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DIfference Nutrient Agar and Nutrient Broth

Nutrient Agar is a general purpose growth medium suitable for cultivating all non-fastidious bacteria. It contains peptone, beef extract, agar, and yeast extract to support growth, as well as sodium chloride. While Nutrient Agar can support most bacteria, more specialized media like Columbia Agar or Blood Agar are preferred for cultivating fastidious organisms. Nutrient Agar is prepared by suspending the powder in water, autoclaving, and pouring into containers.

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Annisa Azzahroh
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308 views1 page

DIfference Nutrient Agar and Nutrient Broth

Nutrient Agar is a general purpose growth medium suitable for cultivating all non-fastidious bacteria. It contains peptone, beef extract, agar, and yeast extract to support growth, as well as sodium chloride. While Nutrient Agar can support most bacteria, more specialized media like Columbia Agar or Blood Agar are preferred for cultivating fastidious organisms. Nutrient Agar is prepared by suspending the powder in water, autoclaving, and pouring into containers.

Uploaded by

Annisa Azzahroh
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Nutrient Agar

DM 179 A general purpose growth medium. Description

Typical formula* grams per litre Nutrient Agar is an inexpensive medium suitable
for the cultivation of all bacteria which are non-
Peptone A 6.0 exacting in their growth requirements. It
Beef extract (RM20) 1.0 functions therefore, as a general purpose
Agar A (RM 10) 14.0 medium for the maintenance of stock cultures
Yeast extract 2.0 and as a useful demonstration and teaching aid.
Sodium chloride 5.0 Adequate salt and agar concentrations permit the
pH approx.7.3 addition of blood, serum or other enriching fluids
where these are required. However for the
Directions successful growth of fastidious organisms, a
medium such as Columbia Agar (DM115), Blood
1. Suspend by swirling 28g of powder in 1 litre or Agar Base (DM100) or Blood Agar Base Special
the contents of the sachet in the stated (DM101) is to be preferred.
volume of distilled or deionised water.
2. Autoclave at 121ºC (15 p.s.i.) for 15 minutes.
3. Mix well before pouring.

Nutrient Broth
DM 180 A general purpose liquid medium for the Description
cultivation of fastidious organisms.
MAST Nutrient Broth is designed to give good
Typical formula* grams per litre growth from minimal inocula of such organisms
as coliforms and staphylococci. With the addition
Peptone A (RM50) 10.0 of serum, the broth is ideal for the cultivation of
Sodium chloride 5.0 fastidious organisms. It is also suitable for the
Beef extract (RM20) 10.0 preservation of stock cultures. The formula
pH approx. 7.3 complies with the recommendations laid down in
1
the British Pharmacopoeia for media for sterility
Directions testing of aerobic organisms.

1. Dissolve 25g of powder in 1 litre or the References


contents of the sachet in the stated volume of
distilled or deionised water. 1. British Pharmacopoeia 1968; p. 1357.
2. Dispense the solution into suitable containers.
3. Autoclave 121ºC (15p.s.i.) for 15 minutes.

MAST is a Registered Trademark


*Formulation may change to meet performance criteria PG 4/97 V0.1

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