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Rheumatoid Factors: Instruments

This document provides instructions for using a latex turbidimetry assay to measure rheumatoid factors on clinical chemistry analyzers. It details the reagent preparation and storage, instrument settings including sample and reagent volumes, wavelengths, reaction kinetics parameters, calibration details and the assay's reportable range. The guidelines are meant to help validate the reagents and ensure consistent performance.

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Enrique Duarte
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71 views

Rheumatoid Factors: Instruments

This document provides instructions for using a latex turbidimetry assay to measure rheumatoid factors on clinical chemistry analyzers. It details the reagent preparation and storage, instrument settings including sample and reagent volumes, wavelengths, reaction kinetics parameters, calibration details and the assay's reportable range. The guidelines are meant to help validate the reagents and ensure consistent performance.

Uploaded by

Enrique Duarte
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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RHEUMATOID FACTORS

Turbidimetry
LATEX
Non linear calibration

The application parameters comprised here constitute a guide to facilitate the validation of our reagents by
the instrument. It is advisable to validate the use when there is any change in software or reagent versions.

Instruments: AU 400 / 640

Reagent preparation
Reagent 1: use the Diluent. Stable for 28 days at 2-8 ºC.
Reagent 2: use the Latex. Shake the Latex vial gently before using.

Instrument settings
Specific Test Parameters
Sample: Volume 3 µL Dilution 0 µL
Reagents: R1 Volume 240 µL Dilution 0 µL Reagent OD limit:
R2 Volume 60 µL First L -2.0 First H 2.5
Wavelength: Pri. 660 Sec. Last L -2.0 Last H 2.5
Method: END
Reaction slope: + Dynamic Range:
Measuring Point 1: First 0 Last 20 L 0 H #
Measuring Point 2: First 0 Last 12 Correlation Factor:
Linearity: % A 1 B 0
No Lag Time: On-board stability period:

L H
7. None selected
8. Out of range 0 30
L H
Panic Value Unit: IU/mL Decimal places: 1

Calibration Specific

Calibration Type: 5AB Formula: POLYGONAL Counts: 2 Process: CONC.

Cal. Nº OD CONC Factor/OD-L Factor/OD-H


Point 1: # * -2.0 2.5
Point 2: # * -2.0 2.5
Point 3: # * -2.0 2.5
Point 4: # * -2.0 2.5
Point 5: # * -2.0 2.5

* Enter the assigned standard value


# User defined

Version 0505

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