8/4/2018 MCQ module 1

Biochemistry
Biochemistry

MCQ module 1
October 08, 2017

Dear students, as I told you I give you all questions from yours rst module.
Enjoy :)

1. A de ciency of vitamin B12 causes:

a. Cheilosis
b. Beriberi
c. Pernicious anemia
d. Scurvy
e. Rickets
2. The vitamin required for the formation of hydroxyproline (in collagen) is
(A) Vitamin C
(B) Vitamin A
(C) Vitamin D
(D) Vitamin E
3. In new born infants phototherapy may cause hyperbilirubinemia with de ciency of
(A) Thiamin
(B) Ribo avin
(C) Ascorbic acid
 (D) Pantothenic acid
4. The Vitamin which does not contain a ring in the structure is
(A) Pantothenic acid
(B) Vitamin D
(C) Ribo avin
 (D) Thiamin
5. Consumption of raw eggs can cause de ciency of
(A) Biotin
(B) Pantothenic acid
(C) Ribo avin
 (D) Thiamin
6. Folate de ciency causes
(A) Microcytic anemia
(B) Hemolytic anemia
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(B) Hemolytic anemia

Biochemistry
(C) Iron de ciency anemia
(D) Megaloblastic anemia
7. FAD is a coenzyme for
(A) Succinate dehydrogenase
(B) Glycerol-3-phosphate dehydrogenase
(C) Sphingosine reductase
(D) All of these
8. Daily requirement of niacin is
(A) 5mg
(B) 10mg
(C) 20mg
(D) 30mg
9. Biotin is required as a coenzyme by
(A) Anaerobic dehydrogenases
(B) Decarboxylases
(C) Aerobic dehydrogenases
(D) Carboxylases
10. De ciency of vitamin C causes
(A) Beriberi
(B) Pellagra
(C) Pernicious anemia
(D) Scurvy
11. An early effect of vitamin A de ciency is
(A) Xerophthalmia
(B) Keratomalacia
(C) Prolonged dark adaptation time
(D) Follicular hyperkeratosis
12. Cholesterol is a precursor in the biogenesis of
(A) Vitamin A
 (B) Vitamin D
(C) Vitamin E
 (D) None of these
13. Sulpha drugs are antimetabolites of
(A) PABA
(B) Pyridoxine
(C) Vitamin B2
(D) Pantothenic acid
14. Transketolase activity is affected in
(A)  Biotin de ciency

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Biochemistry
(B)  Pyridoxine de ciency

(C)  PABA de ciency

(D)  Thiamine de ciency

15. Avitaminosis of what vitamin can signi cantly decrease the aminotransferase activity in
blood serum?

(A) В9, folic acid

(B) В1 thiamin
(C) В2 ribo avin
(D) В5 nicotinic acid
(E) b6 pyridoxine
16. Which ONE of the following amino acid substitutions most likely causes a change in a
protein's tertiary structure?
A. Isoleucine to arginine
B. Arginine to lysine
C. Valine to leucine
D. Threonine to serine
E. Asparagine to glutamine
17. Glycogen phosphorylase, which mobilizes glycogen for energy, requires which of the
following as a cofactor?
a) Pyridoxal phosphate
b) Tetrahydrofolate
c) Adenosyl Cobalamine
d) Coenzyme A       
18. A 54-year –old male was rushed to emergency when he collapsed in the middle of a
business meeting. Examination revealed excessive sweating and high blood pressure. ECG
chest was conclusive of Acute Myocardial infarction. Which biochemical investigation out of
the following would  be of no help in the con rmation of diagnosis?
a) Cardiac Troponins
b) Serum myoglobin
c) Lactate dehydrogenase
d) Creatine Phosphokinase-MB(CPK-MB)
19. Which out of the following co enzymes takes part in hydrogen transfer reactions in the
electron transport chain-
a) Tetrahydrofolate
b) Methyl Cobalamine
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b) Methyl Cobalamine

Biochemistry
c) Coenzyme Q
d) Biotin
20. One out of the following enzymes has absolute speci city for its substrate; choose the
correct option-
a) Urease
b) Carboxypeptidase
c) Pancreatic lipase
d)Lipoprotein lipase
21. Regarding conversion of CO2  into HCO3- in erythrocytes catalyzed by the
enzyme carbonic anhydrase, which of the following acts as a substrate(s)?
A Sunlight
B H2O
C Glucose
D H2CO3-
22. A competitive inhibitor has the following effect on a Limeweaver-Burke (double reciprocal)
Plot.
(A) It moves the entire curve to the right.
(B) It moves the entire curve to the left.
(C) It changes the y-intercept.
(D) It changes the x-intercept.
(E) It hs no effect on the slope.
23. Cyanides are extremely powerful cell’s poison which can cause death of the human body.
Which enzyme blocking in tissue respiration is basic in this action?
(A) Catalase
(B) Superoxide dismutase
(C) Cytochrome oxidase
(D) Hemoglobin reductase
(E) Glucose-6-phosphatdehydrogenase
24. A doctor, before to appoint protein's parenteral nutrition to exhausted patient, appointed to
de ne the electrophoretic spectrum of blood albumens. What physical and chemical
properties of albumens is this method based on?
(A) Optical activity
(B) Viscidity
(C) Inability to denaturation
(D) Hydrophilic properties
(E) Presence of charge
25. Patient suffers from acute pancreatitis. What medicines should be prescribed to avoid
autolysis of pancreas?
(A) Amylase
(B) Activators of proteases
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(B) Activators of proteases

Biochemistry
(C) Trypsin
(D) Chimotripsine
(E) Inhibitors of proteases
26. Which of the following compounds would be useful as a chemotherapeutic agent designed
to inhibit synthesis of purine nucleotides?
(a)Allopurinol.
(b)5- uorouracil.
(c)Methotrexate.
(d) Sulfanilamide
27. The patient has symptoms of pellagra. During interrogation it became known that
throughout long time he ate mainly corn and little meat. What became the cause of pellagra?

(A) De ciency proline in corn

(B) De ciency tyrosine in corn

(C) De ciency tryptophan in corn
(D) De ciency alanine in corn
(E) De ciency histidine in corn
28. The doctor advises to use half-cooked liver in ratio of the patient during treatment of
pernicious anemia. What vitamin presence in this product will have medical effect?

(A) Vitamin b12

(B) Vitamin В1
(C) Vitamin В2
(D) Vitamin C
(E) Vitamin Н
29. At the patient frequent bleedings from internal organs and mucous membranes are
observed. The analysis has de ned insu ciency of hydroxyproline and hydroxylysine as a part
of collagenic bres. Lack of what vitamin does bring disturbance in the process of
hydroxylation of these aminoacids?
(A) Vitamin K
(B) Vitamin A
(C) Vitamin Н
(D) Vitamin C
(E) Vitamin РР
30. Pyruvate concentration in the patient’s urine has increased 10 times from normal amount.
What vitamin de ciency can be the reason of this change:

(A) Vitamin B6
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(A) Vitamin B6

Biochemistry
(B) Vitamin A
(C) Vitamin E
(D) Vitamin C
(E) Vitamin B1
31. Enzymes catalyze reactions by
A. Increasing the free energy of the system so that the change in free energy is positive
B. Increasing the free energy of the substrate so that it is greater than the free energy of the
product
C. Changing the equilibrium constant for the reaction
D. Decreasing the free energy of activation
32. The rate-determining step of Michaelis Menten kinetics is
A. the complex formation step
B. the complex dissociation step to produce product
C. the product formation step
D. Both (a)and(c)
33. Which statement is false about covalent modi cation?
A) It is reversible.
B) It is slower than allosteric regulation.
C) It involves protein kinases.
D) Phosphorylation is a common covalent modi cation.
34. To which enzyme LDH belongs to
(A) Transferase
(B) Isomerase
(C) Lipase
(D) Oxidoreductase
(E) Hydrolase
35. Twelve hours after an acute attack of retrosternal pain in the patient presented a jump of
aspartate aminotransferase activity in his blood serum. What pathology is this deviation
typical for?
A Viral hepatitis
B Myocardium infarction
C Collagenosis
D Diabetes mellitus
E Diabetes insipidus
36. The previous diagnosis was made for the patient: myocardium infarction. Special feature
of this pathology is the substantial increase in the blood serum of activity for:
A. Catalase
B. Glucose-6-phosphate dehydrogenase
C Alpha amylase
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C. Alpha-amylase
Biochemistry
D. Arginase
E. Creatine phosphate kinase
37. The determination of this enzyme activity in the urine is diagnostic test that is used for
patients under acute pancreatitis to prove it. Name this enzyme:
A. Aldolase
B. Amylase
C. Lactatedehydrogenase
D. Creatine phosphokinase
E. Alanine aminopeptidase
38. The patient suffers from retrosternal pain from the left, the sweating and palpitation is
observed for him, too. Choose enzymes whose activity is in need to determine in the blood to
prove diagnosis: myocardium infarction:
A. AST, CPK, LDH1
B. alpha-Fetoprotein, Aldolase, CPK
C. Acidic phosphatase, LDH5, LDH4
D. amylase, alkaline phosphatase, ALT
E. ALT, aldolase, LDH4
39. Special growing factor is in need for tumor cell. Its destruction is made proposed enzyme
used for leucosis treatment:
(A) . Asparaginase
(B) . Succinate dehydrogenase
(C) . Aspartate aminotransferase
(D) Citrate synthase .
(E) Glutaminase
40. The pharmaceutical preparation Asparaginase is used for the treatment of leucosis. Find
out its mechanism of action:
A. Protein synthesis stimulation
B. Asparagine synthesis
C. Glutamine synthesis
D. Glutamine desruction
E. Asparagine degradation
41. Milk digestibility is improved by using
A. RNase
B. lactase
C. β-amylase
D. none of these
In the gure below, the solid line shows the dependence of the rate of an enzyme catalyzed
reaction v (μmol/min) as a function of the substrate concentration [S] (mM).  Also shown
(dotted line) is the dependence of the rate on substrate concentration in the presence of an
inhibitor (at a concentration of 2 mM) Questions 42 44 refer to this gure
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inhibitor (at a concentration of 2 mM).  Questions 42-44 refer to this gure.
Biochemistry

42 Regarding the activity of the enzyme in the absence of the inhibitor (solid line), which of the
following conclusions is correct?
A) The Vmax of the enzyme is 0.5 μmol/min.
B) The Km of the enzyme is 2 mM.
C) When [S] = 2 mM, v = 2 μmol/min.
D) Vmax/Km = 0.5 μmol/min (mM)-1
E) When [S] = 1 mM, v = 1 μmol/min.
43 In the presence of the inhibitor (dotted line), the Km' (apparent Km) of the enzyme, in units
of mM, is:
A) 0.25
B) 0.5
C) 1.0
D) 2.0
E) 4.0
44 On the basis of the graphs shown above, which of the following statements correctly
describes the nature of the inhibitor/inhibition?
A) The inhibitor is competitive.
B) The inhibitor is non-competitive.
C) The inhibitor acts as a positive effector.
D) The inhibitor is likely to bind to a site distinct from the active site of the enzyme.
E)  The inhibitor is likely to act as an allosteric effector.
45. Sulfonamide preparation was prescribed for the patient suffered from sore throat.
Antimicrobial effect of this preparation is associated with the damage of folic acid synthesis.
Name compound that competes with sulfonamide to be attach to active center of enzyme:
A. Glutamic acid
B. Citric acid
C. Ubiquinone
D. Succinate
E. Para-amino benzoic acid

I. Connect vitamin and sigh of its de ciency

1. B1
A. Decreased vision in half-dark
2. B2
B Symmetric dermatitis
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B. Symmetric dermatitis

Biochemistry
3. A
C. Increased homocysteine, anemia
4. PP
D. Redness, swelling of the mouth corners
5. B12
E. Increased pyruvate in blood

II. Which vitamin de ciency is associated with Neural tube defects?

III. Patient who smokes everyday ½  pack of cigarettes suffers from shortness of breath. Blood
test revealed decrease in functional protein, which are produced by liver (lipoprotein lipase,
pseudocholine esterase activities). What may be reason of liver problem and embolia? What
drugs should be prescribed?

1. In adults, a severe de ciency of vitamin D causes?

a. Night blindness
b. Osteomalacia
c. Rickets
d. Osteogenesis imperfecta
e. Osteopetrosis
2. Which of the following is not a mechanism for altering the ux of metabolites through the
rate-determining step of a pathway?
A. Allosteric control of the enzyme activity
B. Block active sites
C. Genetic control of the enzyme concentration
D. Covalent modi cation of the enzyme
3. Ribo avin de ciency causes
(A) Cheilosis
(B) Loss of weight
(C) Mental deterioration
(D) Dermatitis
4. Pantothenic acid is a constituent of the coenzyme involved in
(A) Decarboxylation
(B) Dehydrogenation
(C) Acetylation
(D) Oxidation
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(D) Oxidation
Biochemistry
5. A cofactor required in oxidative decarboxylation of pyruvate is
(A) Lipoic acid
(B) Pantothenic acid
(C) Biotin
(D) Paraaminobenzoic acid
6. Thiamin diphosphate is required for oxidative decarboxylation of
(A) α-ketoacids
(B) α-Amino acids
(C) Fatty acids
(D) All of these
7. Ribo avin de ciency can cause
(A) Peripheral neuritis
(B) Diarrhea
(C) Angular stomatitis
(D) None of these
8. Coenzyme A is required for catabolism of
(A) Leucine
(B) Isoleucine
(C) Valine
(D) All of these
9. Biotin is a coenzyme for
(A) Pyruvate carboxylase
(B) Acetyl CoA carboxylase
(C) Propionyl CoA carboxylase
(D) All of these
10. Non-competitive inhibitor of an enzyme catalyzed reaction
A. decreases Vmax
B. binds to ES
C. both (a) and (b)
D. can increase reaction velocity in rare cases
11. If an enzyme is described by the Michaelis-Menten equation, a competitive inhibitor will:
A. decrease the Km and decrease the Vmax
B. decrease the Km, but not the Vmax
C. always just change the Vmax
D. increase the Km but not change the Vmax
12. Rice polish contains vitamin:
(A) Ribo avin
(B) Niacin
(C) Thiamine
(D) Vitamin B12
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(D) Vitamin B12
Biochemistry
13. Steroidal prohormone is
(A) Vitamin A
(B) Vitamin C
(C) Vitamin D
(D) None of these
14. Patient with carcinoma has the insu ciency of vitamin В5, serotonin concentration at
blood is extremely increased. What is the reason of this state?
(A) Infringement of serotonin metabolism in organism
(B) Tryptophan mainly turns in serotonin
(C) Alimentary de ciency of vitamin РР
(D) Increased use of vitamin РР
(E) Serotonin promotes reduction of vitamin РР in an organism
15. At woman for a long time is on a diet with cleared rice, polyneuritis (illness beriberi) was
diagnosed. What vitamin insu ciency at food leads to development of this disease?
(A) Thiamin
(B) Ascorbic acid
(C) Pyridoxine
(D) Folic acid
(E) Ribo avin
16. The patient has symptoms of diarrhea, dementia and dermatitis because of wrong diet.
Lack what of vitamin causes this state?
(A) Vitamin В1
(B) Vitamin pp
(C) Vitamin В2
(D) Vitamin C
(E) Vitamin B12
17. Choose the incorrect statement about Active Site of an enzyme-
a) The active site is a three-dimensional pocket
b) The active site takes up a large part of the total volume of an enzyme
c) Substrates are bound to enzymes by multiple weak attractions
d) The speci city of binding depends on the precisely de ned arrangement of atoms in an
active site.
18. A 60-year-old chronic alcoholic was brought to the hospital with complaints of protuberant
abdomen (ascites) and edema feet. He also had history of hemorrhages.  Blood biochemistry
revealed: increased serum transaminases, low serum total proteins, albumin and a prolonged
prothrombin time. Urine analysis was normal. What could be the possible diagnosis?
a) Renal failure
b) Protein malnutrition
c) Cirrhosis of liver
d) Heart failure
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d) Heart failure

Biochemistry
19. The activities of many enzymes, membrane transporters and other proteins can be quickly
activated or inactivated by phosphorylation of speci c amino acid residues. This regulation is
called
a) Allosteric modi cation
b) Covalent modi cation
c) Induction
d) Repression
20. A 54-year–old male was rushed to emergency when he collapsed in the middle of a
business meeting. Examination revealed excessive sweating and high blood pressure. ECG
chest was conclusive of Acute Myocardial infarction. Which biochemical investigation out of
the followings would be of no help in the con rmation of diagnosis?
a)  Cardiac Troponins
b) Serum myoglobin
c) Lactate dehydrogenase
d) Creatine Phospho kinase-MB(CPK-MB)
21. A 56- year- old female presents with di culty opening her eyelids, as well as inability to
raise herself from sitting position. She is diagnosed with “myasthenia gravis”, a disease of
extreme fatigue, due to decreased concentration of Acetylcholine in her muscles. She has
been prescribed physostigmine, a drug that increases the amount of available Acetylcholine,
by competitively inhibiting acetylcholinesterase. Which of the following statements is not true
of competitive inhibitors?
A) Vmax remains the same
B) Apparent  Km in increased
C) Inhibitor is a structural analogue of the substrate
D) Inhibitor binds covalently to the enzyme
E) Increasing concentration of substrate can reverse the changes
22.Which of the following is a sulphur-containing amino acid that contains a disulphide bond (-
S-S-)?
A Serine
B Methionine
C Cysteine
D  Cystine
23. There is alfa-amylase, capable to cleave nutritive in saliva. What substrates of this
enzyme?
(A) Nucleoproteins
(B) Lipids
(C) Simple proteins
(D) Carbohydrates
(E) Chromo proteins
24 A 50 years old man experiences strong stress The concentration of adrenalin and
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24. A 50-years-old man experiences strong stress. The concentration of adrenalin and

Biochemistry
Noradrenalin was sharply increased in blood. What enzymes catalyze its inactivation process?
(A) Peptidase
(B) Glycosidase
(C) Monoamine oxidase
(D) Carboxylase
(E) Thyrosinase
25. Adult hemoglobin (HbA) – is a protein, which is formed by two alpha- and two beta-peptide
chains. What name has this structure of protein?
(A) Tertiary
(B) Quaternary
(C) Secondary
(D) Primary
26. Hem synthesis starts from glycine and succinyl-S-CoA interaction with help of enzyme
aminolevulinate synthetase. This enzyme activity is inhibited by the terminal metabolic
product – hem. Name the inhibition type:
(A) Feedback inhibition
(B) Uncompetitive regulation
(C) Non-competitive inhibition
(D) Limited proteolysis
(E) Competitive inhibition
27. Patient has diarrhea, dementia and dermatitis. Specify absence of what vitamin is the
cause of this state.
(A) Ascorbic acid
(B) Niacin
(C) Folic acid
(D) Biotin
(E) Rutin
28. Most participants of Magellan expedition to America died from avitaminosis. This disease
debuted with general weakness, subcutaneous hemorrhages, falling of teeth, gingival
hemorrhages. What is the name of this avitaminosis?
(A) Biermer's anemia
(B) Pellagra
(C) Rickets
(D) Polyneuritis (beriberi)
(E) Scurvy
29. For treatment of malignant tumors methotrexate (structural analogue of Folic acid) is
prescribed which is the competitive inhibitor of dehydrofolate reductase and consequently
suppresses synthesis of:
(A) Glycerophosphates
(B) Monosaccharides
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(B) Monosaccharides

Biochemistry
(C) Fatty acids
(D) Nucleotides
(E) Glycogen
30. According to clinical symptoms, pyridoxal phosphate was prescribed to the patient. For
correction of what processes this medicine is recommended?
(A) Deamination of purine nucleotides
(B) Oxidative decarboxylation of keto-acid
(C) Transamination and decarboxylation of amino acids
(D) Synthesis of purine and pyrimidine nucleotides
(E) Synthesis of proteins
31. A catalyst can promote product formation during a chemical reaction by _____.
(a) lowering the activation energy barrier.
(b) stabilizing the transition state.
(c) positioning reactants in the correct orientation.
(d) bringing reactants together.
(e) all of the above
32. Which of the following is characteristic of an enzyme catalyst?
(a) It positions reactants in the correct orientation.
(b) It lowers the activation energy barrier.
(c) It binds the transition state tighter than the substrate.
(d) all of the above
33. NAD+, FAD, and FMN are all cofactors for:
A. Oxidoreductases
B. Transferases
C. Hydrolases
D. Ligases
34. Invasive properties of pathogenic microorganisms are due to next enzymes
(A) Hydrolases
(B) Lyases
(C) Ligases
(D) Oxidoreductases
(E) Transferases
35. Marked increase of activity of MB-form of CPK (creatine phosphokinase) and LDH-1 were
revealed on the examination of the patient's blood. What is the most likely pathology?
A Cholecystitis
B Hepatitis
C Rheumatism
D Pancreatitis
E Miocardial infarction
36 The investigation of diseased person’s blood has revealed the excess increase of MB
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36. The investigation of diseased person s blood has revealed the excess increase of MB-
Biochemistry
isozyme of CPK (creatine phosphate kinase) and LDH1. Propose the probable pathology:
A. Myocardium infarction
B. Hepatitis
C. Rheumatism
D. Pancreatitis
E. Cholecystitis
37. The sick person was admitted to the hospital with previous diagnosis – acute pancreatitis.
Name enzyme, whose activity must be determined in the blood serum and urine to prove this
diagnosis:
A. Alpha-amylase
B. AlAT
C. AsAT
D. Lactate dehydrogenase
E. Choline esterase
38. Keloids were remained on patient’s body after burns. What enzyme-preparation may be
used for their resorption?
A. Asparaginase
B. Nigedase
C. Galactozidase
D. Streptolidase
E. Lidase
39. This enzyme is prescribed as preparation for treatment of purulent wounds. Name it:
A. Alkalic phosphatase
B. Amylase
C. Acidic phosphatase
D. Trypsin
E. Arginase
40. The patient with myocardium infarction was prescribed brinolytic drug “Streptodekase”
created using the linkage of enzyme with water- soluble polysaccharide template produced by
method:
A. Autolysis
B. Ultracentrifugation
C. Electrophoresis
D. Enzyme mobilization
E. Extraction
41. Which one of the following graphs shows the effect of pH on the reaction velocity of a
typical enzyme?

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Biochemistry

42. Consider the following Lineweaver-Burk plot for the following question. The solid line
indicates data observed in the absence of any inhibitor. The dotted lines labeled 1 and 2
indicate data observed in the presence two different inhibitors. Which of the following
represents the kinetic values of the enzyme in the presence of a non-competitive inhibitor?

     Km   Vmax
A) 0.5    5
B)  -2      0.4
C) 1        2.5
D) 0.5     2.5
43. Hem synthesis starts from glycine and succinyl-SCoA interaction with (aminolevulinate
synthetase help. It is inhibited by the terminal metabolic product – hem. Name the inhibition
type:
A. Feedback inhibition
B. Uncompetitive regulation
C. Non-competitive inhibition
D. Limited proteolysis
E. Competitive inhibition
44. Some pharmaceutical preparations containing ions of arsenic and mercury are used in
medical practice. Name type of inhibition of enzyme activity by these metal ions:
A. Reversible
B. Non-competitive
C. Uncompetitive
D. Competitive
E. Allosteric
45. Name factor of medium which can in uence the charge of functional groups in active site
of enzyme molecule:
A. Temperature of medium
B. Activator content in the medium
C.  pH of the medium
D. Pressure
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E. Allosteric inhibitor content in the medium

Biochemistry
I Connect organ and enzyme which can be used to detect disease in that organ
1.LDH1
A. Liver
2.Ala Transfarse
B. Skeletal muscles
3.Acid phosphatase
C. Heart
4.Regan Alac phosphatase
D. Cancer (ovary, uterus)
5.Aldose
E. Prostatic cancer

II Why is it said that Niacin and vitamin D are not true vitamins?

III A 56 – year-old male on Isoniazid therapy for tuberculosis has developed rashes on the
exposed parts of the body. What is the probable cause for these rashes?

1. One manifestation of vitamin A de ciency is

(A) Painful joints
(B) Night blindness
(C) Loss of hair
(D) Thickening of long bones
2. Scurvy is caused due to the de ciency of
(A) Vitamin A
(B) Vitamin D
(C) Vitamin K
(D) Vitamin C
3. Magenta tongue is found in the de ciency of the vitamin
(A) Ribo avin
(B) Thiamin
(C) Nicotinic acid
(D) Pyridoxine
4. The precursor of CoA is
(A) Ribo avin
(B) Pyridoxamine
(C) Thiamin
(D) Pantothenate
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(D) Pantothenate
Biochemistry
5. Which ion is a component of B12 core ring
(A) Cobalt
(B) Manganese
(C) Magnesium
(D) Iron
6. Loss of thiamin can be decreased by using
(A) Unpolished rice
(B) Parboiled rice
(C) Whole wheat our
(D) All of these
7. Pellagra preventing factor is
(A) Thiamin
(B) Ribo avin
(C) Niacin
(D) Pyridoxine
8. Pyridoxal phosphate is a coenzyme for
(A) Glutamate oxaloacetate transaminase
(B) Glutamate pyruvate transaminase
(C) Tyrosine transaminase
(D) All of these
9. Folic acid contains
(A) Pteridine
(B) p-Amino benzoic acid
(C) Glutamic acid
(D) All of these
10. Daily requirement of vitamin C in adults is about
(A) Potassium
 (B) Iodine
 (C) Iron
 (D) None of these
11. The symptoms of scurvy are
(A) Poor healing of wounds
(B) Loosening of teeth
(C) Anemia
(D) All of these
12. Symptoms of pellagra are
(A) Dermatitis and diarrhea only
(B) Dermatitis and dementia only
(C) Diarrhea, dermatitis and dementia
(D) Diarrhea and elements only
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(D) Diarrhea and elements only
Biochemistry
13. Antirickets vitamin is
(A) Vitamin A
(B)  Vitamin D
C) Vitamin E
(D)Vitamin K
14. After epileptic attack the baby was examined by the podiatrist. Baby was given arti cial
meal. Dermatitis was diagnosed. After laboratory tests the decrease of alanine and aspartate
aminotransferase level activity of erythrocytes was detected. What vitamin insu ciency can
be detected?
(A) Cobalamin
(B) Ascorbic acid
(C) Pyridoxine
(D) Ribo avin
(E) Calciferol
15. At the patient with frequent bleedings from an internal organs and mucous membrane,
proline and lysine as a part of collagen’s bers are found out. What vitamin absence causes
hydroxylation of proline and lysine?
(A) Thymine
(B) B1
(C) A
(D) C
(E) E
16. Hydroxyproline is the important amino acid in the structure of collagen. Which vitamin
does help in formation of this amino acid by hydroxylation of proline?
(A) B2
(B) D
(C) B1
(D) C
(E) B6
17. Any of the following processes except one are involved at the active site of an enzyme to
accelerate the rate of reaction-
a) Catalysis by Bond Strain
b) Catalysis by Proximity and Orientation
c) Non covalent catalysis
d) Acid base catalysis     
18. A 2-weeks –old child was brought to the emergency. The parents were fearful that the child
had been given some poison as they noted black discoloration on the diaper. A diagnosis of
Alkaptonuria was made and the child was given Vitamin C as a supplement. Alkaptonuria
occurs due to reduced activity of homogentisic acid oxidase enzyme. What is the role played
by vitamin C in this defect?
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by vitamin C in this defect?

Biochemistry
a) Acts as an oxidant
b)Acts as a coenzyme
c) Acts as an inducer
d) Acts as a positive allosteric modi er
19. Glycogen phosphorylase, which mobilizes glycogen for energy, requires which of the
followings as a cofactor?
a) Pyridoxal phosphate
b) Tetrahydrofolate
c) Adenosyl Cobalamin
d) Coenzyme A
20. A 42-year-old obese female presented to the emergency center with complaints of
worsening nausea, vomiting, and abdominal pain. Her pain was in the mid epigastric area and
right upper quadrant. Blood biochemistry revealed high serum amylase level. What is the
probable diagnosis for this patient?
a) Viral hepatitis
b) Acute Pancreatitis
c) Renal colic
d) Acute gastritis
21. Amino acid that contains a double ring in its structure: 
A Proline
B Tyrosine
C Tryptophan
D Phenylalanine
22. An allosteric inhibitor of an enzyme usually
(A) binds to the active site.
(B) participates in feedback regulation.
(C) denatures the enzyme.
(D) causes the enzyme to work faster.
(E) is a hydrophobic compound.
23. Aspirin owns a anti-in ammatory action, as inhibit cyclooxygenase activity. The level of
what bioactive molecules will go down?
(A) Prostaglandins
(B) Leukotrienes
(C) Catecholamines
(D) Biogenic amines
(E) Iodothyronines
24. Cation glycoproteins are the basic components of saliva of parotid glands. What amino
acids creates their positive charge?
(A) Aspartate, glutamate, glycine
(B) Lysine arginine histidine
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(B) Lysine, arginine, histidine

Biochemistry
(C) Aspartic acid, arginine, glutamic acid
(D) Glutamic acid, valine, leucine
(E) Cysteine, glycine, praline
25. Succinate dehydrogenase catalyzes the dehydrogenation of succinate. Malonic acid is
used to interrupt the action of this enzyme. Choose the inhibition type:
(A) Allosteric
(B) Competitive
(C) Non-competitive
(D) Dephosphorylation
(E) Limited proteolysis
26. During various diseases level of active forms of oxygen increases extremely that leads to
destruction of cellular membranes. For prevention of it antioxidants are used. Strong natural
antioxidant is:
(A) Glucose
(B) alpha-tocopherol
(C) Vitamin D
(D) Fatty acids
(E) Glycerin
27. People, who suffers from alcoholism, receive main part of calories with alcoholic
beverages. They can have a characteristic insu ciency of thiamine (a syndrome of Wernicke),
which causes a nervous system functions infringement, psychoses, memory loss. What
enzyme activity is decreased in this case?
(A) Transaminase
(B) Alcohol dehydrogenase
(C) Pyruvate dehydrogenase
(D) Aldolase
(E) Hexokinase
28. The oxidative decarboxylation of alpha-ketoglutarate is broken because of vitamin В1
de ciency. Synthesis of what coenzyme is broken?
(A) Nicotinamide adenine dinucleotide (NAD)
(B) Thiamine pyrophosphate (TPP)
(C) Flavin adenine dinucleotide (FAD)
(D) Lipoic acid (LA)
(E) Coenzyme A
29. Hydroxylation of endogenous substrates and xenobiotics requires a donor of protons.
Which of the following vitamins can play this role?
(A) Vitamin C
(B) Vitamin E
(C) Vitamin P
(D) Vitamin A
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(D) Vitamin A

Biochemistry
(E) Vitamin B6.
30. Which of the following statements concerning the effect of temperature change on an
enzyme-catalyzed reaction is correct?
A) An increase in temperature can stop the reaction by denaturing the enzyme.
B) An increase in temperature can increase the reaction rate by increasing the speed at which
molecules move.
C) An increase in temperature to the optimum temperature maximizes reaction rate.
D) more than one correct response
E) no correct response
31. An apoenzyme
A. Includes non-protein compounds such as metal ions
B. Consists of complex organic structures which may be classi ed as activation-transfer
coenzymes or oxidation-reduction coenzymes
C. Is the protein portion of the enzyme without the cofactors
D. None of the above
32. A competitive inhibitor of an enzyme works by
A. tting into the enzyme's active site
B. tting into the allosteric site of the enzyme
C. attaching itself to the substrate, thereby preventing the enzyme from contacting with
substrate
D. increasing the activation energy of the enzyme-catalyzed reaction
33. In a Lineweaver-Burk Plot, competitive inhibitor shows which of the following effect?
A. It moves the entire curve to right
B. It moves the entire curve to left
C. It changes the x-intercept
D. It has no effect on the slope
34. Protein kinases are enzymes that act on other enzymes by adding phosphates groups.
When the enzyme is phosphorylated, it changes its activity (it becomes activated or
deactivated depending on the enzyme). This regulatory mechanism of enzymatic activity is
called:
A) Allosteric Control
B) Competitive inhibition
C) Covalent Modi cation
D) Isozymes Modi cation
E) Zymogen activation
35. A patient presents high activity of LDH1, LDH2, aspartate aminotransferase, creatine
phosphokinase. In what organ (organs) is the development of a pathological process the most
probable?
A In the heart muscle (initial stage of myocardium infarction)
B In skeletal muscles (dystrophy atrophy)
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B In skeletal muscles (dystrophy, atrophy)

Biochemistry
C In kidneys and adrenals
D In connective tissue
E In liver and kidneys
36. It was revealed acute pancreatitis in patient using biochemical investigation of his blood
plasma. Find out biochemical test which can prove this diagnosis:
A. Acidic phosphatase activity
B. Amino transferases activity
C. Amylase activity
D. Creatinine content
E. Creatine phosphate kinase activity
37. Name enzymes whose activity is in need to determine in the blood plasma to diagnose and
to predict the development of disease for the patient with cardiac pathology:
A. PDH, MDH, KGDH
B. CPK (creatine phosphokinase), AlAT, AsAT
C. Arginase, peptidase, acidic phosphatase
D. Lysozyme, citrate synthase, aldolase
E. Neuraminidase, hexokinase, pyruvate kinase
38. The preparation named “Lidase” is used in clinics for resorption of keloids and hematomas
after burns and surgery operations. Name substrate for this enzyme preparation:
(A) Hyaluronic acid
(B) Dermatan sulfate
(C) Heparin
(D) Keratan sulfate
(E) Chondroitin-4-sulfate
39.Pathogenic microorganisms during their entering and reproduction in human tissues
produce different enzymes to help them in these actions. Find out those enzymes:
(A) Oxidase, Catalase
(B) Lipase, Ligase
(C) Transferase, nuclease
(D) Esterase, protease
(E) Hyaluronidase, Lecithinase
40. Five enzymes (LDH4, LDH5, AlAT, gamma-glutamyl transferase, carbamoyl ornithine
transferase) activities are increased in blood plasma of patient. Name organ (tissue) whose
damage is checked:
A. Liver
B. Heart
C. Lungs
D. Kidney
E. Pancreas
41 Asparaginase is used to reduce the level of asparagine in the blood in one treatment for
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41. Asparaginase is used to reduce the level of asparagine in the blood in one treatment for

Biochemistry
leukemia. Which isoform of asparaginase would be most useful if the patient’s blood
asparagine level is 0.2mM?
         Km       Vmax
A) 0.1mM; 0.5 mM/hr
B) 0.2mM; 0.1 mM/hr
C) 0.2mM; 0.5 mM/hr
D) 2.0mM; 0.1 mM/hr
E) 0.1mM; 0.1 mM/hr
42. Which of the diagrams illustrates the way in which the rate of an enzyme-controlled
reaction depends on substrate concentration?

43. The amino acid sequence of an enzyme was determined to have a genetic mutation
resulting in the amino acid change cysteine  proline that maps to the location on the structure
to the right highlighted by a box. The active site is designated by a circle which contains a zinc
cofactor shown as a black sphere and an inhibitor shown as white sticks. Of the following
options, what is the most likely effect of the mutation?

A) The mutant amino acid will directly interfere with catalysis.

B) The mutant amino acid is too far away to have any effect on the active site.
C) The mutant amino acid will alter interactions affecting the overall 3D enzyme structure.
D) The mutant amino acid will change the substrate speci city of the enzyme.
E) The enzyme will no longer bind its cofactor.
44. Heavy metal ions are very toxic. They block SH-groups that are placed in active centers of
enzymes. Name the type of enzyme inhibition for heavy metal ions:
A. Competitive
B. Allosterically
C. Non-competitive
D. Uncompetitive
E. Suicide
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45. The action of competitive inhibitor may be prevented by the:

Biochemistry
A. Increase of enzyme concentration
B. The use of metal ion in the medium
C. Increase of substrate concentration
D. The use of allosteric activator
E. The removal of the product from the medium

I. Combine enzyme and its class

1. Oxidoreductase
A. DNA-polymerase
2. Transferase
B. LDH
3. Hydrolase
C. Pepsin
4. Ligase
D. sGOT
5. Lyase
E. Aldolase

II. Name the vitamin that acts as a coenzyme for the post translational modi cations of
prothrombin.

III. A 5-year-old child with a previous fracture right foot had a serum Alkaline Phosphatase
(ALP) of 840 U/L (High),  a serum phosphate of 3.5 mg/dL (Normal),and a serum γ-glutamyl
transferase(GGT) of 30 U/L (Normal). There was no history of fever or any other medical
illness.
After the clotted blood was spun down in the test tube, the serum was visibly hemolyzed
owing to di culty in obtaining the specimen. The physical examination of the child was
unremarkable.
Why is the normal range of ALP higher in children than in adults?
What is the signi cance of a hemolyzed serum sample and the elevated serum ALP?
What is the most likely cause of the elevated ALP?

1. Both Wernicke’s disease and beriberi can be reversed by administrating

(A) Retinol
(B) Thiamin
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(B) Thiamin

Biochemistry
(C) Pyridoxine
(D) Vitamin B12
2. Increased glucose consumption increases the dietary requirement for
(A) Pyridoxine
(B) Niacin
(C) Biotin
(D) Thiamin
3. Pellagra is caused due to the de ciency of
 (A) Ascorbic acid
(B) Pantothenic acid
(C) Pyridoxine
 (D) Niacin
4. Pyridoxal phosphate is central to
(A) Deamination
(B) Aminations
(C) Carboxylation
(D) Transamination
5. Both folic acid and methyl cobalamin (vitamin B12) are required in
(A) Deamination of serine
(B) Deamination of threonine
(C) Conversion of pyridoxal phosphate to pyridoxamine phosphate
(D) Methylation of homocysteine to methionine
6. NADP is required as a coenzyme in
(A) Glycolysis
(B) Citric acid cycle
(C) HMP shunt
(D) Gluconeogenesis
7. A vitamin which can be synthesized by human beings is
(A) Thiamin
(B) Niacin
(C) Folic acid
 (D) Cyanocobalamin
8. Conversion of folate into tetrahydrofolate requires
(A) NADH
(B) NADPH
(C) FMNH2
(D) FADH2
9. Vitamin C activity is present in
(A) D-Ascorbic acid
(B) D Dehydroascorbic acid
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(B) D-Dehydroascorbic acid

Biochemistry
(C) L-Ascorbic acid
(D) Both A and B
10. The egg injury factor in raw egg white is
(A) Biotin
(B) Avidin
(C) Albumin
(D) Calcium salts
11. The following vitamin is involved in coenzyme function in transaminations:
(A) Nicotinamide
(B) Pyridoxine
(C) Thiamine
(D) Ribo avin
12. One of the main functions of Vitamin K is the cofactor for
(A) Carboxylase for the formation of γ-carboxyglutamate
(B) Methylation by S-adenosyl methionine
(C) Carboxylation by biotin
(D) One carbon transfer by tetrahydrofolate
13. Chose enzyme with absolute speci ty
A. Chymotrypsin
B. Hexokinase
C. Pepsin
D. Lipase
E. Diastase
14. Increased concentration of pyruvate is detected at the patient’s blood and urine. What
avitaminosis is observed at the patient?
(A) Avitaminosis B1
(B) Avitaminosis Е
(C) Avitaminosis В3
(D) Avitaminosis В6
(E) Avitaminosis В2
15. How non-competitive inhibitor in uence on curve position in Lineweaver -Burk plot
A. Move whole curve to write
B. Change y-cross position
C. Move whole curve to left
D. Change x-cross position
16. A given substrate may be acted upon by several different enzymes, each of which uses the
same substrate(s) and produces the same product(s). The individual members of a set of
enzymes sharing such characteristics are known as-
a) Group speci c enzymes
b) Isoenzymes
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b) Isoenzymes

Biochemistry
c) Substrate speci c enzymes
d) Allosteric enzymes
17. A 67- year-old army o cer in good health previously presented with sudden pain in the
great toe.  Serum uric acid level was high, and a diagnosis of gouty arthritis was made He was
advised bed rest, pain killers and Allopurinol. What is the mechanism of action of Allopurinol in
lowering serum uric acid levels?
a) Suicidal inhibition
b) Non-competitive inhibition
c) Allosteric inhibition
d) Feedback inhibition
18. Choose the incorrect statement about Active Site of an enzyme
a) The active site is a three-dimensional cleft
b) The active site takes up a large part of the total volume of an enzyme
c) Substrates are bound to enzymes by multiple weak attractions
d) The speci city of binding depends on the precisely de ned arrangement of atoms in an
active site.
19. A 60-year-old chronic alcoholic was brought to the hospital with complaints of protuberant
abdomen (ascites) and edema feet. He also had history of hemorrhages.  Blood biochemistry
revealed – High serum transaminases, low Serum total proteins, Albumin and a prolonged
prothrombin time. Urine analysis was normal. What could be the possible diagnosis?
a) Renal failure
b) Protein malnutrition
c) Cirrhosis of liver
d) Heart failure
20. Zinc is a prosthetic component of enzyme(s):
(A) carboxypeptidase
(B) alcohol dehydrogenase
(C) carbonic anhydrase
(D) succinate dehydrogenase
(E) All
21. Non-protein part of enzyme which participate in reaction and covalently bonded with
protein part is called
A. apoenzyme
B. prostetic group
C. proenzyme
D. cofactor
E. coenzyme
22. A non-competitive inhibitor of an enzyme catalyzed reaction
(A) binds to the Michaelis complex (ES).
(B) decreases Vmax
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(B) decreases Vmax.

Biochemistry
(C) is without effect at saturating substrate concentrations.
(D) can increase reaction velocity in rare cases.
(E) The rst and second choices are both correct.
23. At necropsy of a 40-year-old woman the legal expert found that death occurred because of
poisoning with cyanides. Blocking of which process is the most probable with the cyanides in
this state?
(A) Cytochrome oxidase
(B) Glycogen phosphorylase
(C) Pyruvate carboxylase
(D) Succinate dehydrogenase
(E) Glucose-6- phosphate dehydrogenase
24. Proteins presence in a solution can be found out by means of color reactions. Which one
of listed reactions proves the negative result during full proteins hydrolysis?
(A) Lead acetate test
(B) Ninhydrin reaction
(C) Xanthoprotein reaction
(D) Biuret reaction
(E) Sakaguchi reaction
25. What is the mode of action of the anticancer drug methotrexate?
a) It inhibits the absorption of folate from the intestine.
b) It inhibits dihydrofolate reductase so inhibiting thymidylate synthesis.
c) It inhibits the secretion of extrinsic factor which is needed for DNA synthesis.
d) It inhibits protein synthesis at the elongation stage.
26. At the young man 20 years old macrocytic anemia has emerged, increased level of methyl
malonic acid is registered in urine. It is caused by de ciency of:
(A) Pantothenic acid
(B) Nicotinic acid
(C) Cyanocobalamin
(D) Ascorbic acid
(E) Biotin
27. At the patient of 36 years old who suffers from chronic alcoholism, accumulation of
pyruvate is registered in blood, in erythrocytes – transketolase activity is decreased. What
coenzymes de ciency causes these changes?
(A) Pyridoxal phosphate
(B) Carboxybiotin
(C) Methyl cobalamin
(D) Thiamine pyrophosphate
(E) Tetrahydrofolate
28. Increased vessels, enamel and dentine destruction at scurvy patients are caused by
disorder of collagen maturing What stage of procollagen modi cation is damaged during this
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disorder of collagen maturing. What stage of procollagen modi cation is damaged during this

Biochemistry
avitaminosis?
(A) Hydroxylation of proline
(B) Formation of polypeptide chains
(C) Glycosylation of hydroxylysine residues
(D) Removal of C-ended peptide from procollagen
(E) Detaching of N-ended peptide.
29. What is the function of enzymes within living systems?
A) structural elements
B) neurotransmitters
C) catalysts
D) hormones
30. Enzymes have names that
A) always end in -ase
B) always end in -in
C) can end either in -in or -ase
D) can end in either -in or –ogen
31. An allosteric activator
A. increases the binding a nity
B. decreases the binding a nity
C. stabilizes the R state of the protein
D. both (a) and (c)
32. Allosteric enzymes are
A. similar to simple enzyme
B. smaller than simple enzyme
 C. larger and more complex than simple enzyme
 D. smaller than simple enzyme but not complex
33. Which ion activate salivary amylase
(A) Ca2+
(B) Cu2+
(C) Cl-
(D) Mg2+
34. Glucokinase catalyzes the transfer of phosphate group from ATP to glucose. This enzyme
is
(A) Lyases
(B) Oxidoreductase
(C) Isomerases
(D) Hydrolases
(E) Transferases
35. 6 hours after the myocardial infarction a patient was found to have elevated level of lactate
dehydrogenase in blood What isozyme should be expected in this case?
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dehydrogenase in blood. What isozyme should be expected in this case?
Biochemistry
A. LDH4
B. LDH1
C. LDH5
D. LDH3
E. LDH2
36. The patient of 47 y.o. with diagnosis myocardium infarction was admitted to reanimation
department of hospital. What isozyme of LDH (lactate dehydrogenase) will be elevated rst
two days in this patient’s blood plasma?
A. LDH1
B. LDH2
C. LDH 3
D. LDH 4
E. LDH 5
37. Name enzyme, whose activity determination in the blood serum is the most informative
test at rst hours of myocardium infarction development:
A. Creatine phosphate kinase
B. AsAT
C. AlAT
D. LDH
E. Glutamate dehydrogenase
38. The inhibitor for acetylcholine esterase was prescribed as preparation for the treatment of
patient. Choose it from the list:
(A) . Aspirin
(B) . Indometacin .
(C) Allopurinol
(D) Sodium dichlorfenak .
(E) Proserin
39. Treatment of patient with purulent wounds is made using dressing with enzyme
immobilized on them. Find out this enzyme:
A. Trypsin
B. Arginase
C. Catalase
D. Alkalic phosphatase
E. Acidic phosphatase
40. Trasilol is a preparation to treat acute pancreatitis, and it is found to block activity of
special pancreatic enzyme. Choose this enzyme:
A. trypsin
B. Amylase
C. Lipase
D Elastase
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D. Elastase
Biochemistry
E. Phospholipase A2
41. Refer to the Lineweaver-Burk plot below, showing the kinetic data representing ve similar
but distinct substrates of a single enzyme. Based on their kinetic parameters, which is the
most preferred substrate of the enzyme?

42. In the following diagram, each of the curves P, Q and R represents an enzyme-controlled
reaction in one of the following situations:
• with the enzyme uninhibited
• with a non-competitive inhibitor
• with a competitive inhibitor.
Which curve, P, Q or R, represents each situation?
uninhibited enzyme
competitive inhibitor added
non-competitive inhibitor added
43. Lipitor (a statin drug) inhibits the enzyme HMG-CoA Reductase in the pathway of
cholesterol biosynthesis via competitive inhibition. Normal kinetic parameters for this enzyme
with HMG-CoA (without the drug) are: Km = 8.1 µM and Vmax = 330 pmol min-Which of the
following shows the expected changes in the kinetic parameters for HMG-CoA in the presence
of the drug?
      Km                      Vmax
A) 8.1 µM          120 pmol min-1
B) 20.8 µM        120 pmol min-1
C) 20.8 µM      330 pmol min-1
D) 8.1 µM          330 pmol min-1
E) 2.3 µM           330 pmol min-1
44. Structure feature for regulatory enzyme usually is the presence of allosteric center in their
molecules. Find out its role:
A. It attaches the regulator-substance
B. It attaches the substrate
C. It changes the structure of substrate
D. It helps in dissociation of coenzyme
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E. It attaches the coenzyme

Biochemistry
45. Name a substance that competes with succinate to be attached to active site of Succinate
dehydrogenase:
A. Pyruvic acid
B. Amber acid
C. Malate
D. Lactate
E. alpha-Ketoglutarate

I. Combine enzyme and metal which is the component of this enzyme

1. Alcohol dehydrogenase
A. Fe2+
2. Cytochrome oxidase
B. K+
3. Pyruvate kinase
C. Mg2+
4. Xanthine oxidase
D. Zn2+
5. Hexokinase
E. Mo4+

II The synthesis of which vitamin is inhibited by Sulphonamides?

III. A 44-year-old man who had lost his job because of absenteeism, presented to his physician
complaining of loss of appetite, fatigue, muscle weakness, and emotional depression.
The physical examination revealed a somewhat enlarged liver that was rm and nodular, and
there was a hint of jaundice in the sclerae and a hint of alcohol in his breath. The initial
laboratory pro le included a hematological analysis that showed that he had an anemia with
enlarged red blood cells (macrocytic). A bone marrow aspirate con rmed the suspicion that he
had a megaloblastic anemia because it showed a greater than normal number of red and
white blood cell precursors, most of which were larger than normal. Further analyses revealed
that his serum folic acid level was1.2 ng/mL (normal 2.5 to20), his serum B12 level was 253
ng/mL (normal 200 to 900), but his serum iron level was normal.
What is the cause of megaloblastic anemia in this patient?

1. De ciency of vitamin D causes

(A) Rickets and osteomalacia
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(A) Rickets and osteomalacia
Biochemistry
(B) Tuberculosis of bone
(C) Hypothyroidism
(D) Skin cancer
2. The Vitamin B1 de ciency causes
(A) Rickets
(B) Nyctalopia
(C) Beriberi
(D) Pellagra
3. Niacin is synthesized in the body from
(A) Tryptophan
(B) Tyrosine
(C) Glutamate
(D) Aspartate
4. Biotin is a coenzyme of the enzyme
(A) Carboxylase
(B) Hydroxylase
(C) Decarboxylase
(D) Deaminase
5. Folate as a coenzyme is involved in the transfer and utilization of
(A) Amino group
(B) Hydroxyl group
(C) Single carbon moiety
(D) Amino group
6. Thiamin requirement is greater in
(A) Non-vegetarians
(B) Alcoholics
(C) Pregnant women
(D) Both B and C
7. Which ion is necessary for activity of carbonic anhydrase
A. Cl
B. Zn
C. Mg
D. Mn
E. Fe
8. Ribo avin de ciency symptoms are
(A) Glossitis
(B) stomatitis
(C) Vomiting
(D) Both (A) and (B)
9 De ciency of vitamin C causes
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9. De ciency of vitamin C causes
Biochemistry
(A) Beriberi
(B) Pellagra
(C) Pernicious anemia
(D) Scurvy
10. The human species can biosynthesize
(A) Vitamin C
(B) Vitamin B12
(C) Thiamine
(D) Niacin
11. De ciency of Vitamin A leads to
(A) Rickets
(B) Scurvy
(C) Night blindness
(D) All of these
12. This Vitamin acts as antioxidant:
(A) Vitamin A
(B) Vitamin D
(C) Vitamin E
(D) Vitamin K
13. Treatment of some infectious diseases which are caused by bacteria is based on,
sulfanilamide medicines. These medicines block synthesis of the growth factor of bacteria.
Choose the mechanism of sulfanilamide’s medicines:
(A) Inhibition of absorption of folic acid
(B) these drugs are antivitamins of paraaminobenzoic acid inhibitor of same enzymes
(C) these drugs take part in oxide-redactions reactions
(D) these drugs are allosteric enzymes of paraaminobenzoic acid
14. Anticoagulant pelentan was prescribed to the patient with thromboembolia. What vitamin
antagonist is this compound?
(A) Vitamin C
(B) Vitamin E
(C) Vitamin A
(D) Vitamin D
(E) Vitamin K
15. Patient suffers from diarrhea, dementia and dermatitis. At the anamnesis is known that the
basic foodstuff of the patient is corn. These infringements relate to malnutrition of vitamin:
(A) Vitamin В1
(B) Vitamin pp
(C) vitamin В2
(D) vitamin В9
(E) vitamin В8
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(E) vitamin В8
Biochemistry
16. One out of the following enzymes has absolute speci city for its substrate; choose the
correct option
a) Urease
b) Carboxypeptidase
c) Pancreatic lipase
d) Lipoprotein lipase
17. A competitive inhibitor of an enzyme is usually:
(A) a highly reactive compound.
(B) a metal ion such as Hg2+ or Pb2+.
(C) structurally like the substrate.
(D) water insoluble.
(E) a poison
18. In adjustment of enzymes activity an important place belongs to their post synthetic
covalent modi cation. What from the noted mechanisms is used to control glycogen
phosphorylase and glycogen synthase activity?
(A) Methylation
(B) Phosphorylation-dephosphorylation
(C) Adenyle addition
(D) Limited proteolysis
(E) ADP-ribosylation
19. The biological oxidation and deactivation (detoxication) of xenobiotics are realized by
hem-containing enzymes. Which metal is a required component of these enzymes?
(A) Mg
(B) Zn
(C) Co
(D) Fe
(E) Mn
20. Aspirin has anti-in ammatory and analgesic action, as inhibit the biosynthesis of
prostaglandins. What enzyme is inhibited by aspirin?
(A) Decarboxylase
(B) Dehydrogenase
(C) Hydroxylase
(D) Cyclooxygenase
(E) Deaminase
21. Proteins presence in a solution can be found out by means of color reactions. Which one
of listed reactions proves the negative result during full proteins hydrolysis?
(A) Lead acetate test
(B) Ninhydrin reaction
(C) Xanthoprotein reaction
(D) Biuret reaction
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(D) Biuret reaction
Biochemistry
(E) Sakaguchi reaction
22. Treatment of some infectious diseases which are caused by bacteria is based on,
sulfanilamide medicines. These medicines block synthesis of the growth factor of bacteria.
Choose the mechanism of sulfanilamide’s medicines:
(A) Inhibition of absorption of folic acid
(B) these drugs are antivitamins of paraaminobenzoic acid
(C) inhibitor of same enzymes
(D) these drugs take part in oxide-redactions reactions
(E) these drugs are allosteric enzymes of paraaminobenzoic acid
23. At patient, with frequent bleedings in to visceral organs and mucous membranes, in the
structure of collagen bers proline and lysine were found out. Absence of what vitamin causes
such infringement of hydroxylation of this amino acid?
(A) Vitamin E
(B) Vitamin C
(C) Vitamin K
(D) Vitamin A
(E) Vitamin D
24. It is known that the collagen molecule contains amino acids (oxyproline, oxylysine). Which
one of the listed low substances takes part in hydroxylation of proline and lysine during
synthesis?
(A) Asparagine acid
(B) Folic acid
(C) Pantothenic acid
(D) Glutamic acid
(E) Ascorbic acid
25. During parodontosis treatment an antioxidant of a natural and arti cial origin is used.
Specify, what of natural compounds is used as antioxidant?
(A) Gluconate
(B) Thiamine
(C) Tocopherol
(D) Pyridoxine
(E) Choline
26. If there is insu ciency of thiamine - vitamin В1, bery-bery disease (polyneuritis) and
carbohydrate metabolism disorder occur. What metabolite accumulates in blood during these
processes?
(A) Malate
(B) Lactate
(C) Succinate Citrate
(D) Pyruvate
27 There is the osteoporosis in the patient with chronic renal failure What vitamin’s
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27. There is the osteoporosis in the patient with chronic renal failure. What vitamin s
Biochemistry
metabolism infringement promotes this disorder?
(A) Vitamin D
(B) Vitamin E
(C) Vitamin A
(D) Vitamin K
(E) Vitamin C
28. Which of the following statements about enzymes or their function is true?
A. Enzymes do not alter the overall change in free energy for a reaction
B. Enzymes are proteins whose three-dimensional form is key to their function
C. Enzymes speed up reactions by lowering activation energy
D. All the above
29. Which of the following statements about allosteric enzymes is CORRECT?
A. The binding of substrate to any active site affects the other active sites
B. The plot of initial velocity vs. substrate concentration is a straight line
C. The Keq of the reaction is increased when allosteric activator is bound
D. The enzymes contain only one polypeptide chain
30. An uncompetitive inhibitor binds to _____.
(a) E
(b) ES
(c) P
(d) a and b
(e) a and c
31. Allosteric modulators seldom resemble the substrate or product of the enzyme. What does
this observation show?
 A) Modulators likely bind at a site other than the active site.
B) Modulators always act as activators.
 C) Modulators bind non-covalently to the enzyme.
 D) The enzyme catalyzes more than one reaction.
32. Which statement is false about allosteric regulation?
A. It is usually the mode of regulation for the last step in reaction pathways since this step
produces the nal product.
B. Cellular response is faster with allosteric control than by controlling enzyme concentration
in the cell.
C. The regulation usually is important to the conservation of energy and materials in cells.
D. Allosteric modulators bind non-covalently at sites other than the active site and induce
conformational changes in the enzyme.
33. Pyridoxal phosphate is required as a coenzyme in
(A) Transamination
(B) Transsulphuration
(C) Desulphyration
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(C) Desulphyration
Biochemistry
(D) All of these
34. Enzymes which are main to promote biological oxidation
(F) Lyases
(G) Oxidoreductase
(H) Isomerases
(I) Hydrolases
(J) Transferases
35. There is medicine named Teturam used in medical practice for the treatment and
prophylaxis of alcoholism, and this preparation is inhibitor of aldehyde dehydrogenase.
Accumulation of what compound in the blood of alcoholics will cause the aversion to alcohol
under the use of this preparation?
A. Acetic aldehyde
B. Ethanol
C. Malonic dialdehyde
D. Propionic aldehyde
E. Methanol
36. The woman of 50 y.o. with diagnosis myocardium infarction was admitted in the
department of intensive therapy. Name the enzyme whose activity will be elevated two days in
the blood plasma of this diseased person:
A. Alanine aminotransferase
B. Alanine peptidase
C. Aspartate aminotransferase
D. LDH4
E. LDH5
37. The patient has acute pancreatitis. Name preparation that will be recommended by doctor
for the treatment to prevent autolysis of pancreas:
A. Proteases inhibitors
B. Proteases activators
C. Trypsin
D. Chymotrypsin
E. Amylase
38. 46 years old patient’s blood serum creatine phosphate kinase activity was determined, and
it was increased in values. Name the pathology that may be discussed for this patient:
(A) Chronic hepatitis
(B) Myocardium infarction .
(C) Hemolytic anemia
(D) Acute pancreatitis .
(E) Renal insu ciency
39. The most informative test for early diagnosis of muscular dystrophies is the increase in
the blood plasma of enzyme activity named:
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the blood plasma of enzyme activity named:
Biochemistry
A. AsAT
B. AlAT
C. CPK-3
D. LDH1
E. Hexokinase
40. The degree of liver parenchyma damage may be estimated due to determination in the
blood plasma of:
A. Choline esterase activity
B. Amylase activity
C. LDH3 content
D. Acidic phosphatase activity
E. LDH1 and LDH2 content
41. Which antibiotic blocks the active site of an enzyme that many bacteria used to make cell-
walls.
(A) Amphotericin
(B) Gentamicin
(C) Penicillin
(D) Cephalosporin

42. The graph shows an enzyme-controlled reaction. The enzyme concentration is kept
constant.
At concentrations of substrate greater than X, which of the following statements is true?
A The rate of reaction is limited by enzyme concentration.
B The rate of reaction tends towards zero.
C The substrate has an inhibitory effect.
D The products have an inhibitory effect.
Curve X represents the relationship between an enzyme and the concentration of its substrate
under optimal conditions and without an inhibitor.

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Biochemistry
43. Which one of the curves A, B, C or D represents the result when the same experiment is
conducted in the presence of a xed, low concentration of a reversible, competitive inhibitor?
44. Which one of the curves A, B, C or D represents the result when the same experiment is
conducted in the presence of a xed, low concentration of an irreversible, non-competitive
inhibitor?
45. Acetyl-CoA-carboxylase (the key enzyme in fatty acid synthesis) is regulated by feed-back
in uence of end-product – Palmityl-CoA. Feed-back inhibition is subtype of:
A. Allosteric inhibition
B. Irreversible inhibition
C. Competitive inhibition
D. Covalent modi cation
E. Uncompetitive inhibition

I. Combine drug and disease where it used

1. Allopurinol
A. Muscle relaxant
2. Asparginase
B. Leukemia
3. Sulfonilamide
C. Gout
4. Succinilcholine
D. Infection disease
5. Neostigmine
E. Myastegnia gravis

II. Which vitamin de ciency cause prolonged administration of izoniazide (used for
tuberculosis treatment)

III. A  4 -year-old boy was brought for consultation for hematuria, edema of lower extremities
as well as swollen right leg. He was the 12the born in a poor family, where one previous child
died from malnutrition and dehydration in the period of infancy.  The child was fed only with
cow’s milk and biscuits.
From the period of ve months, the boy manifested irritability, sweating, poor appetite and
cried when somebody touched him
At admission the baby was afebrile, pale, and malnourished; his hair was dry and cracked.
Clinical evaluation showed no organomegaly, no neurological signs, gingival bleeding was
there and only one tooth was present.
Laboratory ndings were as follows: Red Blood Cell Count 3.5 million/mm3; Hemoglobin (Hb)
7 g/dl; Haemtocrit (Hct) 30%; Serum Iron low
Liver functional tests were in the normal range Ultrasound of kidney was normal Doppler of
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Liver functional tests were in the normal range. Ultrasound of kidney was normal. Doppler of
Biochemistry
blood vessels of both legs was normal which excluded thrombophlebitis. Swelling of the right
leg indicated radiological investigation. Massive subperiosteal hematoma on the right femur,
dilatated metaphyses and general osteoporosis had been present on the radiogram.
What is the probable diagnosis for this child?

1. Vitamin E reduces the requirement of

(A) Iron
(B) Zinc
(C) Selenium
(D) Magnesium
2. Vitamin B1 coenzyme (TPP) is involved in
(A) Oxidative decarboxylation
(B) Hydroxylation
(C) Transamination
(D) Carboxylation
3. In the body 1 mg of niacin can be produced from
(A) 60 mg of pyridoxine
(B) 60 mg of tryptophan
(C) 30 mg of tryptophan
(D) 30 mg of pantothenic acid
4. The vitamin required as coenzyme for the action of transaminases is
(A) Niacin
(B) Pantothenic acid
(C) Pyridoxal phosphate
(D) Ribo avin
5. A de ciency of vitamin B12 causes
(A) Beri-Beri
(B) Scurvy
(C) Pernicious anemia
(D) Rickets
6. People consuming polished rice as their staple food are prone to
(A) Beriberi
(B) Pellagra
(C) Both (A) and (B)
(D) None of these
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(D) None of these
Biochemistry
7. A classical uncompetitive inhibitor is a compound that binds
A. reversibly to the enzyme substrate complex yielding an inactive ESI complex
B. irreversibly to the enzyme substrate complex yielding an inactive ESI complex
C. reversibly to the enzyme substrate complex yielding an active ESI complex
D. irreversibly to the enzyme substrate complex yielding an active ESI complex
8. Anti-egg white injury factor is
(A) Pyridoxine
(B) Biotin
(C) Thiamin
(D) Liponic acid
9. Vitamin B12 forms coenzymes known as
(A) Cobamine
(B) Transcobalamine I
(C) Transcobalamine II
(D) Both (B) and (C)
10. The vitamin having the highest daily requirement among the following is
(A) Thiamin
(B) Ribo avin
(C) Pyridoxine
(D) Ascorbic acid
11. A de ciency of folate leads to
(A) Megaloblastic anemia
(B) Aplastic anemia
(C) Pernicious anemia
(D) Hypochromic microcytic anemia
12. Antisterility vitamin is
(A) Vitamin B1
(B) Vitamin B2
(C) Vitamin E
(D) Vitamin K
13. Chose photolabile vitamin:
(A) Thiamine
(B) Ribo avin
(C) Niacin
(D) Cholecalciferol
14. On reception to the doctor the patient with symmetric dermatitis of open sites of skin has
come. From patient’s story it is established that he eats, basically, cereals and few meat, milk
and eggs. De ciency of which vitamin is principally detected?
(A) Folic acid
(B) Calciferol
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(B) Calciferol
Biochemistry
(C) Nicotinic acid
(D) Biotin
(E) Tocopherol
15. The patient complains on general weakness and bleeding from gums. What vitamin lack
can cause such state?
(A) Vitamin A
(B) Vitamin E
(C) Vitamin C
(D) Vitamin H
(E) Vitamin D
16. The oculist has found out increase of adaptation time of an eye to the darkness at patient.
What vitamin insu ciency can cause such symptoms?
(A) Vitamin C
(B) Vitamin E
(C) Vitamin A
(D) Vitamin K
(E) Vitamin D
17. Which statement out of the following is incorrect about the effect of increasing
temperature on enzyme activity-
a) Raising the temperature increases the kinetic energy of molecules
b) A ten degree C rise in temperature will increase the activity of most enzymes by 50 to 100%.
c) Most animal enzymes rapidly become denatured at temperatures above 40oC
d) Storage of enzymes at 5°C or below is generally not suitable.
18. Group I enzymes participate in following reactions-
a) Oxidation-reduction
b) Transamination
c) Phosphorylation
d) All of the above
19. A given substrate may be acted upon by a number of different enzymes, each of which
uses the same substrate(s) and produces the same product(s). The individual members of a
set of enzymes sharing such characteristics are known as-
a) Group speci c enzymes
b) Isoenzymes
c) Substrate speci c enzymes
d) Allosteric enzymes
20. A 67- year-old army o cer in good health previously presented with sudden pain in the
great toe.  Serum uric acid level was high, and a diagnosis of gouty arthritis was made He was
advised bed rest, pain killers and Allopurinol. What is the mechanism of action of Allopurinol in
lowering serum uric acid levels?
a) Suicidal inhibition
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a) Suicidal inhibition
Biochemistry
b) Non competitive inhibition
c) Allosteric inhibition
d) Feed back inhibition
21. Chose a basic amino acid:
A Glutamate
B hydroxyproline
C citrulline
D selenocysteine
22. Lipoic acid was excluded from diet of the experimental animals, and during this process
the inhibition of their pyruvate dehydrogenase complex was observed. What is lipoic acid for
this enzyme?
(A) Allosteric regulator
(B) Substrate
(C) Inhibitor
(D) Coenzyme
(E) Product
23. Potassium cyanide is a poison death of the body comes instantly. Name of the enzyme in
mitochondria on which cyanic potassium can in uence (affect) is:
(A) Cytochrome b5
(B) Flavin-containing enzymes
(C) Cytochrome oxidase
(D) NAD - dependent dehydrogenase
(E) Cytochrome P-450
24. The prolonged use of large doses of aspirin causes suppression of prostaglandins
synthesis. Which enzyme has reduced activity?
(A) Cyclooxygenase
(B) Peroxidase
(C) 5-lipoxygenase
(D) Phospholipase-A2
(E) Phosphodiesterase
25. Research of electrophoretic spectrum of albumens of blood serum was carried out to a
patient with hepatic failure. What physical and chemical properties of albumins molecules are
in the basis of this method?
(A) Presence of charge
(B) Hydrophilic properties
(C) Ability to swell up
(D) Optical activity
26. Sulphanilamide drugs are similar by their structure to paraaminobenzoic acid. What is the
base of their pharmacological effect?
(A) activating of lipolysis
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(A) activating of lipolysis
Biochemistry
(B) binding with DNA
(C) inhibition of glycolysis
(D) infringement of vitamin synthesis
(E) destruction of cellular membrane
27. The megaloblastic anemia is diagnosed at the patient. Specify the substance which
insu ciency can cause the development of this illness.
(A) Cholecalciferol
(B) Glycine
(C) Copper
(D) Cyanocobalamine
(E) Magnesium
28. While examining the child, doctor found symmetric cheeks roughness, diarrhea and
dysfunction of the nervous system. Lack of what food components caused it?
(A) Threonine, pantothenic acid
(B) Methionine, lipoic acid
(C) Lysine, ascorbic acid
(D) Nicotinic acid, tryptophan
(E) Phenylalanine, pangamic acid
29. Malignant macrocytic anemia – Birmer disease– is a result of vitamin В12 lack. What
microelement is a part of this vitamin?
(A) Zinc
(B) Molybdenum
(C) Cobalt
(D) Iron
(E) Magnesium
30. Hypovitaminosis of vitamin C leads to reduction of organic matrix formation and
infringement of collagen synthesis because this vitamin participates in processes of:
(A) Proline carboxylation
(B) Proline hydroxylation
(C) Lysine carboxylation
(D) Arginine hydroxylation
(E) Tryptophan hydroxylation.
31. Name the enzyme class, whose structure often contains vitamin PP (its derivatives NAD or
NADP):
(A) Oxidoreductases
(B) Hydrolases
(C) Ligases
(D) Isomerases
(E) Lyases
32 An enzyme active site is the location in an enzyme where substrate molecules
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32. An enzyme active site is the location in an enzyme where substrate molecules
Biochemistry
A) are generated.
B) become catalysts.
C) undergo change.
D) more than one correct response
E) no correct response
33. At the end of a chemical reaction
A. an enzyme's structure is altered
B. an enzyme is detached from the product, has its original structure, and can catalyze more
chemical reactions
C. the enzyme loses its ability to catalyze other chemical reactions
D. the enzyme remains attached to the products
34. Conversion of proline into hydroproline and lysine into hydroxyl-lysine in collagen
molecules catalyzed by
(K) Lyases
(L) Oxidoreductase
(M) Isomerases
(N) Hydrolases
(O) Transferases
35. A sick woman of 46 y.o. is suffered from progressive muscular dystrophy (Duchenne
dystrophy). Name enzyme whose activity in the blood plasma is diagnostic test to prove this
disorder:
A. Creatine phosphokinase
B. Lactate dehydrogenase
C. Pyruvate dehydrogenase
D. Glutamate dehydrogenase
E. Adenylate kinase
36. The young man of 18 y.o. with the damage of parenchyma of the liver has in his blood
plasma probable elevated level of this enzyme activity:
A. Alanine aminotransferase
B. LDH1
C. Creatine kinase
D. Acidic phosphatase
E. Alpha-amylase
37. Hemorrhagic stroke is observed in patient. There is increase of kinins level in the blood of
patient. Contrical preparation was prescribed for the patient to treat him. Name protease
whose activity will be inhibited by this preparation in patient’s blood:
A. Kallikrein
B. Pepsin
C. Trypsin
D Chymotrypsin
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D. Chymotrypsin
Biochemistry
E. Collagenase
38. Pathogenic microorganism contain aggression enzymes, that promote virulence for
microorganisms. Find out those one:
(A) . Lyase
(B) . Carbo anhydrase .
(C) Hyaluronidase
(D) Oxidase
(E) Transferase
39. New antibiotics are produced from natural ones due to enzymatic reactions. Name
synthetic form of enzymes frequently used in pharmaceutical industry:
A. Immobilized enzyme
B. Natural enzyme
C. Denaturated enzyme
D. Enzyme complex with coenzyme
E. Enzyme complex with activator
40. Find out the enzyme whose activity is determined in the blood plasma of patient with
bone tissue pathology:
A. Pepsin
B. Trypsin
C. Amylase
D. Acidic phosphatase
E. Alkalic phosphatase
41. A substrate S can be degraded by two different isozymes (#1 and #2), both obeying
Michaelis-Menten kinetics.  The Km for isozyme #1 is 0.1 mM and the corresponding value for
isozyme #2 is 10 mM.  Both isozymes have the same Vmax.  If [S] = 5 mM, which of the
following statements is correct?
A) Isozyme #1 will degrade S faster than isozyme #2.
B) Isozyme #2 will degrade S faster than isozyme #1.
C) Isozyme #1 will degrade S at a rate equal to half of its maximal velocity.
D) Isozyme #2 will degrade S at a rate equal to half of its maximal velocity.
E) Isozyme #2 will degrade S at its maximal velocity.
42.  Penicillinase is an enzyme present in some bacteria that can inactivate the antibiotic
penicillin. Based on the Lineweaver Burk plot below, determine the Vmax and Km of
penicillinase.

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Biochemistry

43. Proserin (neostigmine) was used for the treatment of myasthenia and other disorders of
muscular tissue. This preparation is competitive inhibitor of enzyme:
A. Lactate dehydrogenase
B. Citrate synthase
C. Succinate dehydrogenase
D. Arginase
E. Acetylcholine esterase
44. Phosphor-containing organic compounds (mainly, they are poisons to cause CNS
paralysis) inhibit acetyl choline transferase across blockage of OH-groups of serine residues in
active sites of enzymes. Name type of inhibition for these compounds:
A. Irreversible
B. Reversible
C. Competitive
D. Non-competitive
E. Feed-back inhibition
45. Some insecticides are poisons for humans because they block irreversibly the activity of
very important enzyme in nervous tissue. It is:
A. Cytochrome C oxidase
B. ATP synthase
C. Acetylcholine esterase
D. Pyruvate kinase
E. Lactate dehydrogenase

I. Match drug and enzyme which inhibited by this drug

1. Methotrexate
A. Cytochrome oxidase
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2. Allopurinol
Biochemistry
B. Acetylcholine esterase

3. Cyanide
C. Dihydrofolate reductase

4. Amphetamine
D. Xanthine oxidase

5. Diisopropyl uorophosphates
E. MAO

II. A homeless person has been brought to emergency. Blood biochemistry reveals high lactate
levels. An impaired activity of PDH complex is suspected. Which vitamins de ciency can
cause such symptoms?

III. Man suffers from short breathing. He smokes 1 pack cigarettes a day. Genetic screening
revealed that his has variety of AT-SS. What his diagnose and what medicine should be
prescribed?

1. The pellagra preventive factor is

(A) Ribo avin
(B) Pantothenic acid
(C) Niacin
(D) Pyridoxine
2. The enzymes with which nicotinamide act as coenzyme are
(A) Dehydrogenases
(B) Transaminases
(C) Decarboxylases
(D) Carboxylases
3. The coenzyme required for conversion of pyruvate to oxaloacetate is
(A) FAD
(B) NAD
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(B) NAD
Biochemistry
(C) TPP
(D) Biotin
4. Sulpha drugs interfere with bacterial synthesis of
(A) Lipoate
(C) Tetrahydrofolate
(B) Vitamin E
(D) Ascorbic acid
5. Niacin can be synthesized in human beings from
(A) Histidine
(B) Phenylalanine
(C) Tyrosine
 (D)Tryptophan
6. When eggs are cooked
(A) Biotin is destroyed but avidin remains unaffected
(B) Avidin is inactivated but biotin remains unaffected
(C) Both avidin and biotin are inactivated
(D) Both avidin and biotin remain unaffected
7. Methylcobalamin is required for formation of
(A) Serine from glycine
(B) Glycine from serine
(C) Methionine from homocysteine
(D) All of these
8. This vitamin acts as antioxidant.
(A) Vitamin A
(B) Vitamin D
(C) Vitamin E
(D) Vitamin K
9. Biotin is essential for
(A) Translation
(B) Carboxylation
(C) Hydroxylation
(D) Transamination
10. Carrot, pumpkin and other red vegetables contain carotin. De ciency of what vitamin is
supplied with these vegetative pigments?
(A) Tocopherol
(B) Naftohinon
(C) Ribo avin
(D) Retinol
(E) Calciferol
11 During child (age of 11 months) examination by pediatrist bended bones of the low limbs
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11. During child (age of 11 months) examination by pediatrist, bended bones of the low limbs
Biochemistry
and low bones mineralization of skull were found. What vitamin lack leads to the pathology?
(A) Thiamin
(B) Cholecalciferol
(C) Pantothenic acid
(D) Bio avonoid
(E) Ribo avin
12. The patient complained on the general weakness and bleeding from gums. What vitamin
insu ciency can it be at patient?
(A) Vitamin В1
(B) Vitamin E
(C) Vitamin РР
(D) Vitamin D
(E) Vitamin C
13. 42-year-old obese female presented to the emergency center with complaints of worsening
nausea, vomiting, and abdominal pain. Her pain was in the mid epigastric area and right upper
quadrant. Blood biochemistry revealed high serum amylase level. What is the probable
diagnosis for this patient?
a) Viral hepatitis
b) Acute Pancreatitis
c) Renal colic
d) Acute gastritis
14. The drug Fluorouracil is recommended for the treatment of cancers. It undergoes a series
of changes and then binds to Thymidylate synthase enzyme resulting in its inhibition and
blockage of cell division. This mode of inhibition is most probably due to-
a) Allosteric inhibition
b) Competitive inhibition
c) Noncompetitive Inhibition
d) Suicidal inhibition                   
15. Which statement out of the followings is incorrect about the effect of increasing
temperature on enzyme activity-
a) Raising the temperature increases the kinetic energy of molecules
b) A ten degree C rise in temperature will increase the activity of most enzymes by 50 to 100%.
c) Most animal enzymes rapidly become denatured at temperatures above 40oC
d) Storage of enzymes at 5°C or below is generally not suitable.
16. The degree of inhibition α by a competitive inhibitor is obtained from
(A) measurement of Vmax.
(B) measurement of the y-intercept on a Limeweaver-Burke Plot.
(C) measurement of Km.
(D) crystallographic studies.
(E) is unrelated to the binding a nity of the inhibitor to the enzyme
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8/4/2018 MCQ module 1
(E) is unrelated to the binding a nity of the inhibitor to the enzyme.

Biochemistry
17. During the pathological processes, accompanied by hypoxia, incomplete
reduction of oxygen molecules in respiratory chain and the accumulation of hydrogen peroxide
is take place. Specify the enzyme, which catalyze its destruction.
(A) Aconitase
(B) Cytochrome oxidase
(C) Peroxidase
(D) Ketoglutarate dehydrogenase
(E) Catalase
18. Prolonged use of large doses of aspirin causes the inhibition of synthesis of
prostaglandins by reducing the activity of the enzyme:
(A) Peroxidase
(B) Cyclooxygenase
(C) 5- lipoxygenase
(D) Phospholipase A-2
(E) Phosphodiesterase
19. Different functional groups can be presented in the structure of L-amino acid’s radicals.
Identify the group that can form ester bond:
(A) –OH
(B) -CONH2
(C) -SH
(D) -NH2
(E) -CH3
20. Which of the following statements about uric acid is correct?
a) Uric acid is readily soluble in the blood.
b) Overproduction of PRPP leads to the formation of excess uric acid.
c) Uric acid is the oxidized product of purines and pyrimidines
d) Allopurinol is a suicide inhibitor of PRPP aminotransferase.
21. Dermatitis has appeared at patient after eating raw (unboiled) eggs. What avitaminosis
has emerged?
(A) Avitaminosis of folic acid
(B) Avitaminosis of biotin
(C) Avitaminosis of tocoferol
(D) Avitaminosis of thiamin
22. The gerontology institute advises to old people to use a complex of vitamins which
contains vitamin E. What main function does it carry out?
(A) Antiscorbutic function
(B) Antihemorrhagic function
(C) Antioxidant function
(D) Antineuritic function
(E) Antidermatitic function
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8/4/2018 MCQ module 1
(E) Antidermatitic function

Biochemistry
23. For diagnostics of some diseases, aminotransferase activity is researched in blood. What
vitamin is a coenzyme of these enzymes?
(A) b6
(B) В2
(C) b1
(D) В9
(E) В5
24. Concentration of pyruvate is increased in the patient's blood, the most of which is excreted
with urine. What avitaminosis is observed in the patient?
(A) Avitaminosis b1
(B) Avitaminosis E
(C) Avitaminosis В3
(D) Avitaminosis B6
(E) Avitaminosis В2
25. Sample questions The protein portion of a conjugated enzyme is called a(n)
 A) apoenzyme.
B) coenzyme.
 C) holoenzyme.
 D) cofactor.
26. Which of the following could be a component of a conjugated enzyme?
A) coenzyme
B) cofactor
C) apoenzyme
D) more than one correct answer
E) no correct response
27. The location on an enzyme where binding occurs is known as the
A. action point
 B. enzyme
C. binding location
D. active site
28.Which of the following kinetic parameters best describes how well suited a speci c
compound functions as a substrate for the enzyme?
 (a) Km
(b) Vmax
(c) kcat
(d) kcat/Km
29. AST and ALT used diagnosis. What vitamin is important for these enzymes activity?
(A) vitamin pp
(B) folic acid
(C) ribo avin
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8/4/2018 MCQ module 1
(C) ribo avin

Biochemistry
(D) niacin
(E) thiamin
30. A ne chromatography method with use of special ligand placed on the carrier proposed to
obtain from pancreas of animals’ enzyme amylase preparation. Name substances that may be
used as the ligand:
(A) Glucose
(B) Starch
(C) Sucrose
(D) Cellulose
(E) Lactose
31. Enzyme catalyzes the transfer of structural fragment from one substrate to other one to
form two products. Name the class of this enzyme.
(A) Hydrolase
(B) Isomerase
(C) Transferase
(D) Oxidoreductase
(E) Ligase
32. The most likely effect of a non-competitive inhibitor on an Michaelis-Menten enzyme is to
A. Increase the Vmax
B. Decrease the Vmax
C. Increase both the Vmax and the Km
D. Decrease both the Vmax and the Km
33. The human species can biosynthesize
(A) Vitamin C
(B) Vitamin B12
(C) Thiamine
(D) Niacin
34. Digestive enzymes are
(P) Lyases
(Q) Oxidoreductase
(R) Isomerases
(S) Hydrolases
(T) Transferases
35. The increase of activities for enzymes LDH1, LDH2, AsAT, creatine phosphate kinase was
checked in the blood serum of diseased person. Name the probable organ (tissue) where
pathology was developed:
A. Heart muscle (beginning of myocardium infarction)
B. Skeletal muscle (dystrophy or atrophy)
C. Kidney and adrenal gland
D Connective tissue
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D. Connective tissue
Biochemistry
E. Liver and kidney
36. There was revealed acute increase of AsAT activity in the blood serum of patient for whom
in 12 hours before acute attack of chest pain was checked. Find out the pathology whose
development is associated with those change in the blood:
A. Myocardium infarction
B. Viral hepatitis
C. Collagenosis
D. Diabetes mellitus
E. Diabetes insipidus
37. Sick man 49 years old (a driver by profession) complains of unbearable retrosternal pain
that was found in the cervical 2 hours ago. It is severe state, the patient has pale face, and, and
his heart sounds are weakened. The laboratory investigation revealed high activity of Creatine
phosphate kinase and LDH1. Name the disease which may by observed in patient:
A. Acute myocardium infarction
B. Acute pancreatitis
C. Stenocardia
D. Cholestasis
E. Diabetes mellitus
38. Fibrinolytic drugs can dissolve formed in human’s blood thrombus. Choose those
preparation:
(A) . Ribo avin
(B) Streptokinase .
(C) Isoniazide
(D) Phenobarbital
(E) Vicasol
39. Increased total activity of LDH is found in the blood plasma of patient. It may be at
disorders associated with diseases of heart, kidney and liver. Name additional clinical test to
make differentiation in diagnosis of this patient:
A. LDH isozymes content determination in blood plasma
B. Glucose content determination in the blood
C. Ketone bodies determination in the blood plasma
D. Total cholesterol determination in blood plasma
E. Amylase activity determination in blood plasma
40. The best test for acute pancreatitis in the presence of mumps is
(A) A serological test for mumps
(B) Serum amylase
(C) Urinary amylase
(D) Serum lipase
41. In the graphs shown below, in which the initial rate of an enzyme-catalyzed reaction was
plotted against the substrate concentration the solid line represents data obtained in the
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plotted against the substrate concentration, the solid line represents data obtained in the
Biochemistry
absence of any drug while the dotted line represents data obtained in the presence of the drug
X. Which of the following statements about this system is TRUE?

A) Drug X increases the maximal velocity of the enzyme.

B) Drug X acts as a positive effector of the enzyme.
C) Drug X acts as a negative effector of the enzyme.
D) Drug X has no effect on the enzyme.
E) The enzyme exhibits simple Michaelis-Menten kinetics in the absence of Drug X.
42. Zn+2 is an inorganic activator for enzyme.
(A) Carbonic anhydrase
(B) Phosophatase
(C) Chymotrypsin
(D) Maltase
43. Proserin preparation is reversible inhibitor of acetylcholine esterase. Find out mechanism
of proserin (P) action as inhibitor:
A. Competition of P with acetylcholine to attach active site of enzyme
B. Enzyme denaturation by P
C. Covalent modi cation of active site of enzyme by P
D. Fe oxidation in active site of enzyme by P
E. Covalent modi cation of allosteric site of enzyme by P
44. Treatment of methanol poisoning of a patient is the use of ethanol as preparation (per os
or intravenously) in a quantity that can cause for healthy person toxici cation. Find out
explanation for effective ethanol use as drug in this case:
A. The a nity of ethanol to active site of alcohol dehydrogenase is much higher than for
methanol
B. Ethanol is allosteric inhibitor of alcohol dehydrogenase
C. Ethanol blocks the coenzyme of alcohol dehydrogenase
D. Ethanol is destroyed to form more toxic compounds as methanol
E. Ethanol suppresses diffusion of methanol
45. The use of colors with high content of lead is limited in production of toys for kids. Lead
ions toxicity is explained from their in uence as inhibitors for enzymes. Name type of
inhibition for lead ions:
A. Non-competitive
B. Competitive
C. Uncompetitive
D. Allosteric
E. Irreversible
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Biochemistry
I. Connect enzyme and its tissue location
1. LDH 1
A. Brain
2. LDH 5
B. Panreas cancer
3. BB-CPK
C. Heart
4. Nagao Al Phosphatase
D. Prostatic cancer
5. Acid Phosphatase
E. Liver

II. A 32- year- old male reported to emergency with active bleeding from nose.  History
revealed that he had been on Orlistat for weight reduction from the past two years. He had
started Orlistat without the advice of any practitioner and  had lost nearly 20 kg of body weight
.There was no history of hypertension, bleeding disorder or any other medical illness. No such
bleeding episode occurred in the past.
There was no abnormality detected upon local examination of nose.  What is the relationship
between Orlistat and epistaxis?

III. Why is it said that babies undergoing phototherapy for physiological jaundice are prone to
develop ribo avin de ciency?

Biosyn March 7, 2018 at 4:29 AM

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