Scribd
Upload
21 views

NPC Prac Report 1

1. The document describes a practical experiment to extract piperine from black pepper. 2. 10g of black pepper seeds were ground and placed in a soxhlet extractor with 200mL of methanol for 1 hour. 3. The extracted methanol solution was filtered and evaporated to obtain 1.18969g of crude piperine extract, yielding an 11.9% extraction of the piperine from the original black pepper seeds.

Uploaded by

saf
Copyright
© © All Rights Reserved
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
21 views

NPC Prac Report 1

1. The document describes a practical experiment to extract piperine from black pepper. 2. 10g of black pepper seeds were ground and placed in a soxhlet extractor with 200mL of methanol for 1 hour. 3. The extracted methanol solution was filtered and evaporated to obtain 1.18969g of crude piperine extract, yielding an 11.9% extraction of the piperine from the original black pepper seeds.

Uploaded by

saf
Copyright
© © All Rights Reserved
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
You are on page 1/ 2

Name: Soh Zhi Hui

Module group: MC1202


Module: CL2853 Natural Product Chemistry

Practical 1:
Extraction of piperine from black pepper

Objectives
1. To isolate the piperine compound from black pepper
2. To learn isolation techniques

Introduction
Piperine, a solid insoluble in water, is a weak base which has a burning aftertaste. Piperine is
classified under the vanilloid group of compounds, together with capsaicin,a pungent substance
found in chilli peppers. The trans-trans stereoisomer of 1-piperoylpiperidine, piperine is also
known as (E, E)-1-piperoylpiperidine or (E, E)-1-[5-(1, 3-benzodioxol-5-yl)-1-oxo-2,4-
pentdienyl]piperidine, as represented by the chemical structure below:

O
O

Piperine

Procedure
10 g of black pepper seeds were grounded into powder form with motor and pestle.


The grounded black pepper seeds were then transferred to the cellulose thimble and placed into
the soxhlet extracting flask.


200 mL of methanol was measured with a 250mL measuring cylinder and then transferred to a
250 mL round bottomed flask. Boiling chips were added into the round bottomed flask.

A reflux reaction was set up, and the extraction was carried out for an hour.


The extracted methanol solution was allowed to cool to room temperature with the help of an ice
bath. The cooled solution was then filtered into another 250 mL round bottomed flask,which has
been pre-weighed.


Methanol was removed from the filtrate using the rotary evaporator, and crude methanol was
obtained.


The round bottomed flask with the crude methanol extract was weighed. The weight of the crude
methanol extract was calculated and recorded.

Answers pg. 8

Black pepper seeds 10.0306 g


Crude MeOH extract 1.18969 g
% yield of crude MeOH extract 11.9 %
Colour of crude MeOH extract Pale yellow precipitate

Calculation:

Actual weight of black pepper seeds = 10.0306 g

Weight of empty 250 mL round bottomed flask = 168.3799 g

Weight of 250 mL round bottomed flask with crude methanol extract = 169.56959 g

Weight of crude methanol extract = 169.56959 g 168.3799 g


= 1.18969 g
weight of crude methanol extract obtained
percent yield = 100%
weight of black pepper seeds
sdlnclkxszklc= (1.18969 g / 10.0306 g) 100%
= 11.9 %

You might also like