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TLC Simulator

This document provides instructions for using a TLC simulator to separate mixtures of hexane and ethyl acetate. The simulator is used to observe how changing solvent composition affects Rf values. More polar solvents like pure ethyl acetate decrease Rf values compared to less polar solvents like pure hexane, indicating that more polar compounds have lower Rf in a given solvent system.

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880 views

TLC Simulator

This document provides instructions for using a TLC simulator to separate mixtures of hexane and ethyl acetate. The simulator is used to observe how changing solvent composition affects Rf values. More polar solvents like pure ethyl acetate decrease Rf values compared to less polar solvents like pure hexane, indicating that more polar compounds have lower Rf in a given solvent system.

Uploaded by

Krys tall
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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TLC

 Simulator  Worksheet                  Dr.  Laurie  Starkey,  Cal  Poly  Pomona    


 
Follow  instructions  to  explore  the  TLC  simulator  and  answer  the  questions  below.  
 
1)  Click  “Start  Experiment”  to  run  the  simulator  with  a  50:50  mixture  of      
Hexane  and  Ethyl  Acetate.    Sketch  the  plate  below  (label  the  spots  red  and  blue)  
                                                 http://goo.gl/PZSCpb    
    Is  silica  gel  polar?  
Which  compound  is  more  polar,  red  or  blue?  Explain  briefly.  
 
           
    Draw  the  structures  for  ethyl  acetate  (EtOAc):                and  hexane:                                                                                
 
2)  Click  on  “reset  experiment”  and  change  the  solvent  system  on  one  of  the  plates  to  be    
75%  Ethyl  Acetate.    Sketch  the  plate  below  (label  spots)  and  answer  the  given  questions.  
 
    Is  the  new  solvent  (compared  to  50:50)  more  or  less  polar?    Explain  briefly.  
 
 
 
  What  happened  to  the  Rf  of  the  spots  in  the  new  solvent?  Explain  briefly.  
 
 
 
3)  Click  on  “reset  experiment”  and  change  the  solvent  system  on  one  of  the  plates  to  be    
25%  Ethyl  Acetate.    Sketch  the  plate  below  (label  spots)  and  answer  the  given  questions.  
 
    Is  the  new  solvent  (compared  to  50:50)  more  or  less  polar?    Explain  briefly.  
 
 
 
  What  happened  to  the  Rf  of  the  spots  in  the  new  solvent?  Explain  briefly.  
 
 
 
4)  Click  on  “reset  experiment”  and  change  the  solvent  systems  to  be    
100%  Ethyl  Acetate  on  one  plate  and  100%  Hexane  on  the  other.      
Sketch  the  plates  below  and  describe  the  Rf  of  the  spots.  
     
  Conclusions  about  silica  gel  TLC  (circle  the  correct  answers)  
   
  A  more  polar  compound  has  a  higher/lower  Rf.  
  A  less  polar  compound  has  a  higher/lower  Rf.  
 
  A  more  polar  solvent  causes  a  higher/lower  Rf.  
  A  less  polar  solvent  causes  a  higher/lower  Rf.  
100%  EtOAc                  100%  hexane  
Rf  =                      Rf  =   To  increase  the  Rf  of  a  spot,  you  should  add  more  hexane/EtOAc.  

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