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Chapter 8 Homework

This document describes a homework assignment on recombinant DNA technology and the polymerase chain reaction (PCR). Students are asked to label diagrams of these processes and answer multiple choice questions to identify the key steps and applications. For recombinant DNA technology, these include using restriction enzymes to cut DNA, inserting a gene of interest into a plasmid, and inserting the plasmid into a bacterial cell. For PCR, the key steps are denaturation, annealing of primers, and extension of DNA through repeated cycles. Applications include diagnosing diseases and generating DNA for cloning.

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93 views

Chapter 8 Homework

This document describes a homework assignment on recombinant DNA technology and the polymerase chain reaction (PCR). Students are asked to label diagrams of these processes and answer multiple choice questions to identify the key steps and applications. For recombinant DNA technology, these include using restriction enzymes to cut DNA, inserting a gene of interest into a plasmid, and inserting the plasmid into a bacterial cell. For PCR, the key steps are denaturation, annealing of primers, and extension of DNA through repeated cycles. Applications include diagnosing diseases and generating DNA for cloning.

Uploaded by

Kvn4N6
Copyright
© © All Rights Reserved
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3/31/2019 Chapter-08_Homework

Chapter-08_Homework
Due: 1:59pm on Friday, February 22, 2019
You will receive no credit for items you complete after the assignment is due. Grading Policy

Figure 8.1

You will identify the steps in recombinant DNA technology.

Part A
Label the processes involved in recombinant DNA technology.

Drag the appropriate labels to their respective targets.


ANSWER:

Reset Help

Bacterial Plasmid
chromosome
Gene of interest
Isolate plasmid
Enzymatically
cleave DNA into
fragments

Isolate fragment
with the gene of
interest

Insert gene into


plasmid

Insert plasmid and


gene into
bacterium

Correct

Visualize It! Chapter 8 Figure 1

You will identify the steps and components of the polymerase chain reaction.

Part A
Label the steps and components of the polymerase chain reaction.
Drag the appropriate labels to their respective targets.

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3/31/2019 Chapter-08_Homework
ANSWER:

Reset Help

Denaturation (94
degrees C)

DNA primer

Priming (65 degrees C) Deoxyribonucleotide


triphosphates

DNA polymerase

Repeat

Extension (72 degrees


C)

Correct

Microbiology Animation: Polymerase Chain Reaction (PCR): Overview

Click here to complete this activity.

Then answer the questions.

Part A

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3/31/2019 Chapter-08_Homework
What is the end goal of PCR?
ANSWER:

To allow cells to make DNA faster, thereby growing faster

To quickly increase the number of copies of a specific DNA sequence

To increase the pool of different DNA sequences

Correct

Part B
PCR stands for

ANSWER:

polymerase chain reaction.

polymerase copy reaction.

polymerization copying rapidly.

Correct

Part C
Which of the following is an application that uses PCR?
ANSWER:

Diagnosing a disease

Providing enough DNA for cloning into another organism

Sequencing a gene, diagnosing a disease, and providing enough DNA for cloning into another organism

Sequencing a gene

Correct

Microbiology Animation: Recombinant DNA Technology

Click here to complete this activity.

Then answer the questions.

Part A
How do restriction enzymes cut DNA sequences?
ANSWER:

They cut DNA at sites, called recognition sites, that have specific nucleotide sequences.

They have the ability to cut DNA randomly.

They cut DNA at sequences that have lots of adenine bases.

https://session.masteringmicrobiology.com/myct/assignmentPrintView?assignmentID=1843207 3/4
3/31/2019 Chapter-08_Homework

Correct

Part B
In general, how might recombinant DNA technology be used to prevent a genetic disorder caused by a mutation in a single gene?

ANSWER:

To insert a desirable gene, remove an undesirable gene, or replace a defective gene with a functioning gene

To remove an undesirable gene

To replace a defective gene with a working gene

To insert a desirable gene

Correct

Part C
Which of the following attaches the target gene to a desired location?
ANSWER:

Restriction enzymes

Chromosomal DNA

Plasmids

DNA ligase

Correct

Part D
Why would a recombinant DNA molecule be inserted into a host cell?
ANSWER:

Restriction enzymes can only be used inside of a cell.

It can be copied, transcribed, and translated into a desired protein.

It can protect the recombinant DNA.

Plasmids cannot be isolated outside of a host cell.

Correct

Score Summary:
Your score on this assignment is 100%.
You received 2 out of a possible total of 2 points.

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