Offline Sorter™

Plexon’s Offline Sorter™ (OFS) is the most recognized and trusted offline spike sorting software in the industry today with
1,700+ publications specifically citing the use of OFS as part of their methods. OFS accepts file types from many data
acquisition companies and software programs such as those listed in the
technical specifications table—including, Plexon’s advanced PL2™ file type.

OFS v4 is a powerful, easy-to-use tool for viewing and classifying action

potential waveforms (spikes) previously collected from single electrodes,
stereotrodes and tetrodes. Spikes can be displayed as points in either
2D or 3D feature space, where a variety of manual, semi-automated, or
fully automated clustering techniques can be applied in order to classify
(sort) the spikes. OFS can also perform spike extraction on continuously-
recorded neural data using a variety of different thresholding methods.
OFS allows verification of sorting through a variety of displays, and can
calculate cluster separation statistics and sort quality metrics. The sorted
spikes can be exported in several formats for subsequent analysis.

Powerful Waveform Viewing and Sorting

O
 verlapping waveform analysis Raster displays of spikes and continuous data
Printable Sort Summary View with export to PowerPoint®
 aveform features and statistics can be easily exported to
W
MATLAB®, Excel® or text file
Export of sorted data to NeuroExplorer®
 ave and recall particular sorting instances for a selected
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channel (useful for comparisons)

Time Segmentation, Adaptive Sorting

Methods, and Time-dependent Analysis
 ime Segments can be named and individually colored
T
and toggled on/off, and can be saved/restored as
NeuroExplorer Intervals
Ability to display arbitrary (compatible) combinations Interval Selection tool can create Time Segments
of continuous and spike data for a channel together in
the Timeline View which replaces the need to associate Ability to load multiple PL2 files simultaneously
channels during loading of PLX files or during channel  ivide each file into time segments and view and sort
D
remapping waveforms from each time segment separately
Support for multiple spike Sources  lusters vs Time View to show how clusters evolve as a
C

Manual cluster selection in 3D feature space using principal function of time
component projections, voltage slices in time, or more than  ort Quality View vs Time Segment Graph View to display
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20 other waveform features such as peak, valley, full-width how the sort quality changes through the file
at half maximum, etc.
 daptive template sorting, with the templates changing
A
Box sorting as a pair of time-level windows over time to follow the waveform as it evolves
Waveform selection in time-voltage space  emplates vs Time View to display how the template
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Template matching algorithm adapted through the file
Tetrode and Stereotrode waveform extraction and sorting
using any method
Unit cross-correlograms and ISI plots
Waveform density plots in 2D or 3D in any feature space
Waveform alignment
Interval invalidation and artifact removal

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For more information email [email protected] or call +1-214-369-4957 www.plexon.com
OFSDS0001h
  hreshold Scan Graph View allows examining SNR and
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Semi-Automatic and Automatic Sorting number of spikes as a function of threshold position for
continuous data
Up-sampling of continuous signals, with linear or spline
interpolation
Fast Fourier Transform (FFT) View of continuous data
Single and Dual Thresholds for spike detection
Waveform detection and extraction now appropriately
handles differences in voltage scales between spike
waveform segments and continuous spike data

Improved User Interface, Displays, and

Performance
Customizable contents of the Sort Summary View
Includes L-Ratio and Isolation Distance sort quality metrics
Read times for PL2 files for single channels are up to 100s
Includes Lines and Bands as sort methods or 1000s times faster
Scan modes, including ability to scan using different Enhanced Timeline View showing both continuous and
random initial seed clusters waveform data simultaneously with a resizable splitter
Option to use standard deviation or MAD to calculate fit Mouse-over time and voltage readings in the Waveform
tolerances or band fit tolerances View and Continuous Data View
Enhanced automatic sorting methods with systematic More information related to gains and voltage ranges in
scanning through parameter space and graphs displaying the Channel Parameters View and File Summary View
the sort quality metric as a function of the parameters that
Ability to select the next/previous channel, unit, waveform
were varied; select the best sorting according to a selected
or time segment on main menu
sort quality metric
Performance enhancements for loading data files faster
 utomatic sort scan results for each channel can be saved
A
to and loaded from SCAN files. This, together with the Available as a true 64-bit executable for faster processing
new batch mode scan commands, will allow the running of large data files
of certain sorting scans overnight, followed by manual
reviewing and selection of the most appropriate sort for Technical Specifications
each channel OFS Features Specifications and Options
Automatic cluster selection in feature space using Valley File types Plexon (.pl2, .plx), NeuroExplorer (.nex),
Seeking or T-Distribution Expectation Maximization (E-M) supported Alpha Omega (.map, .mpx), Axion Biosystems
(.spk, .raw), Cyberkinetics/Blackrock Microsystems
Semi-automatic cluster selection in feature space using (.nev, .ns5), CED Spike-2 (.smr), DataWave (.uff, .dat,
K-Means or Standard E-M .cut, .act), Neuralynx (.ntt, .nst, .nse, .ncs, .nvt), Multi
Channel Systems (.mcs, .mcd), Panasonic (.med),
Enhanced Batch Mode Processing for Neuroshare (.nsn), Data Translations (.dcf), generic
binary continuous data, and other formats supported
Running Sorting Algorithms Overnight via Neuroshare

 atch mode commands for scanning through automatic

B Electrode support Single, stereotrode and tetrode
sorting parameters Spike detection Voltage threshold, Signal Energy, Nonlinear Energy
methods and Signed Signal Energy
Edit batch files with a user-selected editor
Spike sorting - Manual methods: Boxes, Lines, Bands, Waveform
Record of previous batch file locations methods Crossing and Contours
- Semi-Automatic methods: Templates, K-Means and
Quick Batch Reference showing all batch commands Standard E-M (Expectation Maximization)
Log file resulting from the latest batch run shown on menu - Automatic methods: Valley-Seeking, T-Distribution
E-M and Scanning methods
Enhanced Continuous Data Handling and Export options MATLAB, Excel, text file or NeuroExplorer
Spike Detection Computer CPU Pentium® III or better
Ability to apply high-cut filtering to continuous channels Platform Windows® 7

D
 igital referencing
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 ignal-to-Noise ratio (SNR) computation

PLEXON®, the five-line symbol, CereStage™, CineCorder™, CineLAB®, CineLyzer™, CinePartner™, CinePlex®, CineTracker™, CineTyper™, DigiAmp™, MiniDigi™, Offline Sorter™, OmniPlex®,
PL2™, PlexBright®, PlexDrive™, PlexStim™, Radiant™ and RapidGrid™ are registered and unregistered trademarks of Plexon Inc, Dallas, Texas, USA. ©2016 Plexon Inc.
All rights reserved. Other product and company names mentioned are trademarks of their respective owners.

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