The Executive Summary of the study:

1. The analysis of OSI of vegetable oils:

High Oryzanol Rice Bran Oil(HO RBO) has relatively high Oxidative Stability Index(OSI) .
By adding HO RBO into the oils with low OSI,OSI of blended oil is improved clearly.
OSI test is commonly used by vegetable oil industry to compare the oxidative stability of oils
The OSI is defined as the point of maximum change of the rate of oil oxidation. The method
used for OSI tests measures the induction period which is the length of time before the
start of rapid acceleration of oil oxidation, and it indicates the stability or resistance of the
fat or oil to oxidation.""The lower number the less inherent stability of the oil."

2. The analysis of PV of vegetable oils:

Peroxide Value (PV) is used to determine the primary products of lipid oxidation reactions.
PV of less than 3 meq/Kg indicates good oil quality.

3. The Fatty Acid Composition :

Typically, oils with high saturated and mono-unsaturated fatty acid content are very stable.
However, there is sufficient clinical evidence to support that saturated fat, trans fat, and
cholesterol intake play some role in increasing cardiovascular heart disease risk.
The American Heart Association recommends a healthy dietary pattern and lifestyle to
combat heart disease, limiting trans fat consumption to less than 1% (or approximately
2 g on a 2,000-calorie diet) and saturated fat to less than 7% of total daily calories.
It is very important that total calorie content of the foods is emphasized. The American
Heart Association supports the recommendations of the Institute of Medicine and
the National Cholesterol Education Program for total fat, which is specified at a range
of 25% to 35% of energy.

4.Conclusion :
Blending is an effective method to formulate oils with desirable fatty acid composition
(no trans and low saturated fatty acid content oils). HO RBO is an excellent product to
formulate oil blends with high oxidative stability and healthy fatty acid composition.
Our tests clearly demonstrate that blending HO RBO with cotton seed, canola, Nusun
and HO safflower improves the oxidative stability of the blends. Specifically cotton/HO
RBO blend has significantly lower saturated fatty acid content and high oxidative
stability than pure cotton seed oil.

Oilseeds International, Ltd.

8 Jackson Street,San Francisco, CA 9411
Phone: 415-956-7251
Fax: 415-394-9023
Email: [email protected]

Executive Summary
 8 Jackson Street,San Francisco, CA 9411
Phone: 415-956-7251
Fax: 415-394-9023
Email: [email protected]

Executive Summary
xidative Stability Index(OSI) .
d oil is improved clearly.
mpare the oxidative stability of oils.
e rate of oil oxidation. The method
s the length of time before the
the stability or resistance of the
t stability of the oil."

f lipid oxidation reactions.

cid content are very stable.

urated fat, trans fat, and
eart disease risk.
pattern and lifestyle to
n 1% (or approximately
7% of total daily calories.
s emphasized. The American
stitute of Medicine and
hich is specified at a range

fatty acid composition

an excellent product to
hy fatty acid composition.
cotton seed, canola, Nusun
Specifically cotton/HO
nd high oxidative

Oilseeds International, Ltd.

Jackson Street,San Francisco, CA 94111
Phone: 415-956-7251
Fax: 415-394-9023
Email: [email protected]

Executive Summary
Jackson Street,San Francisco, CA 94111
Phone: 415-956-7251
Fax: 415-394-9023
Email: [email protected]

Executive Summary
Test Result Summary by OSU

1.PV
Product Control Blend(25%) Blend(50%)
Cotton 1.11 1.59 1.50
Nusun 0.70 1.68 1.91
HO Saf 0.65 1.29 1.33
Canola 0.27 0.85 0.80
HO Sun 0.25 0.91 0.97
HO canola 0.24 0.93 1.17

RBO 1.18
HO RBO 1.37

2.OSI Test (*1)

Product Control Blend(25%) Blend(50%)
Cotton 6.79 8.54 9.68
Nusun 8.93 9.04 10.82
HO Saf 11.68 12.81 12.37
Canola (*2) 10.98 11.20 11.84
HO Sun 19.33 16.26 15.14
HO canola 21.32 17.59 14.69

RBO 11.98
HO RBO 12.90

*1:All of the test were done in Oklahoma State University in triplicate using a 743 Rancimat,
(the supplier is Metrohm Ion Analysis, Metrohm Ltd. CH-9101 Herisau Switzerland)
at an air flow rate of 20 L/h and temperature is 110 degree C

* 2 :Typical Canola Oil's OSI is approximately 7 or 8 . However, due to the lower linoleic
and linolenic supplied by JRI, the OSI of Control of canola oil is 11 , much higher than
regular canola oil.

Test Result
 Blend(75%)
1.28
1.39
1.17
1.37
1.06
0.93

Blend(75%)
11.05
11.33
12.71
12.02
13.81
13.77

ing a 743 Rancimat,

Switzerland)

e lower linoleic
h higher than

Test Result
1.COTTON OIL/RICE BRAN OIL BLEND

1.PV Test

Control High Oryzanol Rice Bran Oil Blend

Product Cotton Oil 100% Blend(25%) Blend(50%)

Cotton Oil 1.11 1.59 1.50

2.OSI Test

Control High Oryzanol Rice Bran Oil Blend

Product Cotton Oil 100% Blend(25%) Blend(50%)

Cotton Oil 6.79 8.54 9.68

Improvement VS. Controll + 1.75 + 2.89

3.Fatty Acid Compositon

Control High Oryzanol Rice Bran Oil Blend

Cotton Oil 100% Blend(25%) Blend(50%)

Palmitic Acid(C16-0) 22.4% 20.6% 18.7%

Stearic Acid(C18-0) 2.4% 2.3% 2.2%

Oleic Acid(C18-1) 19.6% 25.6% 30.8%

Linoleic Acid(C18-2) 52.3% 47.8% 44.0%

Linolenic Acid(C18-3) 0.2% 0.4% 0.7%

Other 3.2% 3.2% 3.6%

100% 100% 100%

Total (C:16:0/C18:0) 24.9% 22.9% 20.9%

Saturated Fat Increase(C16:0/C18:0) -2.0% -3.9%

COTTON OIL
ce Bran Oil Blend

Blend(75%)

1.28

ce Bran Oil Blend

Blend(75%)
11.05

+ 4.26

ce Bran Oil Blend

Blend(75%)

16.8%

2.1%

36.2%

40.2%

1.0%

3.8%

100%

18.9%

-6.0%

COTTON OIL
2.CANOLA OIL/RICE BRAN OIL BLEND

1.PV Test

Control High Oryzanol Rice Bran Oil Blend

Product Canola 100% Blend(25%) Blend(50%)

CANOLA OIL 0.27 0.85 0.80

2.OSI Test

Control High Oryzanol Rice Bran Oil Blend

Product Canola 100% Blend(25%) Blend(50%)

CANOLA OIL 10.98 11.20 11.84

Improvement VS. Controll + 0.22 + 0.86

3.Fatty Acid Compositon

Control High Oryzanol Rice Bran Oil Blend

Canola 100% Blend(25%) Blend(50%)

Palmitic Acid(C16-0) 4.0% 6.3% 9.4%

Stearic Acid(C18-0) 1.9% 1.9% 2.0%

Oleic Acid(C18-1) 63.5% 59.0% 52.7%

Linoleic Acid(C18-2) 18.9% 22.5% 27.4%

Linolenic Acid(C18-3) 7.6% 6.2% 4.5%

Other 4.1% 4.1% 4.1%

100% 100% 100%

Total (C:16:0/C18:0) 5.9% 8.2% 11.3%

Saturated Fat Increase(C16:0/C18:0) + 2.3% + 5.4%

CANOLA OIL
Bran Oil Blend

Blend(75%)

1.37

Bran Oil Blend

Blend(75%)

12.02

+ 1.04

Bran Oil Blend

Blend(75%)

12.2%

2.0%

47.3%

31.7%

2.8%

4.1%

100%

14.1%

+ 8.2%

CANOLA OIL
3.NUSUN/RICE BRAN OIL BLEND

1.PV Test

Control High Oryzanol Rice Bran Oil Blend

Product Nusun 100% Blend(25%) Blend(50%)

Nusun 0.70 1.68 1.91

2.OSI Test

Control High Oryzanol Rice Bran Oil Blend

Product Nusun 100% Blend(25%) Blend(50%)

Nusun 8.93 9.04 10.82

Improvement VS. Controll Err:522 Err:522

3.Fatty Acid Compositon

Control High Oryzanol Rice Bran Oil Blend

Nusun 100% Blend(25%) Blend(50%)

Palmitic Acid(C16-0) 4.5% 6.7% 9.6%

Stearic Acid(C18-0) 3.5% 3.2% 2.8%

Oleic Acid(C18-1) 60.6% 56.7% 51.4%

Linoleic Acid(C18-2) 29.3% 30.7% 32.6%

Linolenic Acid(C18-3) 0.1% 0.4% 0.7%

Other 2.0% 2.4% 3.0%

100% 100% 100%

Total (C:16:0/C18:0) 8.0% 9.8% 12.4%

Saturated Fat Increase(C16:0/C18:0) + 1.8% + 4.4%

NUSUN
ce Bran Oil Blend

Blend(75%)

1.39

ce Bran Oil Blend

Blend(75%)

11.33

Err:522

ce Bran Oil Blend

Blend(75%)

12.3%

2.4%

46.6%

34.3%

1.0%

3.5%

100%

14.7%

+ 6.7%

NUSUN
4.HO SAFFLOWER OIL/RICE BRAN OIL BLEND

1.PV Test

Control High Oryzanol Rice Bran Oil Blend

Product Safflower 100% Blend(25%) Blend(50%)

HO safflower Oil 0.65 1.29 1.33

2.OSI Test

Control High Oryzanol Rice Bran Oil Blend

Product Safflower 100% Blend(25%) Blend(50%)

HO safflower Oil 11.68 12.81 12.37

Improvement VS. Controll + 1.13 + 0.69

3.Fatty Acid Compositon

Control High Oryzanol Rice Bran Oil Blend

Safflower 100% Blend(25%) Blend(50%)

Palmitic Acid(C16-0) 5.1% 7.9% 9.9%

Stearic Acid(C18-0) 2.2% 2.1% 2.1%

Oleic Acid(C18-1) 76.2% 66.4% 59.2%

Linoleic Acid(C18-2) 14.7% 20.8% 25.2%

Linolenic Acid(C18-3) 0.1% 0.4% 0.7%

Other 1.8% 2.4% 2.9%

100% 100% 100%

Total (C:16:0/C18:0) 7.3% 10.0% 12.0%

Saturated Fat Increase(C16:0/C18:0) + 2.8% + 4.8%

HO Safflower Oil
ce Bran Oil Blend

Blend(75%)

1.17

ce Bran Oil Blend

Blend(75%)

12.71

+ 1.03

ce Bran Oil Blend

Blend(75%)

12.3%

2.0%

50.8%

30.4%

0.9%

3.5%

100%

14.4%

+ 7.1%

HO Safflower Oil