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Flow Chart of Procedures New

The document outlines the process for conducting a study on the effects of Ipomoea batatas L. (sweet potato) leaf extract on blood formed elements in albino rats. It includes: 1) Flow charts showing the extraction of leaf extracts at different concentrations and administration to test groups of rats along with a negative control group given chloramphenicol. 2) Tables showing the experimental phases, dosage categories, control/treatment groups, and timetable/schedule for the study. 3) A Gantt chart laying out the timeline for study activities and 4) A budget table listing the required materials and their costs for a total projected budget of PHP 22,510.

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Joaquin Guillermo
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114 views6 pages

Flow Chart of Procedures New

The document outlines the process for conducting a study on the effects of Ipomoea batatas L. (sweet potato) leaf extract on blood formed elements in albino rats. It includes: 1) Flow charts showing the extraction of leaf extracts at different concentrations and administration to test groups of rats along with a negative control group given chloramphenicol. 2) Tables showing the experimental phases, dosage categories, control/treatment groups, and timetable/schedule for the study. 3) A Gantt chart laying out the timeline for study activities and 4) A budget table listing the required materials and their costs for a total projected budget of PHP 22,510.

Uploaded by

Joaquin Guillermo
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
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FLOW CHART THE ENTIRE STUDY

Assessment of Chloramphenicol Sodium Succinate in the induction of


Aplastic Anemia and Administration of Ipomoea batatas L. (Sweet potato)
leaf extract in increasing Blood Formed Elements of Rattus rattus (Albino rat)

Field sampling Experimental proper

Preparation of
Collection of I. Collection of materials
batatas L. leaf Albino rats

Extraction of I. Isolation of
batatas L. leaf Albino rats

Administration of Administration of Chloramphenicol


leaf extracts Sodium Succinate

Collection of blood
samples

Blood analysis Data analysis


FLOW CHART OF LEAF EXTRACTION AND EXTRACTION CONCENTRATION
DISTRIBUTION OF Ipomoea batatas L.

1 kilo I. batatas L. leaves

Wash with running tap water

Minced using an electric blender

Boiled for 30 min + 1L DH2O

Wring out liquid

Filter 2x w/ Whatman number 1

Filtrate (Pure Extract)

100% conc. (2ml leaf 50% conc. (1ml 25% conc. (0.5ml
extract + 0ml DH2O) leaf extract + 1ml leaf extract +
DH2O) 1.5ml DH2O)

FLOW CHART OF EXPERIMENTAL PROPER


PHASE 1

5 Albino Rats 14-21 days 709.09mg/lb

5 Albino Rats 14-21 days 909.09mg/lb


Dosage
Category
(20 Albino
Rats)
5 Albino Rats 14-21 days 1109.09mg/lb

5 Albino Rats 14-21 days 1309.09mg/lb

FLOW CHART OF EXPERIMENTAL PROPER


PHASE 2

Normal Control No administration of


(5 Albino rats) leaf extract

Control Category Positive Control 100% concentration of leaves


(25 Albino Rats) (15 Albino rats) 50% concentration leaves
25% concentration leaves

Negative Control Induced with Chloramphenicol


(5 Albino rats) S.S only

+
Group A 100% ml.
(5 Albino rats) concentration Chloramphenico
+
of leaf extract l S.S

Experimental/
50% ml.
Treatment Group B
Category concentration Chloramphenic
(5 Albino rats)
of leaf extract ol S.S
(15 Albino Rats)
+
25% ml. +
Group C
concentration Chloramphenico
(5 Albino rats)
of leaf extract l S.S

TIME TABLE

Activity Schedule
Reconnaissance Week 1
Field sampling Week 1

Experimental proper Week 2- Week 6

Report writing Week 6

Finalization of report Week 7

GANTT CHART

Activity Week Week Week Week Week Week Week


1 2 3 4 5 6 7
• Reconnaissance
• Preparation of subjects- start
of acclimatization period
• Preparation of plants
• Preparation of reagents
• Last week of adaptation
period
• Preparation of extract
• RBC, WBC and platelet
counting in subjects before
treatments
• Administration of leaf
extracts to subjects
• Continuation of
administration of leaf extracts
to subjects.
• Data analysis
• Report writing
• Finalization of Report

BUDGET
Item Pcs. Unit price Total

Rat 55 120 8250


Container 11 300 3300
Gavage Feeder
Bottle feeder 22 80 1760
Rat chow 50 kg 40 p/kg 2000
Saw dust 100 20 2000
Reagents
RBC 200 ml
WBC 200 ml
Plt. ReesEcker/ 1 % Ammonium 200 ml
Oxalate
Micropipette 2
RBC 2 200/ pc 400
WBC 2 200/ pc 400
Counting chamber 2 2000/pc 4000
Counter 3
Microtainer tube (EDTA) 4 packs
Small weighing scale 1 400/pc 400
Total 22, 510

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