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Albumin: Instrument

This document provides instrument settings and parameters for analyzing albumin levels using a spectrophotometric bromocresol green method on a BS-300 instrument. It specifies reagent preparation, instrument settings like wavelengths, volumes, and incubation times. It also outlines calibration details including using a linear method and entering standard values. Maintaining validated parameters when software or reagents change is advised.

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Enrique Duarte
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55 views

Albumin: Instrument

This document provides instrument settings and parameters for analyzing albumin levels using a spectrophotometric bromocresol green method on a BS-300 instrument. It specifies reagent preparation, instrument settings like wavelengths, volumes, and incubation times. It also outlines calibration details including using a linear method and entering standard values. Maintaining validated parameters when software or reagents change is advised.

Uploaded by

Enrique Duarte
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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ALBUMIN

Spectrophotometric
BROMOCRESOL GREEN

The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the
instrument. It is advisable to validate the use when there is any change in software or reagent versions.

Instrument: BS-300
Reagent preparation
Reagent is ready to use.

Instrument settings

PARAMETERS

Nº … Test ALB Method End-Point Direction Ascend


Unit g/L Decimal 0 Prim.Wavelength 630 Sec. Wavelength 700
Sample Volume 3 R1 Volume 300 R2 Volume 0 Line Limit N/A

Response
Incubation -- Check Antigen No Lower -- Sample Volume …
Reaction 0 -- 10 Substrate N/A Upper -- Dilution …

R1 Blank Mix R Blank Linearity Full Name …


Lower … Lower … Lower 1.4 Print Number …
Upper … Upper … Upper 70

Calibration
Method Linear Std. 0 0
Std. 1 (*)
Std. 2 --
Std. 3 --
Std. 4 -- (*) Enter the standard value
Std. 5 -- … Value entered by the user

Version 0707

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