BIOSTAT ® B plus

Laboratory
fermentor |bioreactor
benchtop system

Engineering by
Sartorius BBI Systems
BIOSTAT® B plus integrated … ready to use packages for your drug discovery
system solution… and small-scale-production

BIOSTAT® B plus are designed to become the

Features
new benchtop systems standard in research
(Single or Twin configuration)
fermentors and bioreactors, worldwide.
• Graphical user interface with touch
Application driven preconfigured packages
screen
for microbial culture and cell culture can be
• Trend display with up to 6 process
delivered ready to use right “out of the box”.
values
• Direct balance connection
A comprehensive range of pre configured
• 1 L–10 L UniVessel® culture vessel
packages are available to satisfy the demands
with storage bottle tray, lifting
of both microbial & cell culture applications.
handles and sampling system
• High performance stirrer motor for
Basic unit comprising…
all applications and UniVessel® sizes
– Stainless steel housing
• Up to 4 integrated peristaltic pumps,
– Digital controller
2 external pump connections
– Operating interface
• Application driven integrated
– Gassing system with rotameter, solenoid
gassing system, choice of:
valves or mass flow controller
– microbial culture
– Motor with controller
– O2 Enrichment
– Thermostat system with circulation pump
– Gas Flow Ratio Control
or dry heating with controlled cooling
– cell culture|dual use
water valve
– Exclusive Flow
– Up to 4 peristaltic pumps
– cell culture
– Integrated amplifier
– Additive Low Flow
• Integrated thermostat or
Culture vessel equipped with…
dry heating system
– Sensors for temperature, pH, DO,
• Space for Redox and turbidity
foam and level
measurement (Single only)
– Stirrer shaft with industrial sealing
• Pre-configured software for system
– Impeller
extensions
– Aeration tube with sparger, sterile filters
and exhaust cooler
– Storage bottles, sample/harvest pipe, MFCS/DA Software
blind plugs To accelerate your research activities,
– Tube, O-ring and tool kit a powerful supervisory software MFCS/DA for
extended visualization, data acquisition and
…and optionally a full range of accessories trend display is included.
to meet your future needs.

Space requirement
Required bench size approx. W + H + D (mm) Single Twin
®
BIOSTAT B plus / 1 L 560 + 730 + 565 800 + 730 + 565
®
BIOSTAT B plus / 2 L 590 + 730 + 565 860 + 730 + 565
®
BIOSTAT B plus / 5 L 620 + 730 + 565 920 + 730 + 565
BIOSTAT® B plus / 10 L 670 + 820 + 565 1040 + 820 + 565
Required inner autoclave dimension d + H [mm]
UniVessel® 1 L 240 + 500 *
®
UniVessel 2 L 270 + 550 *
UniVessel® 5 L 300 + 700 *
®
UniVessel 10 L 350 + 820 *
Utility lines Customer supply
Power supply 230 VAC or 120 AC
Gasses Controlled @ 1.5 barg; dry, particle and oil free
Water Controlled @ 2 barg
Drain @ 0 barg
* Height reducible via flexible adaptor
Digital Controller Gassing Culture vessel
BIOSTAT® B plus controller created for the Integrated culture vessel protection UniVessel® autoclavable culture vessels.
needs of today’s bioprocess applications. via safety valve Developed with over 40 years experience
in sterile design.
– Single and Twin control capability O2-Enrichment
– Graphical user interface with color display – For microbial cultures – 1 L – 10 L jacketed or single wall
and touch screen – Automatic gasmixing of Air and O2 culture vessels
– Integrated amplifiers for temperature, pH, – Solenoid valve for O2-Enrichment – Pre-configured for microbial or cell
DO, foam & level capability culture application
– Twin combined Level |Foam controller – Controlled via DO controller – Stirrer shaft with single
– Space for Redox and turbidity amplifier, – Exchangeable Rotameter mechanical seal
Single only – Optional massflow controller – Polished head plate for highest
– Integrated digital control loops for temper- sanitary conditions
ature, pH, DO, agitation, gasmixing, air flow Gas Flow Ratio Control – Vertical lifting handles for easy handling
and 2+ substrate – For microbial cultures – Head plate with maximized numbers
– Level control via probe or balance – Gasmixing of Air and O2 of ports
– Multi-stage DO cascade control via Gas Flow Ratio Controller – Removable addition bottle support
– Totalizer with digital calibration for probes – Two integrated massflow controller – Minimized autoclave space requirement
and pumps for Air and O2 – 316 L stainless steel for medium
– In-process pH-recalibration – Controlled via DO controller contact parts
– Trend display for up to 6 process values – Exchangeable Rotameter – Real O-ring sealing, no compressed
– Balance connection O-rings
– Developed according to GAMP guidelines Exclusive Flow – Full range of accessories for microbial
– For cell culture or multipurpose use and cell culture applications
Temperature Control System – Sparger and Overlay gas outlet
Choice of dry heating or thermostat system – Automatic gasmixing of Air, O2, N2, CO2 Stirrer Drive
for precise temperature control with rapid for Sparger aeration High performance servo drive combines low
heating and cooling rates. – Air for Overlay aeration shear agitation for cell cultures with high
– 2 exchangeable rotameters speed mixing for microbial high cell density
Thermostat System – Controlled via pH/DO controller fermentations.
– Integrated in basic unit – Optional massflow controller
– Powerful heater (1 kW) – Speed range 20–2.000 rpm
– Automatic controlled cooling water valve Additive Low Flow – Maintenance free
– Circulation pump – For cell cultures – High torque
– Temperature range 8 °C above cooling water – Sparger and Overlay gas outlet – Easy handling
up to 80°C. – Automatic gasmixing of O2, N2, CO2
for Sparger gassing
Dry Heating System – Air for Overlay gassing
– Integrated in basic unit – 4 exchangeable rotameters
– Plug connector for heating blanket – Controlled via pH/DO controller
– Automatic controlled cooling water valve – Optional mass flow controller for total
for optional cooling finger Sparger and Overlay flow
– For temperatures up to 60°C
Pumps
Controlled via BIOSTAT® B plus controller
for precise media conditioning, feeding and
harvest.
– Up to 4 integrated pumps
– Configurable substrate controller
– Up to 2 external or internal feed pumps
– Watson Marlow pump heads
MFCS SCADA Software Family …
the Software Solution for your Bioprocess

MFCS/win 2.1
MFCS/win 2.1 the 4th generation of
BBI’s enhanced Supervisory Control
and Data Acquisition software package
satisfies a broad range of requirements
in research, pilot and production area.
Supplied preconfigured and “ready to
use”, the configuration can easily be
adapted by the user to any change in
process requirement. Main features
include on-line data acquisition and
storage, sample data management for
off-line measured values, process evalu-
ation and visualisation, as well as event
dependent process control, process
documentation via data export and
batch reporting following the cGMP
rules. The open system architecture
provides connectivity to a wide range of
3rd party devices and software packages
– via MFCS/win drivers, OPC-Client,
OPC-Server or programmable interface
(API).

Fully validatable according to GMP

Our key platform to automation is based on Operator Service category 4 (Configurable System)
the use of flexible Digital Control Systems Batch Display provides an overview of all and 21 CFR Part 11 compliance for
(Micro-DCU and DCU), which are specifically process units. Additional on-line, config- electronic records and signatures,
tailored for fermentation and cell culture urable views display for individual process MFCS/win has all the necessary func-
applications. When combined with our Super- units as well as an overall complete system tionality for operation in a regulated
visory Control and Data Acquisition (SCADA) display. cGMP production environment.
systems, this solution provides the most
cost-effective platform for a broad range Sample Data Management
of applications. During a batch, off-line sample analysis
generates critical process data, which should
The current SCADA Software Family be included in the batch record. These values
consists of two products: MFCS/DA and can be entered into a standard spreadsheet/
MFCS/win… table.

MFCS/DA Plotting
MFCS/DA has been designed as an self- Any combination of 1 to 10 variables can be
explanatory system with plug and play displayed together. On-line data, as well as
configurations for micro-DCU based fermen- manually entered, off-line data can be
tors like the BIOSTAT® B plus. It provides selected. Line types, colors and other plot
simultaneous data acquisition and control characteristics are user-definable. Preferences
from up to 4 process units. The Operator can be stored in re-usable plotting templates.
Service program acts as the main point of
interaction with the software, providing Data Export
access to all functions: set-up, control, Data from finished or still active bioprocesses
plotting, etc. can be further processed by 3rd party soft-
ware using the Export Module.
MFCS/DA even includes the ability to incor-
porate other laboratory data, such as off-line Programming Interface
process analysers. An Export function gives Typical applications of this module could
added flexibility for analysing data using include calculation of Oxygen Uptake Rates
other off-line programs. More advanced (OUR), Carbon Dioxide Evolution Rate (CER)
control strategies can be developed by the and Respiration Coefficient (RQ) for use in
already included Programmer´s Interface. establishing new controller setpoints for more
advanced process control.
… Additional Accessories for Today’s and Future Bioprocessing Needs

Perfusion system: Internal Spinfilter Perfusion system: External Spinfilter Bubble free aeration
The Internal Spinfilter is a scalable perfusion The External Spin Filter (ESF) is a scalable Bubble free aeration is recommended
system which is applicable for continuous perfusion system applicable for continuous for extremely shear stress sensitive
medium exchange for long-term cultivation. medium exchange for long-term cultivation. cells, such as primary mammalian cells.
It can be used for suspension or micro carrier It is operated in an external loop with Shear forces caused by bursting bubbles
cultivations and is available in stainless steel adjustable rotation speed. can be avoided and, in addition foam
or disposable design. production is prevented.

It is directly mounted on the stirrer shaft,

resulting in easy sterile handling and
operation.

SuperSpinner Turbidity measurement system:

The SuperSpinner is a incubator-based cell FUNDALUX® II
cultivation system. It combines a traditional FUNDALUX® II is an absorption-based
spinner bottle with a patented bubble free photometric probe, designed for use
Sterile couplings: STT couplings aeration|stirrer system. SuperSpinners are with fermentors|bioreactors.
STT-Sterile quick connectors provide sterile used for seed cultivations and micro scale
connection of tubings. Due to the use of a production and are applicable for suspension By continuously measuring cell growth|
replaceable slot membranes STT couplings and micro carrier cultures. Due to the dispos- biomass as a function of light absorp-
provide a re-usable sterile environment. able design of the aeration|stirrer system tion, the process operator can gain
constant cultivation results are assured. real-time knowledge to optimize the
process control.
Sartorius BBI Systems GmbH
Postfach (P.O.B.) 1363
34203 Melsungen
Germany
Schwarzenberger Weg 73–79
34212 Melsungen
Germany
Phone +49.5661.71.3400
Fax +49.5661.71.3702

Sartorius BBI Systems, Inc.

2800 Baglyos Circle
Bethlehem, PA 18020
USA
Phone +1.610.866.4800
Fax +1.610.866.4890

Sartorius BBI Systems s.r.l.

Via Vigliani 13
20148 Milano
Italy
Phone +39.02.4817.327
Fax +39.02.4983.176

Sartorius India Pvt. Ltd.

10, 6th main, 3rd Phase Peenya
KIADB Industrial Area
Bangalore – 560 058
India
Phone +91.80283.91963
Fax +91.80283.98262

www.sartorius-bbi-systems.com
[email protected]

Specifications subject to change.

Printed in Germany on paper that has been
bleached without any use of chlorine.