Micron User Guide
Micron User Guide
User Guide
Document control number: ZP-PKG-0156[D]
March, 2010
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Micron
Table of Contents
1. Micron Features ........................................................................... 1
Micron System Requirements ........................................................................ 1
4. Operation ................................................................................... 13
Getting Started............................................................................................. 13
Running Micron ............................................................................................ 14
Adjusting the Image ..................................................................................... 14
Resolution .............................................................................................. 15
Other Adjustments ................................................................................. 15
Capturing an Image ..................................................................................... 16
Returning to Live Image ......................................................................... 16
Annotation and Measurement ...................................................................... 16
Submenu Options .................................................................................. 18
Select All ................................................................................................ 19
Saving an Image .......................................................................................... 20
Printing an Image ......................................................................................... 20
Print Previewing an Image ........................................................................... 21
Auto Saving an Image .................................................................................. 22
Calibration.................................................................................................... 23
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Micron
7. Troubleshooting ........................................................................ 44
More Help .................................................................................................... 45
9. Warranty ..................................................................................... 47
Figure
Figure 1. The main Micron window .................................................................... 14
iv
1. Micron Features
Micron is the Westover Scientific imaging software that lets you
make the best use of a camera attached to a microscope. Micron
includes the following features:
Simple and quick installation
Easy and intuitive user interface
User can quickly switch among the following display
options:
o High-resolution windowed mode, 1280 x 960
o Super high-resolution, 2048 x 1536
Can freeze images
Saves images to disk files on the PC
Recalls previously saved images for later review
Prints images
Annotates images
D:\setup
1. Select No, not this time, and click Next. This window appears:
Getting Started
Before you get started, make sure:
The camera is properly attached to the microscope.
The latest version of Micron is installed on your PC.
The USB2 cable is attached to the camera and to a USB2
port on your PC.
Note: You cannot tell by looking at a cable if it is USB or
USB2, so make sure you have the correct cable by
checking any labels.
Make sure the microscope is turned on and ready.
Place a slide or specimen on the microscope stage under the
microscope objective. Using the microscope‘s focus control knobs,
bring the image into focus.
13 Chapter 4. Operation
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Running Micron
To open Micron, double-click the Micron icon on your Desktop.
When you open Micron, a window similar to this appears:
Menu bar
Main toolbar
Image window
Annotation toolbar
Measurement
toolbar
Status bar
The Micron menus and toolbars are explained in detail later in the
menus and toolbars chapters.
14 Chapter 4. Operation
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Chapter 4. Operation 15
Micron
Capturing an Image
To capture the image on the PC screen, click either:
the capture button on the camera, if the camera has one, or
the Camera icon on Micron‘s Main toolbar:
Camera icon
Note: The Camera icon has a lighter background when you are
viewing live video:
16 Chapter 4. Operation
Micron
Change size. The eight little squares on and around the ellipse
indicate that the ellipse is the currently selected object. You can
change the size of the ellipse—and Micron will change the
measurement accordingly—by putting your cursor over one of
those eight squares. When your cursor changes to a line with two
arrows, press your left mouse button and hold the button down as
you ‗drag‘ the ellipse inward or outward to change its size.
Chapter 4. Operation 17
Micron
Submenu Options
Right-clicking on an annotation shape or text box will bring up a
context-specific submenu:
Zoom In, Zoom Out. Use your left mouse button to click on
Zoom In and make the image larger. Click on Zoom Out to restore
the image to its previous size.
For Zoom In, you can also hold down the Ctrl key while you press
the + key. For Zoom Out, hold down the Ctrl key while you press
the – key.
Reset Zoom. If you have zoomed in on an image to two or more
levels, clicking on Reset Zoom restores the image to its original
size in one step.
Cut. Click on Cut to remove the ellipse and put a copy of it in
Micron‘s clipboard. The clipboard allows you to use the Paste
Screen Item on the Edit menu and your right mouse button to place
your ellipse elsewhere in the main window.
Copy. Click on Copy to copy the ellipse. Go to another area of the
screen and click the right mouse button. A list of choices appears.
Select the Paste Screen Item choice to place a copy of the ellipse in
that area.
Clear. This feature removes the ellipse from the screen. The
ellipse is not placed on the clipboard.
Measure. This feature is available only on submenus for hollow
rectangles and ellipses. Use it to tell Micron which measurement of
your shape you want: area or dimensions.
Lock Aspect Ratio. When you select this item and then ‗drag‘ any
of the eight little squares to change the size of your ellipse, the
width and height of the ellipse remain in the same ratio to each
other. If this item is not selected and, for example, you drag the
18 Chapter 4. Operation
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Chapter 4. Operation 19
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Saving an Image
You can save a ‗snapshot‘ of the image in Micron‘s main window.
Click the Save or Save As command from the File menu, or click
the Save icon on the Main toolbar. This window appears:
Enter the file name you want for the image. You can also select the
file format the image will be saved in (for example, .png, .bmp,
.jpg, or .tif).
Note: If you choose the .png format, the system‘s current
calibrations and the image‘s overlays will also be saved in the file
for later recall and subsequent measurements.
Press the Save button to save the image.
Printing an Image
Click the Print command on the File menu, or click the Print icon
on the Main toolbar. The Print window displays the name of the
printer currently selected:
20 Chapter 4. Operation
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Chapter 4. Operation 21
Micron
When an image is captured: the two choices let you turn Auto
Save on or off for any images you capture from that point on. (You
cannot turn on Auto Save and save a captured image that is already
on your screen.)
Auto Save file naming: lets you specify the base file name and
starting index. Using the default choices, Image0000.png would be
the first image saved. The next image saved by AutoSave would be
named Image0001.png, and so forth.
Auto Save format: lets you specify Portable Network Graphic
(filename.png) or JPEG (filename.jpg) format for your files.
Portable Network Graphic is a lossless data-compression
technique in which no data is lost. In this format the
image‘s calibration and overlays are also saved for later
recall.
JPEG is a lossy compression method. A lossy conversion
process does not retain all the original information.
Auto Save folder: lets you specify which drive and folder your
files will be saved in.
22 Chapter 4. Operation
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Calibration
Note: For ease of calibration, we recommend the Westover
Scientific Video Calibration Slide (part number: VCS-6T; see
page 46 for ordering information) or any other calibration slide that
has at least 10 calibration dots.
Calibration establishes the correspondence between ‗real world‘
units and the same distance in pixels on the image. Micron
implements a wizard to make this task easy for you.
In following the directions below, select your calibration dot
carefully. For a given magnification, you want to find the one dot
on the calibration slide that fills the greatest amount of the screen
area without going beyond any of the screen edges. The largest dot
you can get on the screen will give you the most accurate
calibration. Each magnification level will, of course, have its own
‗best dot.‘
Here is an example of a ‗good‘ calibration dot:
Note: If you have not performed a calibration before, you will find
it easiest to start with your microscope‘s lowest magnification.
Chapter 4. Operation 23
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Chapter 4. Operation 25
Micron
10. Position the top and left crosshairs. Your goal is to have each
crosshair barely touch the top or left of the calibration dot‘s
curve. You do not want any of that curve to show above or to
the left of the respective crosshair.
You can use the Snap to Estimated feature, but you may also
need to manually tweak the crosshair placement with the
up/down and right/left position buttons.
11. Click Next. This window appears:
12. Position the bottom and right crosshairs. Each crosshair should
barely touch the bottom or right of the calibration dot‘s curve.
You do not want any of that curve to show below or to the right
of the respective crosshair
13. Click Next. This window appears:
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18. Click OK to save your changes. (If you do not click OK, you
will lose the calibration you have just added.)
Repeat this calibration process for your microscope‘s other
magnification levels. The dot you select will be different for each
magnification level—but in each case the appropriate dot will fill
as much of the screen as possible without going past any of the
screen‘s edges.
Note: Once you have established calibrations, the name of the
selected calibration appears in the calibration box on the
Measurement toolbar. When you change magnification, you can
use the drop-down menu in this calibration box to change the
selected calibration.
Chapter 4. Operation 27
5. Micron Menus and Their Items
This chapter contains these sections, dealing with the eight Micron
menus:
File Menu
Edit Menu
View Menu
Tools Menu
Annotation Menu
Measurement Menu
Window Menu
Help Menu
File Menu
The File menu contains 11 items. (If an item has a keyboard
shortcut, that shortcut is shown in parentheses after the item‘s
name.)
New: (Ctrl+n) opens a new window.
Open: (Ctrl+o) opens a previously saved image.
Close: closes the currently displayed image.
Save: (Ctrl+s) saves the current Micron image. .
Save As: saves the current Micron image to a new or different
image file. The Save As item is discussed in detail in the Saving an
Image section.
Print: (Ctrl+p) prints the current Micron image.
Print Preview: displays the current Micron image as it will appear
when printed.
Print Setup: selects a printer and printer connection.
Edit Menu
The Edit menu contains five items. (Each item has a keyboard
shortcut, which is shown in parentheses after the item‘s name.)
Copy Image: (Ctrl+i) copies the image currently displayed on the
screen.
Paste Image: (Ctrl+v) places the most recently copied image on
the screen.
Copy Screen Item: (Ctrl+c) copies the selected items on the
screen.
Paste Screen Item: (Ctrl+v) places the most recently copied items
on the screen.
Color: allows adjustments to the image‘s brightness, contrast,
saturation, and hue.
View Menu
The View menu contains eight items:
Live Video: toggles between displaying live video and a single
captured image.
Adjust Camera: brings up a dialog box that allows you to adjust
brightness, contrast, saturation, and hue. This item is discussed in
the Adjusting the Image section.
Image at Natural Size: the image in the sensor is displayed on the
PC screen at a ratio of 1:1 or, in other words, one pixel of the
image is shown in one pixel of the screen. (Depending upon the
resolution you have selected, this item may be worded slightly
differently.)
Full Screen: displays the image using the entire area of your
screen, including the areas where the toolbars are normally shown.
Press the Esc key to return to the usual display mode.
Annotation Toolbar: shows or hides the Annotation toolbar.
Measurement Toolbar: shows or hides the Measurement toolbar.
Main Toolbar: shows or hides the Main toolbar.
Status Bar: shows or hides the Status bar.
Tools Menu
The Tools menu contains three items:
Microscope: preset for USB2 models; no adjustments needed.
Auto Save: automatically saves any captured image to file at the
instant the image is captured (before any annotations or other
changes are added to the image). The Auto Save feature is
discussed in the Auto Saving an Image section.
Live Overlay: clicking on this item brings up the Live Overlay
dialog box:
This dialog box lets you superimpose a grid on the screen. You can
select the number of rows and columns, and the color and
thickness of the lines in the grid.
The Bitmap File option lets you call up a saved file and overlay it
on top of your image. This feature is useful for video comparators.
In this feature:
The screen overlay must be a Windows bitmap (.bmp) file, so
only bitmaps or other image files—JPGs or PNGs—that can
be converted to bitmap format can be loaded. (Micron
automatically converts a .jpg or .png file loaded in this feature
to bitmap.)
If the bitmap file includes a transparency band (alpha band),
the transparency information is applied on a per-pixel basis. If
the information is not supplied, the dominant color is taken as
the transparent background.
Micron scales the bitmap to match the image size, so for best
results set the bitmap to match the Micron 4:3 aspect ratio. For
example, if you are creating an overlay file with Microsoft
Paint, select Image > Attributes and set the Width to 1280 and
the Height to 1536.
Annotation Menu
The Annotation menu contains 15 items that let you add text,
arrows, and lines to your captured images. You can also add
geometric shapes to outline or enclose features you want to draw
attention to.
Note: When you have Live Video selected, only the Default Color
item is enabled on this menu. Similarly, even when you are in
capture mode and Live Video is turned off, only the Default Color
item is enabled if the Show Annotations item is not checked.
Here is a description of the Annotation menu‘s 15 items:
Erase All Annotations!: choose this menu item to erase all
annotations from the screen. This item does not erase any
annotations from the file if the image has been saved to a file in the
.png format.
Show Annotations: uncheck this item to temporarily hide any
annotations you have made on the image.
Default Color: lets you select the color for any annotation you add
using an item on the Annotation menu.
Select Annotations and Measurements: check this item to enter
the select-annotation-and-measurement mode. In annotation-and-
measurement mode you can draw a ‗rubber band‘ rectangle on the
main window to enclose and select annotations and measurements.
For more details, see the Select All section.
Arrow: to draw a line ending in an arrow, select this item, and
move your cursor to the main window. Place the cursor where you
want the line to begin, and press and hold down the left mouse
button as you drag the line to where you want it to end with an
arrow. Release the mouse button.
Line: to draw a straight line, select this item, and move your cursor
to the main window. Place the cursor where you want the line to
begin, and press and hold down the left mouse button as you drag
the line to where you want it to end. Release the mouse button.
Text: to insert text, select this item, and move your cursor to the
main window. Place the cursor where you want the text to begin,
and press and hold down the left mouse button as you drag the
cursor to where you want the text to end. Release the mouse
button. Type the text into the area you have designated. If the text
exceeds the box, you can stretch the box.
Measurement Menu
Among other functions, the Measurement menu lets you show,
select, and add geometric shapes to your images. After you create a
geometric shape, Micron calculates and displays its measurement.
Note: When you have Live Video selected, only a few items are
enabled on this menu. Similarly, even when you are in capture
mode and Live Video is turned off, only a few items are enabled if
the Show Measurements item is not checked.
Detailed instructions for using the drawing items—such as Line
Length, Circle Radius, and Polygon Area—are in the descriptions
of the corresponding items on the Annotation menu, earlier in this
chapter.
The Measurement menu contains 17 items:
Calibration: sets up calibration using a known standard. This item
is discussed in detail in the Calibration section.
Units and Scale Rulers: options for measurement units (microns,
inches, or millimeters) and for the vertical and horizontal scale
rulers.
Preferences: allows you to specify what values are measured:
1. Adjust your cursor to a size smaller than the samples you want
to select.
2. Click Next. The main window reappears.
sample
Every area in the main window that contains the color you selected
is now outlined, and Micron has calculated and displayed its area
measurement.
Window Menu
If you have two or more instances of Micron running at the same
time, this menu lists the number of screens, with a checkmark
beside the currently displayed screen.
Help Menu
The Help menu has four items:
Help Topics: displays this dialog box:
Enlarge the window and use the Contents tree or Search option to
obtain help on any of the listed topics.
Troubleshoot Startup Error Message: lists several error
messages that might appear when you start Micron, and suggests
possible solutions to each one.
User Guide: brings up this User Guide if you have an
application—usually Adobe Reader—on your computer that can
read pdf files (If you don‘t have a reader, perhaps you are looking
at a copy of this User Guide printed from another computer.)
Note: You can get a free copy of Adobe Reader by downloading it
from this URL:
http://www.adobe.com/products/acrobat/readstep2.html
Note: to select any toolbar icon, put your cursor on it, and click the
left mouse button.
Main Toolbar
The Main toolbar lets you quickly open images, save images, and
capture live images, among other functions.
Note: Most of the icons on the Main toolbar are identical or similar
to items on the File, Edit, and View menus—and they are
discussed in more detail on those menus.
The Main toolbar contains 10 icons:
Saves the current Micron image. If the image does not have a
title, the Save As dialog box appears. For more detail, see the
Saving an Image section.
Copies the currently selected screen item to the clipboard.
Prints the current Micron image. For more details, see the
Printing an Image section.
Displays the current Micron version and other information
about the software.
Crops the current Micron image and places the cropped image
in a new window.
Adjust Camera controls the camera‘s brightness, contrast, and
color display. For more details, see the Adjusting the Image
section.
Toggles live video on and off.
Annotation Toolbar
The Annotation toolbar, located toward the bottom of the main
window, just above the Measurement toolbar and Status bar, lets
you add text, arrows, and lines to your captured images. You can
also add geometric shapes to outline or enclose features you want
to draw attention to.
Note: When you have Live Video selected, none of the Annotation
toolbar icons are available.
Note: Most of the icons on the Annotation toolbar are identical to
items on the Annotation menu. Those items are discussed in more
detail in Annotation Menu section.
The Annotation toolbar contains 13 icons:
Measurement Toolbar
The Measurement toolbar, located toward the bottom of the main
window, just above the Status bar, lets you show, select, and add
geometric shapes to your images. After you create the geometric
item, Micron calculates and displays its measurement.
Note: Most of the icons on the Measurement toolbar are identical
to items on the Measurement menu. Those items are discussed in
more detail in the Measurement Menu section.
The Measurement toolbar contains 10 icons and a calibration box:
Status Bar
The Status bar is located at the bottom of the main window.
When your cursor is over a toolbar icon or a menu item, the left
part of the status bar displays a brief description of that icon or
item.
If your cursor is not over an icon or item, the Status bar usually
displays a Ready status, meaning that Micron is ready to perform
the next procedure.
The Count section of the Status bar, located in the right-middle
section of the bar, tells you how many items are on your screen at
any given time.
The section on the far right of the Status bar indicates if you have
Caps Lock, Num Lock, or Scroll Lock activated.
Chapter 7. Troubleshooting 44
Micron
More Help
Other Micron USB2 troubleshooting guides can be found in the
Help menu:
under Troubleshoot Startup Error Message.
under Help Topics > Troubleshooting USB2 Video Problems.
If you still cannot solve the problem, contact Westover Scientific
technical support for assistance. Our contact information is on
page 46.
Chapter 7. Troubleshooting 45
8. Customer Support
For technical and customer support—or to place an order—please
contact Westover Scientific in one of these ways:
Westover Scientific, Inc.
18421 Bothell-Everett Hwy., Suite 150
Mill Creek, WA 98012
U.S.A.
Phone: 1-425-368-0444 or 1-866-614-4022
We are available Monday through Friday, 8:00 am to 4:00 pm
(Pacific Time in North America).
Fax: 1-425-368-0555
E-mail: [email protected]
Web: www.amgmicro.com
If you need assistance, please have the following information
readily available:
A concise description of your problem.
The type of computer you are using.
The type and version of operating system on your
computer.
Micron serial number, setup size, and version information.
(See the Help Menu section for how to find this
information.)
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10. Index
Hollow Rectangle icon, 40
Line icon, 40
A moving or dragging, 41
Polyline icon, 40
About Micron dialog box, 38 Selection (arrow) icon, 40
Adjust Camera icon. See Main toolbar, showing and hiding, 29
View menu similar to Annotation menu, 40
adjusting the camera, 14, 29 Solid Ellipse icon, 40
adjusting the image, 14 Solid Polygon icon, 41
angle Solid Rectangle icon, 40
measurement, 41 Text icon, 40
measuring shallow angle, 34 Toggle icon, 40
measuring wide angle, 34 annotations
annotating a captured image, 16 erasing, 31
annotation area or dimensions, 17, 18, 34, 41
change size of, 17 arrow, 31, 40
Annotation menu, 16, 17, 31 adding to image, 31
Arrow item, 31 auto-create, 35
Default Color item, 31 AutoSave. See also Tools menu
Erase All Annotations item, 31 AutoSave dialog box, 22
Freehand Line item, 32 AutoSave item, 22
Hollow Ellipse item, 32 autosaving an image, 22, 30
Hollow Rectangle item, 32
Line item, 31
Polygon item, 32 B
Polyline item, 32
Select Annotations and Measurements bee knee, 17
item, 31 bitmap file, 30
Show Annotations item, 31
Solid Ellipse item, 32
Solid Polygon item, 32 C
Solid Rectangle item, 32
Text item, 31 calibration, 22, 23, 25, 33, 41
Annotation toolbar, 16, 17, 34, 40 available listings, 27
Arrow (drawing) icon, 40 clicking OK to save, 27
effect of live video, 40 crosshairs, 26
Freehand Line icon, 40 dot, 23, 25, 27
Hollow Ellipse icon, 40 magnification, 23
Hollow Polygon icon, 41 Measurement menu item, 23
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Westover Scientific Micron
Index 49
Micron
I JPEG format, 22
image
adding a circle, 34 L
adding a freehand line, 32
adding a line, 31, 34 line
adding a polygon, 32 adding to image, 31, 34
adding a polyline, 32 freehand, 32, 40
adding a rectangle, 32 measurement, 41
adding a solid polygon, 32 straight, 31
adding an arrow, 31 line segment, 40
adding an ellipse, 32, 34 list of figures, iv
adjusting, 14 list of files recently viewed, 29
annotating, 16 list of tables, iv
autosaving, 30 Live overlay, 30
Autosaving, 22 dialog box, 30
brightness, 15 live video, 16, 29, 31, 33, 40
capturing, 16 effect on Annotation menu, 31
closing, 28 effect on Measurement menu, 33
color saturation, 15 toggling on and off, 40
contrast, 15
copying, 29
cropping, 40 M
e-mailing, 29
Main toolbar, 16, 20, 39
frame rate, 15
Adjust Camera icon, 14, 40
full size, 29
Camera icon, 16, 40
hue, 15
background, 16
live video, 16 Copy icon, 39
measuring, 16 Crop icon, 40
measuring an angle, 34 New icon, 39
natural size, 29 Open icon, 39
opening, 28 Paste icon, 39
overlays, 21 Print icon, 20, 39
pasting, 29
50 Index
Westover Scientific Micron
Index 51
Micron
52 Index
Westover Scientific Micron
V email, 45
webpage, 45
vertical ruler, 26, 33 Westover Scientific Video Calibration
VGA drivers, 43 Slide, 23
video capture settings Width dialog box, 19
advanced options, 16 window
View menu, 14, 29 new, 28
Adjust Camera item, 29 Window menu, 38
Annotation Toolbar item, 29 windowed area
Full Screen item, 29 selecting, 15
Image at Natural Size item, 29 Windows
Live Video item, 16, 29 2000, 1
Main Toolbar item, 29 XP, 1
Measurement Toolbar item, 29
Status Bar item, 29
Z
W zooming
Ctrl key, 18
Westover Scientific in and out, 18
contacting, 45
Index 53