polypeptide. How and why are these two GTPs
hydrolyzed? (Name the factors and identify the specific steps they
promote.)
OR:
E2. What is the evidence for a rotation of the two ribosomal subunits
with respect to each other, and what is the experimental evidence
that this rotation plays a necessary role in translation?
OR:
E3. List the factors and explain the steps in translation termination and
ribosome recycling in bacteria.

32

Bonus (up to three points): Suggest a change in the content or


presentation of the transcription & translation part of this course
(the past 5 weeks) that would make it a better learning experience
for you a practical change that can actually be put into action,
should the instructor agree.)

33

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