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1. The document provides instructions for validating a protein assay on an ARCHITECT C8000 instrument using a biuret reagent. 2. It lists the instrument settings, reaction definition, reagent and sample details, calibration information including calibrators and intervals for the protein assay. 3. Validation of the protein assay involves checking that the instrument settings, reagent parameters and calibration meet the specified criteria.

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Enrique Duarte
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31 views

Prot

1. The document provides instructions for validating a protein assay on an ARCHITECT C8000 instrument using a biuret reagent. 2. It lists the instrument settings, reaction definition, reagent and sample details, calibration information including calibrators and intervals for the protein assay. 3. Validation of the protein assay involves checking that the instrument settings, reagent parameters and calibration meet the specified criteria.

Uploaded by

Enrique Duarte
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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PROTEIN

Spectrophotometric
BIURET

The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the
instrument. It is advisable to validate the use when there is any change in software or reagent versions.

Instrument: ARCHITECT C8000


Reagent preparation
Reagent is ready to be used.

Instrument settings

General Conventional Units


Assay PROT Type Photometric Results Unit g/L
Number ... Version 1 Assay Defaults Low Linearity 4.6
High Linearity 150
Reaction Definition
Reaction Mode End Up Read Times Gender and Age specific Ranges
Wavelength 548 / 0 Main 31 -- 33 GENDER AGE (Y) NORMAL
Last Required Read 33 Flex __ -- __ Male 0-130 64 - 83
Absorbance Range … / … Color Correction __ -- __ Female 0-130 64 - 83
Sample Blank Type None Blank Read Time __ -- __ Either 0-130 64 - 83

Reagent / Sample R1 R2 Reults Units g/L


Reagent PROT Reagent Volume 200 Decimal Places 1 (Range 0-4)
Diluent Saline Water Volume __ __ Correlation Factor 1.0000
Diluent Disp. Mode Type 0 Dispense Mode Type 0 Type 0 Intercept 0.0000

Diluent Name Sample Water Dilution Factor


Standard 4 1:1.00

Validity Checks
Reaction definition None
Rate Linearity % ___
Calibration
Method Linear

Calibrators and Volumes


Calibrator Set Standard Calibrator Level Concentration Volume
Blank 0.0 4
Std. 1 (*) 4
Std. 2 -- --
Std. 3 -- --
Std. 4 -- --
Replicates 3 (Range 1-3) Std. 5 -- --

Intervals Validity Checks


Calibration Interval Full Interval 999 hours Blank Absorbance range: 0.0000 -- 0.0000
Calibration Type Adjust Type None Span Absorbance Range: 0.0000 -- 0.0000
Expected cal Factor : 0.00
Expected Cal factor tolerance: %0
(*) Enter the standard value
… Values to be entered by the user
Version 0707

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