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Albumin: Instrument

1. This document provides the application parameters and instrument settings for measuring albumin levels using a spectrophotometric bromocresol green method on an ARCHITECT C8000 instrument. 2. The reagent is ready to use and measures albumin levels in a range of 0.0-70.0 g/L using a single wavelength of 628/700 nm with a reading time of 7-9 seconds. 3. Calibration is done using a linear method with a single calibrator at a concentration to be determined by the user and run in triplicates every 999 hours or full interval.

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Enrique Duarte
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34 views

Albumin: Instrument

1. This document provides the application parameters and instrument settings for measuring albumin levels using a spectrophotometric bromocresol green method on an ARCHITECT C8000 instrument. 2. The reagent is ready to use and measures albumin levels in a range of 0.0-70.0 g/L using a single wavelength of 628/700 nm with a reading time of 7-9 seconds. 3. Calibration is done using a linear method with a single calibrator at a concentration to be determined by the user and run in triplicates every 999 hours or full interval.

Uploaded by

Enrique Duarte
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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ALBUMIN

Spectrophotometric
BROMOCRESOL GREEN

The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the
instrument. It is advisable to validate the use when there is any change in software or reagent versions.

Instrument: ARCHITECT C8000


Reagent preparation
Reagent is ready to use.

Instrument settings

General Conventional Units


Assay ALB Type Photometric Results Unit g/L
Number ... Version 1 Assay Defaults Low Linearity 1.4
High Linearity 70
Reaction Definition
Reaction Mode End Up Read Times Gender and Age specific Ranges
Wavelength 628 / 700 Main 7 -- 9 GENDER AGE (Y) NORMAL
Last Required Read 9 Flex __ -- __ Male 0-130 35 - 50
Absorbance Range … / … Color Correction __ -- __ Female 0-130 35 - 50
Sample Blank Type None Blank Read Time __ -- __ Either 0-130 35 - 50

Reagent / Sample R1 R2 Reults Units g/L


Reagent ALB Reagent Volume 300 Decimal Places 1 (Range 0-4)
Diluent Saline Water Volume __ __ Correlation Factor 1.0000
Diluent Disp. Mode Type 0 Dispense Mode Type 0 Type 0 Intercept 0.0000

Diluent Name Sample Water Dilution Factor


Standard 3 1:1.00

Validity Checks
Reaction definition None
Rate Linearity % ___
Calibration
Method Linear

Calibrators and Volumes


Calibrator Set Standard Calibrator Level Concentration Volume
Blank 0.0 3
Std. 1 (*) 3
Std. 2 -- --
Std. 3 -- --
Std. 4 -- --
Replicates 3 (Range 1-3) Std. 5 -- --

Intervals Validity Checks


Calibration Interval Full Interval 999 hours Blank Absorbance range: 0.0000 -- 0.0000
Calibration Type Adjust Type None Span Absorbance Range: 0.0000 -- 0.0000
Expected cal Factor : 0.00
Expected Cal factor tolerance: %0
(*) Enter the standard value
… Values to be entered by the user
Version 0707

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