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Mosmol/Kg N X Mmol/L Mosmol/Kg (N X MG/DL X 10) ÷ Mol WT Mosmol/Kg (N X Meq/L) ÷ Valence

The osmotic pressure of a solution depends on the number of particles per unit volume of solvent, not the type, weight, or valence of the particles. Osmolality is measured in milliosmols per kilogram and relates to the number of osmoles of solute per kilogram of solvent. When a compound dissociates into multiple particles, like NaCl dissociating into Na+ and Cl-, each mole contributes more than one osmole to the solution.

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81 views2 pages

Mosmol/Kg N X Mmol/L Mosmol/Kg (N X MG/DL X 10) ÷ Mol WT Mosmol/Kg (N X Meq/L) ÷ Valence

The osmotic pressure of a solution depends on the number of particles per unit volume of solvent, not the type, weight, or valence of the particles. Osmolality is measured in milliosmols per kilogram and relates to the number of osmoles of solute per kilogram of solvent. When a compound dissociates into multiple particles, like NaCl dissociating into Na+ and Cl-, each mole contributes more than one osmole to the solution.

Uploaded by

Miguel Xanadu
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© © All Rights Reserved
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The osmotic pressure generated by a solution is proportional to the number of particles per unit

volume of solvent, not to the type, valence, or weight of the particles.

The unit of measurement of osmolality is the osmole. The term relates to osmosis and is typically
used for osmotically active solutions. The osmolality of a solution is the number of osmoles of
solute per kilogram of solvent. One osmole (Osmol) is defined as one gram molecular weight (1
mole) of any non-dissociable substance (such as glucose) that contains 6.02 x 10 23 particles and
contributes to a solution’s osmotic pressure. In the relatively dilute fluids in the body, the
osmolality is measured in milliosmols (one-thousandth of an osmole) per kilogram of water
(mOsmol/kg). Osmolarity is similar but is defined as the number of osmoles (or mOsmol) per liter
of solvent. Since most solutes are measured in the laboratory in units of millimoles per liter,
milligrams per deciliter, or milliequivalents per liter, the following formulae must be used to
convert into mOsmol/kg:

mOsmol/kg = n x mmol/L

mOsmol/kg = (n x mg/dL x 10) ÷ mol wt

mOsmol/kg = (n x mEq/L) ÷ valence

where n is the number of dissociable particles per molecule. When n = 1, as for Na +, Cl-, Ca2+, urea,
and glucose, 1 mmol/L equals 1 mOsmol/kg. If, however, a compound dissociates into two or
more particles, 1 mmol/L will generate an osmotic effect greater than 1 mOsmol/kg. For example,
a solution of 1 mol/L NaCl corresponds to an osmolarity of 2 osmol/L. The NaCl salt particle
dissociates fully in water to become two separate particles: a Na + ion and a Cl- ion. Therefore, each
mole of NaCl becomes two osmoles in solution, one mole of Na+ and one mole of Cl-(1).

In the laboratory, the osmotic concentration of a solution is measured not as an osmotic pressure
but according to other properties of solutions (known as colligative properties) such as their ability
to depress the freezing point or elevate the boiling point of water. Solute-free water freezes at 0°
C. If 1 Osmol of any solute (or combination of solutes) is added to 1 kg of water, the freezing
point of this water will be depressed by 1.86° C. This observation can be used to calculate the
osmotic concentration of a solution. For example, the freezing point of the plasma water is
normally about -0.521° C. This represents an osmolality of 0.280 Osmol/kg (0.521/1.86) or
280 mOsmol/kg.

Importantly, only solutes that cannot cross the semipermeable membrane separating two
compartments generate an effective osmotic pressure. Thus, urea, which can cross the cell
membrane, does not contribute to osmotic pressure but will be measured as part of the plasma
osmolality by freezing point depression. There is therefore a difference between the total
osmolality and the effective osmolality of a solution, with the latter being determined only by
osmotically active solutes (such as Na+ and K+ across the cell membrane).

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