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Creatine Kinase: Instrument

This document provides instrument settings and parameters for measuring creatine kinase using a continuous spectrophotometric assay on an AU 400 or AU 640 analyzer. It specifies settings like reagent volumes, wavelengths, measuring points, linearity range, calibration type and factors to validate the assay. The working reagent is prepared by mixing contents of bottle B into bottle A and is stable for 15 days at 2-8°C.

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Enrique Duarte
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68 views

Creatine Kinase: Instrument

This document provides instrument settings and parameters for measuring creatine kinase using a continuous spectrophotometric assay on an AU 400 or AU 640 analyzer. It specifies settings like reagent volumes, wavelengths, measuring points, linearity range, calibration type and factors to validate the assay. The working reagent is prepared by mixing contents of bottle B into bottle A and is stable for 15 days at 2-8°C.

Uploaded by

Enrique Duarte
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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CREATINE KINASE

Continuous-spectrophotometric
IFCC

The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the
instrument. It is advisable to validate the use when there is any change in software or reagent versions.

Instrument: AU 400 / 640


Reagent preparation
Working Reagent: Empty the contents of a Reagent B bottle into a Reagent A bottle. Swirl gently.
Stable for 15 days at 2-8 ºC.

Instrument settings

Specific Test Parameters Min OD Max OD


Sample: Volume 10 µL Dilution 0 µL L 0.0 H 1.0
Reagents: R1 Volume 250 µL Dilution 0 µL Reagent OD limit:
Wavelength: Pri. 340 Sec. 380 First L 0.0 First H 0.4
Method: RATE Last L 0.0 Last H 0.4
Reaction slope: + Dynamic Range:
Measuring Point 1: First 9 Last 18 L 0 H 1200
Measuring Point 2: First 0 Last 0 Correlation Factor:
Linearity: 15 % A 1 B 0
No Lag Time: NO On-board stability period:

L H
7. None selected
8. Out of range 24 195
L H
Panic Value Unit: U/L Decimal places: 0

Calibration Specific

Calibration Type: MB Formula: Y=AX+B Counts: 2 Process: CONC.

Cal. Nº OD CONC Factor/OD-L Factor/OD-H


Point 1: # * -9999 9999

MB Type Factor: 6878

* Enter the assigned standard value


# User defined

Vers. 0206

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