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Biotech Syllabus

Unit I discusses principles of immunology including antigen-antibody reactions and their applications in vaccine standardization and storage. Unit II covers recombinant DNA technology techniques for producing recombinant DNA and monoclonal antibodies, and their applications. Unit III examines screening soil for antibiotic-producing organisms, fermentor design and operation, and isolation of mutants. Unit IV discusses microbial transformation reactions, organism selection, and process improvements for biotransformation, especially of steroids. Unit V focuses on enzyme immobilization techniques, advantages and limitations of immobilization, and biotechnological production of immobilized enzymes for pharmaceutical applications.

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139 views

Biotech Syllabus

Unit I discusses principles of immunology including antigen-antibody reactions and their applications in vaccine standardization and storage. Unit II covers recombinant DNA technology techniques for producing recombinant DNA and monoclonal antibodies, and their applications. Unit III examines screening soil for antibiotic-producing organisms, fermentor design and operation, and isolation of mutants. Unit IV discusses microbial transformation reactions, organism selection, and process improvements for biotransformation, especially of steroids. Unit V focuses on enzyme immobilization techniques, advantages and limitations of immobilization, and biotechnological production of immobilized enzymes for pharmaceutical applications.

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meet2abhay
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BOP-482

PHARMACEUTICS-X
PHARMACEUTICAL BIOTECHNOLOGY
Unit I
Immunology and immunological preparations: Principles, antigen and haptens, immune system,
cellular and humoral immunity, immunological tolerance, antigen-antibody reactions and their
applications, standardization and storage of vaccine.

Unit II
Recombinant DNA technology: A brief introduction to genetic engineering and techniques,
production of r-DNA and their application, development of hybridoma for monoclonal antibodies
and their application, protoplast fusion and biotechnological production of products such as
Insulin and Somatotropin.

Unit III
Antibiotics: Screening of soil for organisms producing antibiotics.
Fermentor: Basic design, control of different parameters and application.
Isolation of mutants and factors affecting mutation.

Unit IV
Microbial transformation: Introduction, types of reactions mediated by microorganisms,
selection of organisms, methodology of biotransformation, process improvements with special
reference to steroids.

Unit V
Enzyme immobilization: Sources of enzymes, techniques of immobilization of enzymes and cell,
advantages and limitation of immobilization, application of immobilization in pharmacy.
Biotechnological production and pharmaceutical application of enzymes such as pen -
galactosidase, amylases and proteases.
BOP-482P
PHARMACEUTICS-X
PHARMACEUTICAL BIOTECHNOLOGY PRACTICAL

1. Estimation of protein in given sample.


2. Production of protoplast fused cells by chemical method.
3. Production of protoplast fused cells by mechanical method.
4. Estimation of immunological reaction (blood group etc.).
5. Assay of antibiotics.
6. Screening of soil for antibiotic producing microorganisms.
7. Immobilization of drug.
8. Immobilization of enzyme.
9. Immobilization of cell.
10. Protein estimation by gel electrophoresis.
11. Isolation of enzymes from natural sources.

BOOKS RECOMMENDED
1. CBS Publishers and Distributors, New Delhi.
2. Vyas S.P. and Dixit V.K., Pharmaceutical Biotechnology, CBS Publication, New Delhi.
3. Kieslich K. , Biotechnology, Verleg Chernie, Switzerland.
4. Standury P.F.,Whitaker A. & Hall S.J. ,Principles of Fermentation, Aditya Book Private
Limited, New Delhi.
5. Crueger W. & Crueger A, Biotechnology- A Textbook of Industrial Microbiology, Panima
Publishing Corporation, Delhi.

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